Restriction map for
pFN21AE5019
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII AccII AclI AcsI AflIII
AgeI Alw44I AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AviII AvrII BalI BamHI BbvCI
BcgI BclI BglI BglII BlnI
BsaBI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI Ksp632I KspI MamI
MfeI MluI MluNI MscI MslI
MunI MvnI NaeI NcoI NdeI
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfuI
SgrAI SmaI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI TfiI
TthHB8I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 809 GGTACC G^GTACC
AccI 760 GTAGAC GT^[AC][GT]AC
AccIII 227 TCCGGA T^CCGGA
AcyI 449 GGCGCC G[GA]^CG[CT]C
AflII 661 CTTAAG C^TTAAG
AhdI 171 GACCAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 210 AGCGCT AGC^GCT
Asp718I 809 GGTACC G^GTACC
AspEI 171 GACCAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 63 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 802 CCCGAG C^[CT]CG[GA]G
BbeI 449 GGCGCC GGCGC^C
BbrPI 385 CACGTG CAC^GTG
BbsI 892 GTCTTC GAAGAC(2/6)
BciVI 791 GGATAC GTATCC(6/5)
BcnI 693 CCGGG CC^[GC]GG
BfrI 661 CTTAAG C^TTAAG
BlpI 475 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 475 GCTGAGC GC^T[ACGT]AGC
BpuAI 892 GTCTTC GAAGAC(2/6)
BsaHI 449 GGCGCC G[GA]^CG[CT]C
BsaI 749 GAGACC GGTCTC(1/5)
BsaWI 227 TCCGGA [AT]^CCGG[AT]
BseAI 227 TCCGGA T^CCGGA
BsgI 382 CTGCAC GTGCAG(16/14)
BsoBI 802 CCCGAG C^[CT]CG[GA]G
BspEI 227 TCCGGA T^CCGGA
BstAPI 407 GCACTCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 846 CCACCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 475 GCTGAGC GC^T[ACGT]AGC
DdeI 476 CTGAG C^T[ACGT]AG
Eam1105I 171 GACCAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 210 AGCGCT AGC^GCT
EcoNI 682 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 449 GGCGCC G[GA]^CG[CT]C
HinfI 298 GACTC G^A[ACGT]TC
KasI 449 GGCGCC G^GCGCC
KpnI 809 GGTACC GGTAC^C
MroI 227 TCCGGA T^CCGGA
MspA1I 814 CAGCTG C[AC]G^C[GT]G
NarI 449 GGCGCC GG^CGCC
NciI 693 CCGGG CC^[GC]GG
NheI 135 GCTAGC G^CTAGC
NlaIII 367 CATG CATG^
PflFI 63 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 298 GACTC GAGTC(4/5)
PmaCI 385 CACGTG CAC^GTG
PmlI 385 CACGTG CAC^GTG
PvuII 814 CAGCTG CAG^CTG
SacI 119 GAGCTC GAGCT^C
SfoI 449 GGCGCC GGC^GCC
SnaBI 757 TACGTA TAC^GTA
StuI 1034 AGGCCT AGG^CCT
Tsp45I 292 GTCAC ^GT[GC]AC
Tsp509I 820 AATT ^AATT
Tth111I 63 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BsaAI 385 CACGTG [CT]AC^GT[GA]
757 TACGTA
BsmAI 231 GAGAC GTCTC(1/5)
749 GAGAC
BsrDI 867 CATTGC GCAATG(2/0)
995 GCAATG
BstYI 422 GGATCT [GA]^GATC[CT]
665 AGATCC
CfoI 211 GCGC GCG^C
450 GCGC
HaeII 210 AGCGCT [GA]GCGC^[CT]
449 GGCGCC
HapII 228 CCGG C^CGG
693 CCGG
HhaI 211 GCGC GCG^C
450 GCGC
HinP1I 211 GCGC G^CGC
450 GCGC
HpaII 228 CCGG C^CGG
693 CCGG
MaeII 386 ACGT A^CGT
758 ACGT
MaeIII 292 GTCAC ^GT[ACGT]AC
601 GTTAC
MboII 425 TCTTC GAAGA(8/7)
893 TCTTC
MflI 422 GGATCT [GA]^GATC[CT]
665 AGATCC
MseI 511 TTAA T^TAA
662 TTAA
MspI 228 CCGG C^CGG
693 CCGG
PflMI 256 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
768 CCAGGAACTGG
PpuMI 457 GGGTCCC [GA]G^G[AT]CC[CT]
706 AGGACCC
Tru9I 511 TTAA T^TAA
662 TTAA
Van91I 256 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
768 CCAGGAACTGG
XhoII 422 GGATCT [GA]^GATC[CT]
665 AGATCC
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BanI BanII BbvI
BfaI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DpnII DraII EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HphI ItaI MaeI MboI MnlI
MvaI MwoI NdeII NlaIV PstI
RsaI Sau3AI Sau96I ScrFI SfcI
SmlI StyI TseI TspRI