Restriction map for
pFN21AE6283
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AvrII BamHI BbeI
BbrPI BbvCI BciVI BclI BcnI
BfrI BglI BlnI BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HaeII HapII Hin1I
HincII HindII HindIII HpaI HpaII
KasI Ksp632I KspI MaeII MamI
MfeI MluI MroI MspI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI SwaI
TfiI Tth111I Van91I XbaI XcmI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 379 GGTACC G^GTACC
AccII 35 CGCG CG^CG
AlwNI 323 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 379 GGTACC G^GTACC
AvaI 65 CTCGAG C^[CT]CG[GA]G
BalI 488 TGGCCA TGG^CCA
BbvI 575 GCAGC GCAGC(8/12)
BcgI 57 GCATCTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 535 AGATCT A^GATCT
BlpI 5 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 5 GCTCAGC GC^T[ACGT]AGC
BsaI 248 GAGACC GGTCTC(1/5)
BsoBI 65 CTCGAG C^[CT]CG[GA]G
BsrBI 198 CCGCTC CCGCTC(-3/-3)
BstUI 35 CGCG CG^CG
BstXI 113 CCATCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 5 GCTCAGC GC^T[ACGT]AGC
DraII 518 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 86 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 488 TGGCCA [CT]^GGCC[GA]
EcoO109I 518 GGGACCC [GA]G^G[ACGT]CC[CT]
FauI 197 CCCGC CCCGC(4/6)
HgaI 17 GCGTC GACGC(5/10)
KpnI 379 GGTACC GGTAC^C
MaeIII 25 GTCAC ^GT[ACGT]AC
MluNI 488 TGGCCA TGG^CCA
MscI 488 TGGCCA TGG^CCA
MseI 173 TTAA T^TAA
MslI 114 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 274 CAGCTG C[AC]G^C[GT]G
MvnI 35 CGCG CG^CG
NspI 177 ACATGC [GA]CATG^[CT]
PaeR7I 65 CTCGAG C^TCGAG
PpuMI 518 GGGACCC [GA]G^G[AT]CC[CT]
PstI 610 CTGCAG CTGCA^G
PvuII 274 CAGCTG CAG^CTG
SbfI 609 CCTGCAGG CCTGCA^GG
SfcI 610 CTGCAG C^T[GA][CT]AG
Sse8387I 609 CCTGCAGG CCTGCA^GG
StuI 141 AGGCCT AGG^CCT
TaqI 66 TCGA T^CGA
Tru9I 173 TTAA T^TAA
TseI 575 GCAGC G^C[AT]GC
Tsp45I 25 GTCAC ^GT[GC]AC
Tsp509I 362 AATT ^AATT
TthHB8I 66 TCGA T^CGA
XhoI 65 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 380 GTAC GT^AC
583 GTAC
AlwI 193 GATCC GGATC(4/5)
593 GATCC
AspHI 126 GAGCTC G[AT]GC[AT]^C
498 GAGCTC
AviII 104 TGCGCA TGC^GCA
551 TGCGCA
BbsI 234 GAAGAC GAAGAC(2/6)
298 GAAGAC
BfaI 89 CTAG C^TAG
586 CTAG
BpmI 163 CTGGAG CTGGAG(16/14)
734 CTGGAG
BpuAI 234 GAAGAC GAAGAC(2/6)
298 GAAGAC
BsiHKAI 126 GAGCTC G[AT]GC[AT]^C
498 GAGCTC
BsmAI 248 GAGAC GTCTC(1/5)
526 GAGAC
BsmFI 518 GGGAC GGGAC(10/14)
19 GTCCC
Cac8I 94 GCTGGC GC[ACGT]^[ACGT]GC
490 GCCAGC
FspI 104 TGCGCA TGC^GCA
551 TGCGCA
MaeI 89 CTAG C^TAG
586 CTAG
RsaI 380 GTAC GT^AC
583 GTAC
SacI 126 GAGCTC GAGCT^C
498 GAGCTC
SmlI 65 CTCGAG C^T[CT][GA]AG
513 CTCAAG
TspRI 243 CAGTG CA[GC]TG[ACGT][ACGT]^
263 CACTG
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AvaII BanI BanII
BmyI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
CfoI Cfr13I DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI MboI
MboII MflI MnlI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI StyI XhoII