Restriction map for
pFN21AB8524
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspEI AviII AvrII
BbrPI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsiWI BsmBI Bsp1407I BspDI BspLU11I
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstZ17I CelII Cfr10I ClaI
DraI DraIII Eam1105I Eco47III EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI MamI MfeI MluI
MunI NaeI NcoI NdeI NgoMIV
NheI NruI NsiI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI SalI SapI
SbfI SexAI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Van91I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 1314 GGTACC G^GTACC
AccI 259 GTCTAC GT^[AC][GT]AC
AccIII 38 TCCGGA T^CCGGA
AcsI 1389 GAATTT [GA]^AATT[CT]
AcyI 13 GGCGCC G[GA]^CG[CT]C
AocI 1973 CCTTAGG CC^T[ACGT]AGG
ApaI 913 GGGCCC GGGCC^C
ApoI 1389 GAATTT [GA]^AATT[CT]
Asp700I 1225 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1314 GGTACC G^GTACC
AspI 2206 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2336 TGGCCA TGG^CCA
BamHI 164 GGATCC G^GATCC
BbeI 13 GGCGCC GGCGC^C
BcgI 2352 CGATGCCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 565 CTAG C^TAG
BsaAI 723 CACGTA [CT]AC^GT[GA]
BsaHI 13 GGCGCC G[GA]^CG[CT]C
BseAI 38 TCCGGA T^CCGGA
BspEI 38 TCCGGA T^CCGGA
BspHI 1421 TCATGA T^CATGA
BssSI 1135 CACGAG CACGAG(-5/-1)
BstEII 2089 GGTGACC G^GT[ACGT]ACC
BstPI 2089 GGTGACC G^GT[ACGT]ACC
Bsu36I 1973 CCTTAGG CC^T[ACGT]AGG
CpoI 40 CGGACCG CG^G[AT]CCG
DrdI 1618 GACTCACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1973 CCTTAGG CC^T[ACGT]AGG
EcoO65I 2089 GGTGACC G^GT[ACGT]ACC
EcoT14I 1880 CCAAGG C^C[AT][AT]GG
Hin1I 13 GGCGCC G[GA]^CG[CT]C
HindIII 1195 AAGCTT A^AGCTT
HpaI 1171 GTTAAC GTT^AAC
KasI 13 GGCGCC G^GCGCC
KpnI 1314 GGTACC GGTAC^C
KspI 135 CCGCGG CCGC^GG
MaeI 565 CTAG C^TAG
MluNI 2336 TGGCCA TGG^CCA
MroI 38 TCCGGA T^CCGGA
MscI 2336 TGGCCA TGG^CCA
NarI 13 GGCGCC GG^CGCC
PaeR7I 1078 CTCGAG C^TCGAG
PflFI 2206 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2034 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1421 TCATGA T^CATGA
RsrII 40 CGGACCG CG^G[AT]CCG
SacII 135 CCGCGG CCGC^GG
ScaI 2218 AGTACT AGT^ACT
SfiI 133 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 13 GGCGCC GGC^GCC
SspI 1742 AATATT AAT^ATT
StyI 1880 CCAAGG C^C[AT][AT]GG
Tth111I 2206 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1078 CTCGAG C^TCGAG
XmnI 1225 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 136 CGCG CG^CG
2299 CGCG
AvaI 1078 CTCGAG C^[CT]CG[GA]G
2214 CCCGAG
BbvCI 900 GCTGAGG CCTCAGC(-5/-2)
2124 GCTGAGG
BsaWI 38 TCCGGA [AT]^CCGG[AT]
1769 ACCGGA
BseRI 435 GAGGAG GAGGAG(10/8)
942 GAGGAG
BsoBI 1078 CTCGAG C^[CT]CG[GA]G
2214 CCCGAG
BspMI 174 GCAGGT ACCTGC(4/8)
807 GCAGGT
BsrDI 1436 CATTGC GCAATG(2/0)
1647 CATTGC
BstAPI 525 GCACACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2347 GCAGGCGATGC
BstUI 136 CGCG CG^CG
2299 CGCG
BstYI 59 AGATCC [GA]^GATC[CT]
164 GGATCC
EarI 371 GAAGAG CTCTTC(1/4)
1164 GAAGAG
HincII 1171 GTTAAC GT[CT]^[GA]AC
2470 GTCAAC
HindII 1171 GTTAAC GT[CT]^[GA]AC
2470 GTCAAC
HphI 2089 GGTGA GGTGA(8/7)
231 TCACC
Ksp632I 371 GAAGAG CTCTTC(1/4)
1164 GAAGAG
MflI 59 AGATCC [GA]^GATC[CT]
164 GGATCC
MslI 1044 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2083 CATGTTGGTG
MvnI 136 CGCG CG^CG
2299 CGCG
NotI 131 GCGGCCGC GC^GGCCGC
970 GCGGCCGC
PpuMI 415 AGGTCCT [GA]G^G[AT]CC[CT]
859 AGGTCCT
XhoII 59 AGATCC [GA]^GATC[CT]
164 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BanI BanII BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaJI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI