Restriction map for
pFN21AE3147
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AccI AccII AclI AflII AflIII
AgeI AlwI AlwNI AocI Aor51HI
AscI Asp700I AspI AvaI AviII
BamHI BbeI BbvCI BciVI BfrI
BsaI BsiWI BsmBI BsmI BsoBI
BspDI BspLU11I BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DrdI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoT22I FseI FspI HincII HindII
HpaI KasI Ksp632I KspI MfeI
MluI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TspRI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 78 enzymes) #
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AatII 400 GACGTC GACGT^C
Acc65I 670 GGTACC G^GTACC
AccIII 557 TCCGGA T^CCGGA
AcsI 936 GAATTC [GA]^AATT[CT]
AhdI 62 GACATTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 651 GTGCAC G^TGCAC
ApaI 113 GGGCCC GGGCC^C
ApaLI 651 GTGCAC G^TGCAC
ApoI 936 GAATTC [GA]^AATT[CT]
AseI 199 ATTAAT AT^TAAT
AsnI 199 ATTAAT AT^TAAT
Asp718I 670 GGTACC G^GTACC
AspEI 62 GACATTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 651 GTGCAC G[AT]GC[AT]^C
AvrII 420 CCTAGG C^CTAGG
BalI 885 TGGCCA TGG^CCA
BanI 670 GGTACC G^G[CT][GA]CC
BbrPI 757 CACGTG CAC^GTG
BcgI 84 GCAACTTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 497 TGATCA T^GATCA
BglI 298 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 645 AGATCT A^GATCT
BlnI 420 CCTAGG C^CTAGG
BlpI 1015 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1015 GCTGAGC GC^T[ACGT]AGC
BsaAI 757 CACGTG [CT]AC^GT[GA]
BsaBI 541 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 557 TCCGGA T^CCGGA
BseRI 803 CTCCTC GAGGAG(10/8)
BsgI 1033 CTGCAC GTGCAG(16/14)
BsiEI 28 CGGTCG CG[GA][CT]^CG
BsiHKAI 651 GTGCAC G[AT]GC[AT]^C
Bsp1407I 254 TGTACA T^GTACA
BspEI 557 TCCGGA T^CCGGA
BspHI 266 TCATGA T^CATGA
BspMI 797 ACCTGC ACCTGC(4/8)
BsrGI 254 TGTACA T^GTACA
BsrI 855 ACTGG ACTGG(1/-1)
BstEII 180 GGTAACC G^GT[ACGT]ACC
BstPI 180 GGTAACC G^GT[ACGT]ACC
BstXI 449 CCAACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 645 AGATCT [GA]^GATC[CT]
Cac8I 887 GCCAGC GC[ACGT]^[ACGT]GC
CelII 1015 GCTGAGC GC^T[ACGT]AGC
DsaI 927 CCGTGG C^C[GA][CT]GG
EaeI 885 TGGCCA [CT]^GGCC[GA]
Eam1105I 62 GACATTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 180 GGTAACC G^GT[ACGT]ACC
EcoRI 936 GAATTC G^AATTC
FauI 239 CCCGC CCCGC(4/6)
FbaI 497 TGATCA T^GATCA
HaeII 3 GGCGCT [GA]GCGC^[CT]
HgaI 636 GCGTC GACGC(5/10)
HindIII 130 AAGCTT A^AGCTT
KpnI 670 GGTACC GGTAC^C
MamI 541 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 645 AGATCT [GA]^GATC[CT]
MluNI 885 TGGCCA TGG^CCA
MroI 557 TCCGGA T^CCGGA
MscI 885 TGGCCA TGG^CCA
MseI 200 TTAA T^TAA
MslI 946 CATATTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 889 CAGCTG C[AC]G^C[GT]G
PflMI 723 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 757 CACGTG CAC^GTG
PmlI 757 CACGTG CAC^GTG
PshBI 199 ATTAAT AT^TAAT
PvuII 889 CAGCTG CAG^CTG
RcaI 266 TCATGA T^CATGA
SexAI 455 ACCTGGT A^CC[AT]GGT
SmlI 768 CTTGAG C^T[CT][GA]AG
SspBI 254 TGTACA T^GTACA
TaqI 966 TCGA T^CGA
TfiI 846 GATTC G^A[AT]TC
Tru9I 200 TTAA T^TAA
TthHB8I 966 TCGA T^CGA
Van91I 723 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 645 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AciI 240 CCGC CCGC(-3/-1)
393 CCGC
AcyI 400 GACGTC G[GA]^CG[CT]C
635 GGCGTC
AvaII 512 GGACC G^G[AT]CC
1044 GGTCC
BfaI 308 CTAG C^TAG
421 CTAG
BpmI 9 CTCCAG CTGGAG(16/14)
745 CTCCAG
BsaHI 400 GACGTC G[GA]^CG[CT]C
635 GGCGTC
BsaWI 557 TCCGGA [AT]^CCGG[AT]
629 TCCGGT
BsmFI 1069 GGGAC GGGAC(10/14)
567 GTCCC
BstF5I 603 CATCC GGATG(2/0)
724 CATCC
CfoI 4 GCGC GCG^C
1077 GCGC
DpnII 498 GATC ^GATC
646 GATC
DraII 112 GGGGCCC [GA]G^G[ACGT]CC[CT]
113 GGGCCCC
DraIII 405 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1036 CACAACGTG
EcoO109I 112 GGGGCCC [GA]G^G[ACGT]CC[CT]
113 GGGCCCC
EcoRV 541 GATATC GAT^ATC
691 GATATC
EcoT14I 420 CCTAGG C^C[AT][AT]GG
1065 CCTTGG
FokI 603 CATCC GGATG(9/13)
724 CATCC
HhaI 4 GCGC GCG^C
1077 GCGC
Hin1I 400 GACGTC G[GA]^CG[CT]C
635 GGCGTC
HinP1I 4 GCGC G^CGC
1077 GCGC
MaeI 308 CTAG C^TAG
421 CTAG
MboI 498 GATC ^GATC
646 GATC
NdeII 498 GATC ^GATC
646 GATC
PleI 626 GACTC GAGTC(4/5)
831 GACTC
PstI 988 CTGCAG CTGCA^G
999 CTGCAG
Sau3AI 498 GATC ^GATC
646 GATC
SfaNI 295 GATGC GCATC(5/9)
619 GATGC
SfcI 988 CTGCAG C^T[GA][CT]AG
999 CTGCAG
StyI 420 CCTAGG C^C[AT][AT]GG
1065 CCTTGG
Tsp509I 579 AATT ^AATT
937 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AfaI AluI BanII BbsI BbvI
BcnI BmyI BpuAI BsaJI BsiYI
BslI BsmAI Bsp1286I BssKI BstNI
Cfr13I DdeI EcoRII Fnu4HI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MaeIII MboII MnlI MspI
MvaI MwoI NciI NlaIII NlaIV
RsaI Sau96I ScrFI TseI Tsp45I