Restriction map for
pFN21AE1221
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI Alw44I ApaLI
ApoI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I EarI EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FokI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI Ksp632I MaeI MaeII
MamI MfeI MluI MluNI MscI
MseI MslI MunI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp509I Van91I
XbaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 682 TCCGGA T^CCGGA
AcyI 409 GACGCC G[GA]^CG[CT]C
AfaI 696 GTAC GT^AC
AluI 712 AGCT AG^CT
AlwNI 75 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 601 CCTGAGG CC^T[ACGT]AGG
Aor51HI 326 AGCGCT AGC^GCT
ApaI 268 GGGCCC GGGCC^C
AscI 169 GGCGCGCC GG^CGCGCC
AspHI 291 GTGCTC G[AT]GC[AT]^C
AspI 409 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 516 CGAATCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 595 CTGGAG CTGGAG(16/14)
BsaHI 409 GACGCC G[GA]^CG[CT]C
BsaWI 682 TCCGGA [AT]^CCGG[AT]
BseAI 682 TCCGGA T^CCGGA
BsgI 619 GTGCAG GTGCAG(16/14)
BsiHKAI 291 GTGCTC G[AT]GC[AT]^C
BspEI 682 TCCGGA T^CCGGA
BsrDI 522 CATTGC GCAATG(2/0)
BssHII 170 GCGCGC G^CGCGC
BstXI 435 CCAGGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 68 AGATCC [GA]^GATC[CT]
Bsu36I 601 CCTGAGG CC^T[ACGT]AGG
DdeI 602 CTGAG C^T[ACGT]AG
Eco47III 326 AGCGCT AGC^GCT
Eco81I 601 CCTGAGG CC^T[ACGT]AGG
Hin1I 409 GACGCC G[GA]^CG[CT]C
HinfI 517 GAATC G^A[ACGT]TC
KspI 263 CCGCGG CCGC^GG
MflI 68 AGATCC [GA]^GATC[CT]
MroI 682 TCCGGA T^CCGGA
MspA1I 263 CCGCGG C[AC]G^C[GT]G
NaeI 40 GCCGGC GCC^GGC
NgoMIV 40 GCCGGC G^CCGGC
PaeR7I 227 CTCGAG C^TCGAG
PflFI 409 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 490 CTGCAG CTGCA^G
RsaI 696 GTAC GT^AC
SacII 263 CCGCGG CCGC^GG
SfcI 490 CTGCAG C^T[GA][CT]AG
SmlI 227 CTCGAG C^T[CT][GA]AG
TfiI 517 GAATC G^A[AT]TC
TspRI 53 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 409 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 584 CCATGGTGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 227 CTCGAG C^TCGAG
XhoII 68 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 448 GTCTAC GT^[AC][GT]AC
475 GTCTAC
AlwI 69 GATCC GGATC(4/5)
308 GATCC
BanII 179 GGGCTC G[GA]GC[CT]^C
268 GGGCCC
BbvI 125 GCAGC GCAGC(8/12)
21 GCTGC
BseRI 10 CTCCTC GAGGAG(10/8)
248 GAGGAG
BspMI 430 ACCTGC ACCTGC(4/8)
621 GCAGGT
BsrFI 40 GCCGGC [GA]^CCGG[CT]
418 ACCGGC
Cfr10I 40 GCCGGC [GA]^CCGG[CT]
418 ACCGGC
EagI 38 CGGCCG C^GGCCG
159 CGGCCG
EclXI 38 CGGCCG C^GGCCG
159 CGGCCG
Eco52I 38 CGGCCG C^GGCCG
159 CGGCCG
EcoT14I 120 CCATGG C^C[AT][AT]GG
584 CCATGG
HaeII 319 AGCGCC [GA]GCGC^[CT]
326 AGCGCT
HgaI 358 GACGC GACGC(5/10)
409 GACGC
MaeIII 415 GTCAC ^GT[ACGT]AC
635 GTGAC
MboII 507 GAAGA GAAGA(8/7)
533 TCTTC
NcoI 120 CCATGG C^CATGG
584 CCATGG
SfaNI 647 GCATC GCATC(5/9)
659 GCATC
StyI 120 CCATGG C^C[AT][AT]GG
584 CCATGG
TaqI 228 TCGA T^CGA
374 TCGA
TseI 21 GCTGC G^C[AT]GC
125 GCAGC
Tsp45I 415 GTCAC ^GT[GC]AC
635 GTGAC
TthHB8I 228 TCGA T^CGA
374 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AvaI AvaII BanI
BcnI BglI BmyI BsaJI BsiEI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MboI MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PpuMI Sau3AI Sau96I
ScrFI SfiI SmaI XmaCI XmaI