##################################
# NO CUT ENZYMES (  198 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglII       BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmAI       BsmBI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        HpaII       
HphI        KasI        KpnI        KspI        MaeI        
MaeII       MamI        MfeI        MluI        MroI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AfaI          140  GTAC                 GT^AC
AlwI          339  GATCC                GGATC(4/5)
AlwNI          85  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII         252  GGTCC                G^G[AT]CC
BalI           53  TGGCCA               TGG^CCA
BglI           46  GCCATCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI          387  CTGCAC               GTGCAG(16/14)
BstYI         338  AGATCC               [GA]^GATC[CT]
DraII         251  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EaeI           53  TGGCCA               [CT]^GGCC[GA]
EarI          211  CTCTTC               CTCTTC(1/4)
EcoO109I      251  AGGTCCC              [GA]G^G[ACGT]CC[CT]
HindIII       229  AAGCTT               A^AGCTT
HinfI         209  GACTC                G^A[ACGT]TC
Ksp632I       211  CTCTTC               CTCTTC(1/4)
MflI          338  AGATCC               [GA]^GATC[CT]
MluNI          53  TGGCCA               TGG^CCA
MscI           53  TGGCCA               TGG^CCA
MseI          350  TTAA                 T^TAA
MslI          151  CACACCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         85  CAGCTG               C[AC]G^C[GT]G
NlaIII        144  CATG                 CATG^
NlaIV         252  GGTCCC               GG[ACGT]^[ACGT]CC
PleI          209  GACTC                GAGTC(4/5)
PpuMI         251  AGGTCCC              [GA]G^G[AT]CC[CT]
PvuII          85  CAGCTG               CAG^CTG
RsaI          140  GTAC                 GT^AC
Tru9I         350  TTAA                 T^TAA
XcmI          332  CCAAGAAGATCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         338  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

BbvI          245  GCAGC                GCAGC(8/12)
               87  GCTGC               
BsrI          365  ACTGG                ACTGG(1/-1)
              155  CCAGT               
Cfr13I        252  GGTCC                G^G[ACGT]CC
              372  GGCCC               
DdeI          257  CTCAG                C^T[ACGT]AG
              392  CTGAG               
Fnu4HI         87  GCTGC                GC^[ACGT]GC
              245  GCAGC               
HaeIII         54  GGCC                 GG^CC
              372  GGCC                
ItaI           87  GCTGC                GC^[ACGT]GC
              245  GCAGC               
MaeIII        275  GTGAC                ^GT[ACGT]AC
              287  GTGAC               
MboII         336  GAAGA                GAAGA(8/7)
              212  TCTTC               
Sau96I        252  GGTCC                G^G[ACGT]CC
              372  GGCCC               
SfaNI          32  GCATC                GCATC(5/9)
              116  GCATC               
TseI           87  GCTGC                G^C[AT]GC
              245  GCAGC               
Tsp45I        275  GTGAC                ^GT[GC]AC
              287  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AluI        BsmFI       BssKI       BstF5I      BstNI       
DpnII       EcoRII      FokI        MboI        MnlI        
MvaI        MwoI        NdeII       Sau3AI      ScrFI       
Tsp509I     TspRI