Restriction map for
pFN21AE3937
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# NO CUT ENZYMES ( 183 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BciVI BclI BcnI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
CpoI DdeI DraI DraII DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeII MamI MfeI MflI MluI
MroI MslI MspA1I MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 247 GTCTAC GT^[AC][GT]AC
AciI 13 GCGG CCGC(-3/-1)
AflII 292 CTTAAG C^TTAAG
AlwI 106 GATCC GGATC(4/5)
AspHI 438 GTGCTC G[AT]GC[AT]^C
AvaII 366 GGACC G^G[AT]CC
BalI 407 TGGCCA TGG^CCA
BcgI 435 CGAGTGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 292 CTTAAG C^TTAAG
BsiHKAI 438 GTGCTC G[AT]GC[AT]^C
BspDI 145 ATCGAT AT^CGAT
BspMI 378 GCAGGT ACCTGC(4/8)
BssKI 374 CCAGG ^CC[ACGT]GG
BstNI 374 CCAGG CC^[AT]GG
BstXI 410 CCACAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 366 GGACC G^G[ACGT]CC
ClaI 145 ATCGAT AT^CGAT
DsaI 164 CCATGG C^C[GA][CT]GG
EaeI 407 TGGCCA [CT]^GGCC[GA]
EcoRII 374 CCAGG ^CC[AT]GG
EcoT14I 164 CCATGG C^C[AT][AT]GG
HaeIII 408 GGCC GG^CC
HapII 507 CCGG C^CGG
HpaII 507 CCGG C^CGG
HphI 581 TCACC GGTGA(8/7)
MaeIII 331 GTGAC ^GT[ACGT]AC
MluNI 407 TGGCCA TGG^CCA
MscI 407 TGGCCA TGG^CCA
MspI 507 CCGG C^CGG
MvaI 374 CCAGG CC^[AT]GG
NcoI 164 CCATGG C^CATGG
PleI 333 GACTC GAGTC(4/5)
Sau96I 366 GGACC G^G[ACGT]CC
ScrFI 374 CCAGG CC^[ACGT]GG
SfaNI 576 GATGC GCATC(5/9)
SmlI 292 CTTAAG C^T[CT][GA]AG
SspI 30 AATATT AAT^ATT
StyI 164 CCATGG C^C[AT][AT]GG
TfiI 118 GAATC G^A[AT]TC
Tsp45I 331 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 28 enzymes) #
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BbvI 539 GCAGC GCAGC(8/12)
285 GCTGC
BfaI 175 CTAG C^TAG
304 CTAG
BmyI 214 GGGCAC G[AGT]GC[ACT]^C
438 GTGCTC
BpmI 372 CTCCAG CTGGAG(16/14)
452 CTGGAG
BsaJI 164 CCATGG C^C[ACGT][ACGT]GG
264 CCGAGG
BsgI 447 CTGCAC GTGCAG(16/14)
517 GTGCAG
BsiYI 200 CCATTCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
507 CCGGAGGATGG
BslI 200 CCATTCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
507 CCGGAGGATGG
Bsp1286I 214 GGGCAC G[AGT]GC[ACT]^C
438 GTGCTC
BsrI 24 ACTGG ACTGG(1/-1)
451 ACTGG
BstF5I 300 CATCC GGATG(2/0)
512 GGATG
DpnII 106 GATC ^GATC
312 GATC
Fnu4HI 285 GCTGC GC^[ACGT]GC
539 GCAGC
FokI 300 CATCC GGATG(9/13)
512 GGATG
HgaI 536 GACGC GACGC(5/10)
501 GCGTC
HinfI 118 GAATC G^A[ACGT]TC
333 GACTC
ItaI 285 GCTGC GC^[ACGT]GC
539 GCAGC
MaeI 175 CTAG C^TAG
304 CTAG
MboI 106 GATC ^GATC
312 GATC
MboII 193 GAAGA GAAGA(8/7)
391 GAAGA
MseI 293 TTAA T^TAA
569 TTAA
NdeII 106 GATC ^GATC
312 GATC
NspI 218 ACATGC [GA]CATG^[CT]
552 GCATGT
Sau3AI 106 GATC ^GATC
312 GATC
Tru9I 293 TTAA T^TAA
569 TTAA
TseI 285 GCTGC G^C[AT]GC
539 GCAGC
Tsp509I 416 AATT ^AATT
485 AATT
TspRI 180 CACTG CA[GC]TG[ACGT][ACGT]^
450 CACTG
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# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
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AluI Cac8I MnlI MwoI NlaIII
TaqI TthHB8I