Restriction map for
pFN21AE2738
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# NO CUT ENZYMES ( 81 enzymes) #
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AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvrII BciVI BfrI
BglII BlnI BsaBI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrGI
Bst1107I BstBI BstXI BstZ17I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III EcoRV EcoT22I FseI HincII
HindII HpaI KpnI MamI MfeI
MluI MslI MunI NdeI NruI
NsiI NspI NspV PacI PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SfuI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XbaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccIII 2810 TCCGGA T^CCGGA
AocI 1165 CCTAAGG CC^T[ACGT]AGG
Asp700I 1452 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 150 GGATCC G^GATCC
BbrPI 2862 CACGTG CAC^GTG
BcgI 1875 CGAGGCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1175 TGATCA T^GATCA
BsaAI 2862 CACGTG [CT]AC^GT[GA]
BseAI 2810 TCCGGA T^CCGGA
BspEI 2810 TCCGGA T^CCGGA
BspMI 2384 ACCTGC ACCTGC(4/8)
BsrDI 2739 GCAATG GCAATG(2/0)
BssHII 17 GCGCGC G^CGCGC
Bsu36I 1165 CCTAAGG CC^T[ACGT]AGG
Eco81I 1165 CCTAAGG CC^T[ACGT]AGG
EcoNI 802 CCTCGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2826 GAATTC G^AATTC
FbaI 1175 TGATCA T^GATCA
HindIII 1983 AAGCTT A^AGCTT
KspI 110 CCGCGG CCGC^GG
MroI 2810 TCCGGA T^CCGGA
NheI 3538 GCTAGC G^CTAGC
NotI 106 GCGGCCGC GC^GGCCGC
PaeR7I 70 CTCGAG C^TCGAG
PmaCI 2862 CACGTG CAC^GTG
PmlI 2862 CACGTG CAC^GTG
SacII 110 CCGCGG CCGC^GG
SexAI 1292 ACCAGGT A^CC[AT]GGT
SfiI 291 GGCCAGTCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 123 CGCCGGTG C[GA]^CCGG[CT]G
SspI 2602 AATATT AAT^ATT
XhoI 70 CTCGAG C^TCGAG
XmnI 1452 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 2403 GAATTT [GA]^AATT[CT]
2826 GAATTC
ApaI 1087 GGGCCC GGGCC^C
2849 GGGCCC
ApoI 2403 GAATTT [GA]^AATT[CT]
2826 GAATTC
AviII 1706 TGCGCA TGC^GCA
2768 TGCGCA
BbvCI 2246 CCTCAGC CCTCAGC(-5/-2)
2418 CCTCAGC
BlpI 432 GCTCAGC GC^T[ACGT]AGC
3138 GCTGAGC
Bpu1102I 432 GCTCAGC GC^T[ACGT]AGC
3138 GCTGAGC
BsaI 2444 GAGACC GGTCTC(1/5)
699 GGTCTC
BsmBI 202 CGTCTC CGTCTC(1/5)
1474 CGTCTC
BstAPI 1387 GCACCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3321 GCAACTGCTGC
BstEII 1698 GGTGACC G^GT[ACGT]ACC
1855 GGTCACC
BstPI 1698 GGTGACC G^GT[ACGT]ACC
1855 GGTCACC
BstYI 150 GGATCC [GA]^GATC[CT]
2723 AGATCC
CelII 432 GCTCAGC GC^T[ACGT]AGC
3138 GCTGAGC
EcoO65I 1698 GGTGACC G^GT[ACGT]ACC
1855 GGTCACC
FspI 1706 TGCGCA TGC^GCA
2768 TGCGCA
HgaI 3361 GACGC GACGC(5/10)
3556 GACGC
MflI 150 GGATCC [GA]^GATC[CT]
2723 AGATCC
NcoI 1479 CCATGG C^CATGG
2483 CCATGG
StuI 1518 AGGCCT AGG^CCT
2301 AGGCCT
XcmI 1130 CCAGGGAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2870 CCAGCTTGAACCTGG
XhoII 150 GGATCC [GA]^GATC[CT]
2723 AGATCC
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# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XmaCI XmaI