Restriction map for
pFN21AB7520
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfaI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MaeIII MamI
MfeI MluI MslI MunI MvnI
MwoI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI TaqI Tsp45I Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AciI 220 GCGG CCGC(-3/-1)
AcsI 147 AAATTC [GA]^AATT[CT]
AflIII 4 ACGTGT A^C[GA][CT]GT
ApoI 147 AAATTC [GA]^AATT[CT]
Asp700I 343 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 506 TGGCCA TGG^CCA
BbsI 483 GAAGAC GAAGAC(2/6)
BclI 657 TGATCA T^GATCA
BmyI 512 GGGCAC G[AGT]GC[ACT]^C
BpuAI 483 GAAGAC GAAGAC(2/6)
BseRI 178 GAGGAG GAGGAG(10/8)
BsmI 687 GAATGC GAATGC(1/-1)
Bsp1286I 512 GGGCAC G[AGT]GC[ACT]^C
BstF5I 608 CATCC GGATG(2/0)
DraI 675 TTTAAA TTT^AAA
DraIII 641 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 506 TGGCCA [CT]^GGCC[GA]
EarI 293 CTCTTC CTCTTC(1/4)
FbaI 657 TGATCA T^GATCA
FokI 608 CATCC GGATG(9/13)
HphI 732 TCACC GGTGA(8/7)
Ksp632I 293 CTCTTC CTCTTC(1/4)
MaeII 4 ACGT A^CGT
MluNI 506 TGGCCA TGG^CCA
MscI 506 TGGCCA TGG^CCA
NdeI 707 CATATG CA^TATG
NlaIV 180 GGAGCC GG[ACGT]^[ACGT]CC
PflMI 498 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 599 GACTC GAGTC(4/5)
PstI 256 CTGCAG CTGCA^G
ScaI 86 AGTACT AGT^ACT
SspI 241 AATATT AAT^ATT
TfiI 355 GAATC G^A[AT]TC
Van91I 498 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 343 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccIII 369 TCCGGA T^CCGGA
554 TCCGGA
AfaI 87 GTAC GT^AC
283 GTAC
BbvI 155 GCTGC GCAGC(8/12)
217 GCTGC
BglII 78 AGATCT A^GATCT
629 AGATCT
BpmI 298 CTCCAG CTGGAG(16/14)
74 CTGGAG
BsaJI 509 CCAGGG C^C[ACGT][ACGT]GG
618 CCTGGG
BsaWI 369 TCCGGA [AT]^CCGG[AT]
554 TCCGGA
BseAI 369 TCCGGA T^CCGGA
554 TCCGGA
BspEI 369 TCCGGA T^CCGGA
554 TCCGGA
BstYI 78 AGATCT [GA]^GATC[CT]
629 AGATCT
Fnu4HI 155 GCTGC GC^[ACGT]GC
217 GCTGC
HaeIII 507 GGCC GG^CC
585 GGCC
HapII 370 CCGG C^CGG
555 CCGG
HinfI 355 GAATC G^A[ACGT]TC
599 GACTC
HpaII 370 CCGG C^CGG
555 CCGG
ItaI 155 GCTGC GC^[ACGT]GC
217 GCTGC
MflI 78 AGATCT [GA]^GATC[CT]
629 AGATCT
MroI 369 TCCGGA T^CCGGA
554 TCCGGA
MspA1I 153 CAGCTG C[AC]G^C[GT]G
277 CAGCTG
MspI 370 CCGG C^CGG
555 CCGG
PvuII 153 CAGCTG CAG^CTG
277 CAGCTG
RsaI 87 GTAC GT^AC
283 GTAC
SfcI 173 CTACAG C^T[GA][CT]AG
256 CTGCAG
TseI 155 GCTGC G^C[AT]GC
217 GCTGC
XhoII 78 AGATCT [GA]^GATC[CT]
629 AGATCT
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# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AluI BsiYI BslI BsrI BssKI
BstNI DdeI DpnII EcoRII MboI
MboII MnlI MseI MvaI NdeII
NlaIII Sau3AI ScrFI SfaNI Tru9I
Tsp509I TspRI