Restriction map for
pFN21AE6516
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# NO CUT ENZYMES ( 178 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BciVI BfaI BfrI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BseAI
BseRI BsgI BsiEI BsiWI BsmAI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MaeII MamI MboII MfeI MluI
MluNI MroI MscI MslI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AfaI 153 GTAC GT^AC
AlwI 217 GATCC GGATC(4/5)
AlwNI 121 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 71 CCTCAGG CC^T[ACGT]AGG
AvaII 176 GGACC G^G[AT]CC
BanI 496 GGCACC G^G[CT][GA]CC
BanII 550 GAGCTC G[GA]GC[CT]^C
BcgI 512 GCAGTGGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 314 TGATCA T^GATCA
BcnI 528 CCCGG CC^[GC]GG
BglI 523 GCCAGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 283 TCCGGT [AT]^CCGG[AT]
BsiYI 528 CCCGGCCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 528 CCCGGCCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 175 GGGAC GGGAC(10/14)
BsmI 7 GCATTC GAATGC(1/-1)
BstF5I 359 GGATG GGATG(2/0)
BstYI 216 AGATCC [GA]^GATC[CT]
Bsu36I 71 CCTCAGG CC^T[ACGT]AGG
Eco81I 71 CCTCAGG CC^T[ACGT]AGG
EcoT14I 402 CCTTGG C^C[AT][AT]GG
FbaI 314 TGATCA T^GATCA
FokI 359 GGATG GGATG(9/13)
HinfI 280 GACTC G^A[ACGT]TC
MflI 216 AGATCC [GA]^GATC[CT]
MseI 260 TTAA T^TAA
NciI 528 CCCGG CC^[GC]GG
NlaIII 157 CATG CATG^
PleI 280 GACTC GAGTC(4/5)
PstI 148 CTGCAG CTGCA^G
RsaI 153 GTAC GT^AC
SacI 550 GAGCTC GAGCT^C
SfaNI 360 GATGC GCATC(5/9)
SfcI 148 CTGCAG C^T[GA][CT]AG
StyI 402 CCTTGG C^C[AT][AT]GG
TaqI 54 TCGA T^CGA
Tru9I 260 TTAA T^TAA
TthHB8I 54 TCGA T^CGA
XhoII 216 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 274 AAATTT [GA]^AATT[CT]
456 AAATTC
AluI 379 AGCT AG^CT
551 AGCT
ApoI 274 AAATTT [GA]^AATT[CT]
456 AAATTC
AspHI 395 GTGCTC G[AT]GC[AT]^C
550 GAGCTC
BsiHKAI 395 GTGCTC G[AT]GC[AT]^C
550 GAGCTC
BspMI 14 ACCTGC ACCTGC(4/8)
480 ACCTGC
BsrI 436 ACTGG ACTGG(1/-1)
43 CCAGT
BstNI 220 CCTGG CC^[AT]GG
534 CCAGG
Cac8I 38 GCCAGC GC[ACGT]^[ACGT]GC
523 GCCAGC
DpnII 217 GATC ^GATC
315 GATC
DraIII 307 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
364 CACAGGGTG
EcoRII 220 CCTGG ^CC[AT]GG
534 CCAGG
HapII 284 CCGG C^CGG
529 CCGG
HpaII 284 CCGG C^CGG
529 CCGG
HphI 27 GGTGA GGTGA(8/7)
399 TCACC
MaeIII 28 GTGAC ^GT[ACGT]AC
160 GTCAC
MboI 217 GATC ^GATC
315 GATC
MspI 284 CCGG C^CGG
529 CCGG
MvaI 220 CCTGG CC^[AT]GG
534 CCAGG
NdeII 217 GATC ^GATC
315 GATC
NlaIV 175 GGGACC GG[ACGT]^[ACGT]CC
496 GGCACC
Sau3AI 217 GATC ^GATC
315 GATC
Tsp45I 28 GTGAC ^GT[GC]AC
160 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AciI BbvI BmyI BsaJI Bsp1286I
BssKI Cfr13I DdeI Fnu4HI HaeIII
ItaI MnlI MwoI Sau96I ScrFI
TseI Tsp509I TspRI