Restriction map for
pFN21AE4020
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# NO CUT ENZYMES ( 187 enzymes) #
##################################
Acc65I AccI AccII AccIII AciI
AclI AfaI AflII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BlnI BlpI BmyI
Bpu1102I BsaAI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstNI BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoRV
EcoT14I EcoT22I FauI FseI FspI
HgaI HincII HindII HindIII HpaI
HphI KasI KpnI Ksp632I KspI
MfeI MluI MroI MseI MslI
MspA1I MunI MvaI MvnI MwoI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tru9I Tsp45I Tth111I
TthHB8I Van91I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 545 GACGTC GACGT^C
AcsI 199 AAATTC [GA]^AATT[CT]
AcyI 545 GACGTC G[GA]^CG[CT]C
AlwI 556 GGATC GGATC(4/5)
AlwNI 469 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 288 AGCGCT AGC^GCT
ApoI 199 AAATTC [GA]^AATT[CT]
BalI 569 TGGCCA TGG^CCA
BbsI 267 GTCTTC GAAGAC(2/6)
BbvI 460 GCAGC GCAGC(8/12)
BclI 578 TGATCA T^GATCA
BcnI 306 CCGGG CC^[GC]GG
BglII 375 AGATCT A^GATCT
BpmI 475 CTGGAG CTGGAG(16/14)
BpuAI 267 GTCTTC GAAGAC(2/6)
BsaHI 545 GACGTC G[GA]^CG[CT]C
BsmBI 543 GAGACG CGTCTC(1/5)
BsmFI 548 GTCCC GGGAC(10/14)
BssKI 306 CCGGG ^CC[ACGT]GG
BstF5I 513 CATCC GGATG(2/0)
Cac8I 420 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 289 GCGC GCG^C
DdeI 77 CTAAG C^T[ACGT]AG
EaeI 569 TGGCCA [CT]^GGCC[GA]
Eco47III 288 AGCGCT AGC^GCT
FbaI 578 TGATCA T^GATCA
Fnu4HI 460 GCAGC GC^[ACGT]GC
FokI 513 CATCC GGATG(9/13)
HaeII 288 AGCGCT [GA]GCGC^[CT]
HaeIII 570 GGCC GG^CC
HapII 306 CCGG C^CGG
HhaI 289 GCGC GCG^C
Hin1I 545 GACGTC G[GA]^CG[CT]C
HinP1I 289 GCGC G^CGC
HpaII 306 CCGG C^CGG
ItaI 460 GCAGC GC^[ACGT]GC
MaeII 546 ACGT A^CGT
MaeIII 46 GTTAC ^GT[ACGT]AC
MluNI 569 TGGCCA TGG^CCA
MscI 569 TGGCCA TGG^CCA
MspI 306 CCGG C^CGG
NciI 306 CCGGG CC^[GC]GG
NlaIII 385 CATG CATG^
NlaIV 309 GGAGCC GG[ACGT]^[ACGT]CC
PstI 458 CTGCAG CTGCA^G
ScrFI 306 CCGGG CC^[ACGT]GG
SpeI 319 ACTAGT A^CTAGT
TseI 460 GCAGC G^C[AT]GC
XbaI 159 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
BfaI 160 CTAG C^TAG
320 CTAG
BsaBI 489 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
557 GATCTAAATC
BsmAI 543 GAGAC GTCTC(1/5)
471 GTCTC
BstYI 375 AGATCT [GA]^GATC[CT]
556 GGATCT
MaeI 160 CTAG C^TAG
320 CTAG
MamI 489 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
557 GATCTAAATC
MboII 406 GAAGA GAAGA(8/7)
268 TCTTC
MflI 375 AGATCT [GA]^GATC[CT]
556 GGATCT
SfaNI 437 GATGC GCATC(5/9)
15 GCATC
SfcI 297 CTACAG C^T[GA][CT]AG
458 CTGCAG
XhoII 375 AGATCT [GA]^GATC[CT]
556 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AluI BsrI DpnII HinfI MboI
MnlI NdeII Sau3AI TfiI Tsp509I
TspRI