Restriction map for
pFN21AE1922
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspI
AvaI AvrII BalI BbeI BbrPI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT14I EcoT22I
FbaI FseI HaeII Hin1I HincII
HindII HindIII HpaI KasI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MseI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspI StyI SwaI Tru9I
Tsp45I Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 885 GGTACC G^GTACC
AlwNI 1078 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 885 GGTACC G^GTACC
AspHI 47 GAGCAC G[AT]GC[AT]^C
AvaII 1012 GGACC G^G[AT]CC
AviII 951 TGCGCA TGC^GCA
BanII 1139 GAGCCC G[GA]GC[CT]^C
BbsI 1170 GAAGAC GAAGAC(2/6)
BbvCI 523 GCTGAGG CCTCAGC(-5/-2)
BcnI 1091 CCCGG CC^[GC]GG
BglII 265 AGATCT A^GATCT
BpuAI 1170 GAAGAC GAAGAC(2/6)
BsaI 194 GGTCTC GGTCTC(1/5)
BseRI 53 CTCCTC GAGGAG(10/8)
BsiHKAI 47 GAGCAC G[AT]GC[AT]^C
BsmBI 98 GAGACG CGTCTC(1/5)
Bsp1407I 1257 TGTACA T^GTACA
BspMI 882 GCAGGT ACCTGC(4/8)
BsrBI 190 GAGCGG CCGCTC(-3/-3)
BsrDI 1261 CATTGC GCAATG(2/0)
BsrGI 1257 TGTACA T^GTACA
BstAPI 1077 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 952 GCGC GCG^C
DrdI 948 GACTGCGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 240 TGGCCG [CT]^GGCC[GA]
EcoRI 32 GAATTC G^AATTC
FspI 951 TGCGCA TGC^GCA
HapII 1092 CCGG C^CGG
HhaI 952 GCGC GCG^C
HinP1I 952 GCGC G^CGC
HpaII 1092 CCGG C^CGG
KpnI 885 GGTACC GGTAC^C
MaeIII 575 GTAAC ^GT[ACGT]AC
MspI 1092 CCGG C^CGG
NciI 1091 CCCGG CC^[GC]GG
PpuMI 1011 AGGACCC [GA]G^G[AT]CC[CT]
SbfI 1047 CCTGCAGG CCTGCA^GG
SmlI 453 CTCAAG C^T[CT][GA]AG
Sse8387I 1047 CCTGCAGG CCTGCA^GG
SspBI 1257 TGTACA T^GTACA
StuI 412 AGGCCT AGG^CCT
TaqI 57 TCGA T^CGA
TthHB8I 57 TCGA T^CGA
XcmI 1144 CCAGACATTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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Asp700I 28 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
245 GAAAGGATTC
BamHI 607 GGATCC G^GATCC
1193 GGATCC
BanI 361 GGTGCC G^G[CT][GA]CC
885 GGTACC
BcgI 102 CGATCATTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
942 CGAAGAGACTGC
BfaI 556 CTAG C^TAG
1009 CTAG
BmyI 47 GAGCAC G[AGT]GC[ACT]^C
1139 GAGCCC
BpmI 299 CTCCAG CTGGAG(16/14)
1223 CTCCAG
BsaJI 1090 CCCCGG C^C[ACGT][ACGT]GG
1154 CCTGGG
Bsp1286I 47 GAGCAC G[AGT]GC[ACT]^C
1139 GAGCCC
BstNI 358 CCTGG CC^[AT]GG
1154 CCTGG
EarI 171 CTCTTC CTCTTC(1/4)
943 GAAGAG
EcoRII 358 CCTGG ^CC[AT]GG
1154 CCTGG
HgaI 982 GACGC GACGC(5/10)
354 GCGTC
Ksp632I 171 CTCTTC CTCTTC(1/4)
943 GAAGAG
MaeI 556 CTAG C^TAG
1009 CTAG
MslI 64 CATCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
903 CACTGAAATG
MvaI 358 CCTGG CC^[AT]GG
1154 CCTGG
PleI 649 GAGTC GAGTC(4/5)
859 GAGTC
XmnI 28 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
245 GAAAGGATTC
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstF5I BstYI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I FauI Fnu4HI FokI HaeIII
HinfI HphI ItaI MboI MboII
MflI MnlI MwoI NdeII NlaIII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI TseI
Tsp509I TspRI XhoII