Restriction map for
pFN21AE1727
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccII AciI AclI
AcyI AfaI AflII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BseRI
BsiEI BsiWI BsiYI BslI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstF5I
BstPI BstUI BstXI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FokI FseI FspI HaeII HaeIII
HgaI HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MscI MslI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI PvuII RsaI RsrII
SacI SacII SalI Sau96I SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SphI Sse8387I SspBI StuI SwaI
Tsp45I Tth111I Van91I XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccI 282 GTATAC GT^[AC][GT]AC
AccIII 10 TCCGGA T^CCGGA
AcsI 607 GAATTT [GA]^AATT[CT]
AflIII 324 ACATGT A^C[GA][CT]GT
Alw44I 596 GTGCAC G^TGCAC
AlwI 109 GGATC GGATC(4/5)
AlwNI 382 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 596 GTGCAC G^TGCAC
ApoI 607 GAATTT [GA]^AATT[CT]
AspHI 596 GTGCAC G[AT]GC[AT]^C
BbvCI 427 GCTGAGG CCTCAGC(-5/-2)
BbvI 660 GCAGC GCAGC(8/12)
BglII 99 AGATCT A^GATCT
BmyI 596 GTGCAC G[AGT]GC[ACT]^C
BsaJI 287 CCAAGG C^C[ACGT][ACGT]GG
BsaWI 10 TCCGGA [AT]^CCGG[AT]
BseAI 10 TCCGGA T^CCGGA
BsgI 208 GTGCAG GTGCAG(16/14)
BsiHKAI 596 GTGCAC G[AT]GC[AT]^C
Bsp1286I 596 GTGCAC G[AGT]GC[ACT]^C
BspEI 10 TCCGGA T^CCGGA
BspHI 576 TCATGA T^CATGA
BspLU11I 324 ACATGT A^CATGT
BsrI 592 ACTGG ACTGG(1/-1)
BssKI 26 CCAGG ^CC[ACGT]GG
Bst1107I 282 GTATAC GTA^TAC
BstAPI 210 GCAGAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 26 CCAGG CC^[AT]GG
BstYI 99 AGATCT [GA]^GATC[CT]
BstZ17I 282 GTATAC GTA^TAC
DraI 610 TTTAAA TTT^AAA
EcoRII 26 CCAGG ^CC[AT]GG
EcoT14I 287 CCAAGG C^C[AT][AT]GG
Fnu4HI 660 GCAGC GC^[ACGT]GC
HapII 11 CCGG C^CGG
HincII 235 GTTGAC GT[CT]^[GA]AC
HindII 235 GTTGAC GT[CT]^[GA]AC
HindIII 358 AAGCTT A^AGCTT
HpaII 11 CCGG C^CGG
HphI 202 GGTGA GGTGA(8/7)
ItaI 660 GCAGC GC^[ACGT]GC
MaeII 156 ACGT A^CGT
MaeIII 308 GTAAC ^GT[ACGT]AC
MflI 99 AGATCT [GA]^GATC[CT]
MroI 10 TCCGGA T^CCGGA
MspI 11 CCGG C^CGG
MvaI 26 CCAGG CC^[AT]GG
NspI 324 ACATGT [GA]CATG^[CT]
PshAI 138 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 576 TCATGA T^CATGA
SapI 250 GCTCTTC GCTCTTC(1/4)
ScrFI 26 CCAGG CC^[ACGT]GG
SpeI 304 ACTAGT A^CTAGT
SspI 371 AATATT AAT^ATT
StyI 287 CCAAGG C^C[AT][AT]GG
TaqI 462 TCGA T^CGA
TseI 660 GCAGC G^C[AT]GC
TspRI 403 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 462 TCGA T^CGA
XbaI 490 TCTAGA T^CTAGA
XhoII 99 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 8 enzymes) #
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AluI 359 AGCT AG^CT
426 AGCT
BbsI 135 GAAGAC GAAGAC(2/6)
171 GTCTTC
BpuAI 135 GAAGAC GAAGAC(2/6)
171 GTCTTC
HinfI 477 GATTC G^A[ACGT]TC
573 GAATC
MwoI 210 GCAGAATATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
250 GCTCTTCTTGC
PstI 140 CTGCAG CTGCA^G
176 CTGCAG
SfcI 140 CTGCAG C^T[GA][CT]AG
176 CTGCAG
TfiI 477 GATTC G^A[AT]TC
573 GAATC
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# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
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BfaI BsmAI DdeI DpnII EarI
Ksp632I MaeI MboI MboII MnlI
MseI NdeII NlaIII Sau3AI Tru9I
Tsp509I