Restriction map for
pFN21AE3969
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaII
AvrII BamHI BanII BbeI BbrPI
BbsI BcgI BciVI BclI BfaI
BfrI BglI BlnI BpuAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MroI MunI NarI NdeI NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI StuI SwaI Tsp509I
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 61 GACGTC GACGT^C
AflIII 329 ACGCGT A^C[GA][CT]GT
Alw44I 93 GTGCAC G^TGCAC
ApaLI 93 GTGCAC G^TGCAC
BanI 563 GGCACC G^G[CT][GA]CC
BglII 648 AGATCT A^GATCT
BlpI 1054 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1054 GCTGAGC GC^T[ACGT]AGC
BsaAI 250 TACGTG [CT]AC^GT[GA]
BseRI 694 CTCCTC GAGGAG(10/8)
BssSI 1199 CACGAG CACGAG(-5/-1)
BstAPI 987 GCACGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1054 GCTGAGC GC^T[ACGT]AGC
DsaI 824 CCATGG C^C[GA][CT]GG
EcoT14I 824 CCATGG C^C[AT][AT]GG
EcoT22I 814 ATGCAT ATGCA^T
HaeII 506 AGCGCC [GA]GCGC^[CT]
MboII 47 TCTTC GAAGA(8/7)
MluI 329 ACGCGT A^CGCGT
MseI 521 TTAA T^TAA
MslI 946 CACATCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 571 GCCGGC GCC^GGC
NcoI 824 CCATGG C^CATGG
NgoMIV 571 GCCGGC G^CCGGC
NsiI 814 ATGCAT ATGCA^T
PshAI 880 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 192 CCTGCAGG CCTGCA^GG
SmaI 200 CCCGGG CCC^GGG
Sse8387I 192 CCTGCAGG CCTGCA^GG
StyI 824 CCATGG C^C[AT][AT]GG
TaqI 275 TCGA T^CGA
Tru9I 521 TTAA T^TAA
TthHB8I 275 TCGA T^CGA
XmaCI 200 CCCGGG C^CCGGG
XmaI 200 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 61 GACGTC G[GA]^CG[CT]C
109 GACGCC
AlwI 222 GATCC GGATC(4/5)
255 GATCC
AspHI 93 GTGCAC G[AT]GC[AT]^C
1057 GAGCAC
BsaHI 61 GACGTC G[GA]^CG[CT]C
109 GACGCC
BsaI 4 GAGACC GGTCTC(1/5)
878 GAGACC
BsiHKAI 93 GTGCAC G[AT]GC[AT]^C
1057 GAGCAC
BsrFI 571 GCCGGC [GA]^CCGG[CT]
1075 ACCGGC
BsrI 475 ACTGG ACTGG(1/-1)
417 CCAGT
BssHII 78 GCGCGC G^CGCGC
357 GCGCGC
BstYI 221 AGATCC [GA]^GATC[CT]
648 AGATCT
Cfr10I 571 GCCGGC [GA]^CCGG[CT]
1075 ACCGGC
DraII 428 AGGGCCT [GA]G^G[ACGT]CC[CT]
1037 AGGGCCT
EcoO109I 428 AGGGCCT [GA]G^G[ACGT]CC[CT]
1037 AGGGCCT
FauI 105 GCGGG CCCGC(4/6)
135 GCGGG
Hin1I 61 GACGTC G[GA]^CG[CT]C
109 GACGCC
MaeII 62 ACGT A^CGT
251 ACGT
MflI 221 AGATCC [GA]^GATC[CT]
648 AGATCT
PleI 1166 GACTC GAGTC(4/5)
885 GAGTC
PstI 193 CTGCAG CTGCA^G
502 CTGCAG
PvuII 531 CAGCTG CAG^CTG
1135 CAGCTG
TfiI 719 GAATC G^A[AT]TC
1103 GAATC
XcmI 903 CCAGGGGCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1109 CCAAGCGCGGCCTGG
XhoII 221 AGATCC [GA]^GATC[CT]
648 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AvaI AviII BalI BbvCI BbvI
BcnI BmyI BpmI BsaJI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EcoRII Fnu4HI FokI
FspI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TseI Tsp45I TspRI
Van91I