Restriction map for
pFN21AE3873
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BpuAI BsaI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspMI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DsaI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp509I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AatII 88 GACGTC GACGT^C
AcyI 88 GACGTC G[GA]^CG[CT]C
AflIII 498 ACATGT A^C[GA][CT]GT
Aor51HI 716 AGCGCT AGC^GCT
Asp700I 496 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 519 GGTGCC G^G[CT][GA]CC
BglII 409 AGATCT A^GATCT
BlpI 142 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 142 GCTCAGC GC^T[ACGT]AGC
BsaAI 16 TACGTG [CT]AC^GT[GA]
BsaBI 633 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 88 GACGTC G[GA]^CG[CT]C
BspLU11I 498 ACATGT A^CATGT
BstXI 240 CCAGGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 142 GCTCAGC GC^T[ACGT]AGC
EarI 598 CTCTTC CTCTTC(1/4)
Eco47III 716 AGCGCT AGC^GCT
Hin1I 88 GACGTC G[GA]^CG[CT]C
HincII 253 GTCAAC GT[CT]^[GA]AC
HindII 253 GTCAAC GT[CT]^[GA]AC
Ksp632I 598 CTCTTC CTCTTC(1/4)
MamI 633 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 284 TTAA T^TAA
MslI 576 CACAGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 498 ACATGT [GA]CATG^[CT]
PshAI 195 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 790 CTGCAG CTGCA^G
SacI 1224 GAGCTC GAGCT^C
SfcI 790 CTGCAG C^T[GA][CT]AG
SmlI 1183 CTTGAG C^T[CT][GA]AG
TfiI 1005 GATTC G^A[AT]TC
Tru9I 284 TTAA T^TAA
XmnI 496 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BalI 779 TGGCCA TGG^CCA
839 TGGCCA
BanII 243 GGGCTC G[GA]GC[CT]^C
1224 GAGCTC
BsaWI 987 ACCGGA [AT]^CCGG[AT]
1409 ACCGGA
BsgI 277 CTGCAC GTGCAG(16/14)
748 GTGCAG
BsmAI 91 GTCTC GTCTC(1/5)
905 GTCTC
BsmBI 90 CGTCTC CGTCTC(1/5)
904 CGTCTC
BsrBI 729 CCGCTC CCGCTC(-3/-3)
1186 GAGCGG
BsrDI 738 CATTGC GCAATG(2/0)
299 GCAATG
BsrFI 1370 ACCGGC [GA]^CCGG[CT]
1427 ACCGGC
Cfr10I 1370 ACCGGC [GA]^CCGG[CT]
1427 ACCGGC
DraII 1110 GGGTCCT [GA]G^G[ACGT]CC[CT]
1301 AGGACCT
DrdI 82 GACTCAGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1103 GACTTCTGGGTC
EaeI 779 TGGCCA [CT]^GGCC[GA]
839 TGGCCA
EcoNI 96 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1272 CCTGGAGGAGG
EcoO109I 1110 GGGTCCT [GA]G^G[ACGT]CC[CT]
1301 AGGACCT
EcoT14I 1432 CCTTGG C^C[AT][AT]GG
1439 CCAAGG
HaeII 365 AGCGCC [GA]GCGC^[CT]
716 AGCGCT
MboII 599 TCTTC GAAGA(8/7)
1249 TCTTC
MluNI 779 TGGCCA TGG^CCA
839 TGGCCA
MscI 779 TGGCCA TGG^CCA
839 TGGCCA
NdeI 509 CATATG CA^TATG
962 CATATG
PflMI 363 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1087 CCAGCCCCTGG
PpuMI 1110 GGGTCCT [GA]G^G[AT]CC[CT]
1301 AGGACCT
StyI 1432 CCTTGG C^C[AT][AT]GG
1439 CCAAGG
Van91I 363 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1087 CCAGCCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MflI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SmaI TaqI TseI Tsp45I TspRI
TthHB8I XcmI XhoII XmaCI XmaI