Restriction map for
pFN21AE0233
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccIII AcsI AflII
AgeI AhdI Alw44I AocI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BcgI BclI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII Eam1105I
EarI Eco81I EcoO65I EcoRI EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI Ksp632I MamI
MfeI MluI MluNI MroI MscI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 25 enzymes) #
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AccI 577 GTCTAC GT^[AC][GT]AC
AclI 881 AACGTT AA^CGTT
AflIII 1040 ACATGT A^C[GA][CT]GT
BciVI 659 GTATCC GTATCC(6/5)
BsaI 418 GAGACC GGTCTC(1/5)
BsiEI 71 CGGCCG CG[GA][CT]^CG
BsmBI 442 GAGACG CGTCTC(1/5)
BspLU11I 1040 ACATGT A^CATGT
BspMI 581 ACCTGC ACCTGC(4/8)
BsrBI 663 CCGCTC CCGCTC(-3/-3)
DrdI 802 GACCTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 71 CGGCCG C^GGCCG
EclXI 71 CGGCCG C^GGCCG
Eco52I 71 CGGCCG C^GGCCG
EcoNI 387 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 944 GATATC GAT^ATC
KspI 83 CCGCGG CCGC^GG
MaeII 882 ACGT A^CGT
MboII 672 GAAGA GAAGA(8/7)
Psp1406I 881 AACGTT AA^CGTT
SacII 83 CCGCGG CCGC^GG
SmaI 59 CCCGGG CCC^GGG
XcmI 751 CCACAGAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 59 CCCGGG C^CCGGG
XmaI 59 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwNI 304 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
334 CAGATCCTG
Aor51HI 137 AGCGCT AGC^GCT
1001 AGCGCT
ApaI 78 GGGCCC GGGCC^C
1105 GGGCCC
AspHI 4 GTGCTC G[AT]GC[AT]^C
916 GAGCAC
BanII 78 GGGCCC G[GA]GC[CT]^C
1105 GGGCCC
BfaI 226 CTAG C^TAG
868 CTAG
BglII 245 AGATCT A^GATCT
587 AGATCT
BseRI 37 CTCCTC GAGGAG(10/8)
391 GAGGAG
BsgI 682 CTGCAC GTGCAG(16/14)
1075 GTGCAG
BsiHKAI 4 GTGCTC G[AT]GC[AT]^C
916 GAGCAC
BsmAI 418 GAGAC GTCTC(1/5)
442 GAGAC
BsrFI 146 GCCGGC [GA]^CCGG[CT]
644 GCCGGC
BstAPI 303 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
585 GCAGATCTTGC
BstF5I 654 CATCC GGATG(2/0)
844 CATCC
Cfr10I 146 GCCGGC [GA]^CCGG[CT]
644 GCCGGC
DdeI 693 CTCAG C^T[ACGT]AG
998 CTAAG
DsaI 83 CCGCGG C^C[GA][CT]GG
161 CCGTGG
Eco47III 137 AGCGCT AGC^GCT
1001 AGCGCT
EcoT14I 314 CCAAGG C^C[AT][AT]GG
503 CCAAGG
FokI 654 CATCC GGATG(9/13)
844 CATCC
HgaI 364 GACGC GACGC(5/10)
648 GCGTC
HinfI 863 GAGTC G^A[ACGT]TC
928 GAGTC
MaeI 226 CTAG C^TAG
868 CTAG
MaeIII 650 GTCAC ^GT[ACGT]AC
811 GTCAC
NaeI 146 GCCGGC GCC^GGC
644 GCCGGC
NgoMIV 146 GCCGGC G^CCGGC
644 GCCGGC
NspI 1040 ACATGT [GA]CATG^[CT]
1142 ACATGC
PleI 863 GAGTC GAGTC(4/5)
928 GAGTC
StyI 314 CCAAGG C^C[AT][AT]GG
503 CCAAGG
Tsp45I 650 GTCAC ^GT[GC]AC
811 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaI AvaII BanI BbeI
BbvI BcnI BmyI BpmI BsaHI
BsaJI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrI BssKI BstNI BstUI
BstYI Cac8I CfoI Cfr13I DpnII
DraII EaeI EcoO109I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII HphI ItaI
KasI MboI MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
TaqI TseI TspRI TthHB8I XhoII