Restriction map for
pFN21AE1811
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# NO CUT ENZYMES ( 156 enzymes) #
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Acc65I AccII AccIII AciI AclI
AflII AflIII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AvaI AviII
AvrII BanII BbeI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MroI
MslI MspA1I MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TaqI
TthHB8I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 829 GACGTC GACGT^C
AccI 976 GTAGAC GT^[AC][GT]AC
AcyI 829 GACGTC G[GA]^CG[CT]C
AfaI 897 GTAC GT^AC
AhdI 95 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 279 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 95 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 751 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 490 TGGCCA TGG^CCA
BamHI 923 GGATCC G^GATCC
BanI 889 GGCACC G^G[CT][GA]CC
BbrPI 577 CACGTG CAC^GTG
BsaAI 577 CACGTG [CT]AC^GT[GA]
BsaHI 829 GACGTC G[GA]^CG[CT]C
BsiYI 705 CCACTTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 705 CCACTTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 159 GTCCC GGGAC(10/14)
BsmI 283 GCATTC GAATGC(1/-1)
BsrDI 456 CATTGC GCAATG(2/0)
BstYI 923 GGATCC [GA]^GATC[CT]
DsaI 919 CCATGG C^C[GA][CT]GG
Eam1105I 95 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 124 CTCTTC CTCTTC(1/4)
EcoRI 67 GAATTC G^AATTC
EcoRV 960 GATATC GAT^ATC
Hin1I 829 GACGTC G[GA]^CG[CT]C
HphI 130 TCACC GGTGA(8/7)
Ksp632I 124 CTCTTC CTCTTC(1/4)
MflI 923 GGATCC [GA]^GATC[CT]
MluNI 490 TGGCCA TGG^CCA
MscI 490 TGGCCA TGG^CCA
MseI 809 TTAA T^TAA
NcoI 919 CCATGG C^CATGG
PflFI 751 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 193 GAGTC GAGTC(4/5)
PmaCI 577 CACGTG CAC^GTG
PmlI 577 CACGTG CAC^GTG
RsaI 897 GTAC GT^AC
SexAI 752 ACCAGGT A^CC[AT]GGT
SfcI 584 CTGTAG C^T[GA][CT]AG
SspI 650 AATATT AAT^ATT
Tru9I 809 TTAA T^TAA
Tth111I 751 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 923 GGATCC [GA]^GATC[CT]
XmnI 279 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 67 GAATTC [GA]^AATT[CT]
225 AAATTC
AlwI 924 GATCC GGATC(4/5)
923 GGATC
ApoI 67 GAATTC [GA]^AATT[CT]
225 AAATTC
AspHI 111 GAGCAC G[AT]GC[AT]^C
318 GAGCAC
BbsI 103 GTCTTC GAAGAC(2/6)
270 GTCTTC
BfaI 40 CTAG C^TAG
625 CTAG
BmyI 111 GAGCAC G[AGT]GC[ACT]^C
318 GAGCAC
BpuAI 103 GTCTTC GAAGAC(2/6)
270 GTCTTC
BsaI 333 GGTCTC GGTCTC(1/5)
553 GGTCTC
BsiHKAI 111 GAGCAC G[AT]GC[AT]^C
318 GAGCAC
BsmAI 334 GTCTC GTCTC(1/5)
554 GTCTC
Bsp1286I 111 GAGCAC G[AGT]GC[ACT]^C
318 GAGCAC
BsrI 11 CCAGT ACTGG(1/-1)
236 CCAGT
BstF5I 714 GGATG GGATG(2/0)
940 GGATG
DpnII 661 GATC ^GATC
924 GATC
DrdI 201 GACAGAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
439 GACCCCAAAGTC
EaeI 490 TGGCCA [CT]^GGCC[GA]
836 CGGCCA
FokI 714 GGATG GGATG(9/13)
940 GGATG
MaeI 40 CTAG C^TAG
625 CTAG
MboI 661 GATC ^GATC
924 GATC
NdeII 661 GATC ^GATC
924 GATC
PpuMI 755 AGGTCCT [GA]G^G[AT]CC[CT]
875 GGGTCCT
Sau3AI 661 GATC ^GATC
924 GATC
Tsp45I 20 GTGAC ^GT[GC]AC
832 GTCAC
XcmI 705 CCACTTCCAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
927 CCAGCAGCACGGTGG
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI AvaII BbvI BsaJI BssKI
BstNI Cac8I Cfr13I DdeI DraII
EcoO109I EcoRII EcoT14I Fnu4HI HaeIII
HinfI ItaI MaeII MaeIII MboII
MnlI MvaI MwoI NlaIII NlaIV
Sau96I ScrFI StyI TfiI TseI
Tsp509I TspRI