Restriction map for
pFN21AE6493
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp700I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaWI BseAI BseRI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI
BstEII BstPI BstXI Bsu36I CelII
Cfr10I ClaI CpoI DrdI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoT22I FbaI FseI FspI HindIII
HpaI KasI KspI MluI MluNI
MroI MscI MspA1I NaeI NarI
NcoI NdeI NgoMIV NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 911 GGTACC G^GTACC
AcyI 1206 GGCGTC G[GA]^CG[CT]C
AlwNI 1085 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1069 ATTAAT AT^TAAT
AsnI 1069 ATTAAT AT^TAAT
Asp718I 911 GGTACC G^GTACC
BbsI 304 GTCTTC GAAGAC(2/6)
BciVI 1607 GGATAC GTATCC(6/5)
BcnI 312 CCCGG CC^[GC]GG
BglII 1098 AGATCT A^GATCT
BpmI 1062 CTCCAG CTGGAG(16/14)
BpuAI 304 GTCTTC GAAGAC(2/6)
BsaAI 43 TACGTG [CT]AC^GT[GA]
BsaBI 596 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1206 GGCGTC G[GA]^CG[CT]C
BsaI 1136 GGTCTC GGTCTC(1/5)
BsmBI 382 CGTCTC CGTCTC(1/5)
Bst1107I 423 GTATAC GTA^TAC
BstYI 1098 AGATCT [GA]^GATC[CT]
BstZ17I 423 GTATAC GTA^TAC
DraI 651 TTTAAA TTT^AAA
DraII 745 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 533 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 400 CTCTTC CTCTTC(1/4)
EcoO109I 745 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 1426 GATATC GAT^ATC
FauI 55 GCGGG CCCGC(4/6)
HaeII 171 GGCGCT [GA]GCGC^[CT]
HapII 313 CCGG C^CGG
HgaI 1207 GCGTC GACGC(5/10)
Hin1I 1206 GGCGTC G[GA]^CG[CT]C
HincII 775 GTCAAC GT[CT]^[GA]AC
HindII 775 GTCAAC GT[CT]^[GA]AC
HpaII 313 CCGG C^CGG
KpnI 911 GGTACC GGTAC^C
Ksp632I 400 CTCTTC CTCTTC(1/4)
MaeII 44 ACGT A^CGT
MamI 596 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1316 CAATTG C^AATTG
MflI 1098 AGATCT [GA]^GATC[CT]
MspI 313 CCGG C^CGG
MunI 1316 CAATTG C^AATTG
NciI 312 CCCGG CC^[GC]GG
NheI 187 GCTAGC G^CTAGC
NotI 143 GCGGCCGC GC^GGCCGC
PpuMI 745 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 1069 ATTAAT AT^TAAT
SacI 1376 GAGCTC GAGCT^C
SpeI 1495 ACTAGT A^CTAGT
SphI 59 GCATGC GCATG^C
TaqI 363 TCGA T^CGA
TthHB8I 363 TCGA T^CGA
XhoII 1098 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AccI 232 GTCTAC GT^[AC][GT]AC
423 GTATAC
AlwI 925 GATCC GGATC(4/5)
581 GGATC
AspHI 1322 GAGCAC G[AT]GC[AT]^C
1376 GAGCTC
BfaI 188 CTAG C^TAG
1496 CTAG
BsiEI 74 CGGCCG CG[GA][CT]^CG
144 CGGCCG
BsiHKAI 1322 GAGCAC G[AT]GC[AT]^C
1376 GAGCTC
BstF5I 602 CATCC GGATG(2/0)
1738 CATCC
DsaI 10 CCACGG C^C[GA][CT]GG
167 CCGTGG
EagI 74 CGGCCG C^GGCCG
144 CGGCCG
EclXI 74 CGGCCG C^GGCCG
144 CGGCCG
Eco52I 74 CGGCCG C^GGCCG
144 CGGCCG
FokI 602 CATCC GGATG(9/13)
1738 CATCC
MaeI 188 CTAG C^TAG
1496 CTAG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AvaII BanI BanII BbvI
BmyI BsaJI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EcoRII
EcoT14I Fnu4HI HaeIII HhaI HinP1I
HinfI HphI ItaI MaeIII MboI
MboII MnlI MseI MslI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI