Restriction map for
pFN21AE4345
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AspHI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KspI MaeII MamI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tsp45I Tth111I
TthHB8I XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 70 GGTACC G^GTACC
AccII 64 CGCG CG^CG
AcsI 382 AAATTT [GA]^AATT[CT]
AlwNI 610 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 976 GGGCCC GGGCC^C
ApoI 382 AAATTT [GA]^AATT[CT]
Asp718I 70 GGTACC G^GTACC
AvaI 721 CCCGAG C^[CT]CG[GA]G
BalI 1013 TGGCCA TGG^CCA
BanII 976 GGGCCC G[GA]GC[CT]^C
BbsI 393 GAAGAC GAAGAC(2/6)
BlpI 1026 GCTGAGC GC^T[ACGT]AGC
BmyI 976 GGGCCC G[AGT]GC[ACT]^C
Bpu1102I 1026 GCTGAGC GC^T[ACGT]AGC
BpuAI 393 GAAGAC GAAGAC(2/6)
BsaI 877 GAGACC GGTCTC(1/5)
BseRI 839 GAGGAG GAGGAG(10/8)
BsmI 628 GAATGC GAATGC(1/-1)
BsoBI 721 CCCGAG C^[CT]CG[GA]G
Bsp1286I 976 GGGCCC G[AGT]GC[ACT]^C
BspHI 357 TCATGA T^CATGA
BspMI 296 ACCTGC ACCTGC(4/8)
BssSI 971 CACGAG CACGAG(-5/-1)
BstAPI 609 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 64 CGCG CG^CG
BstXI 554 CCATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1026 GCTGAGC GC^T[ACGT]AGC
CfoI 582 GCGC GCG^C
EaeI 1013 TGGCCA [CT]^GGCC[GA]
EcoNI 755 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 630 ATGCAT ATGCA^T
HhaI 582 GCGC GCG^C
HinP1I 582 GCGC G^CGC
KpnI 70 GGTACC GGTAC^C
MluNI 1013 TGGCCA TGG^CCA
MscI 1013 TGGCCA TGG^CCA
MseI 1070 TTAA T^TAA
MvnI 64 CGCG CG^CG
NsiI 630 ATGCAT ATGCA^T
PflMI 993 CCAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 293 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 357 TCATGA T^CATGA
SapI 446 GAAGAGC GCTCTTC(1/4)
SexAI 272 ACCTGGT A^CC[AT]GGT
Tru9I 1070 TTAA T^TAA
Tsp509I 383 AATT ^AATT
Van91I 993 CCAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 852 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 24 enzymes) #
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BanI 70 GGTACC G^G[CT][GA]CC
764 GGCACC
BcnI 515 CCCGG CC^[GC]GG
600 CCGGG
BfaI 400 CTAG C^TAG
853 CTAG
BsmAI 724 GAGAC GTCTC(1/5)
877 GAGAC
BsmFI 179 GGGAC GGGAC(10/14)
901 GTCCC
BsrDI 159 CATTGC GCAATG(2/0)
14 GCAATG
BstYI 611 AGATCC [GA]^GATC[CT]
696 GGATCT
EarI 417 GAAGAG CTCTTC(1/4)
446 GAAGAG
HapII 516 CCGG C^CGG
600 CCGG
HpaII 516 CCGG C^CGG
600 CCGG
HphI 323 TCACC GGTGA(8/7)
375 TCACC
Ksp632I 417 GAAGAG CTCTTC(1/4)
446 GAAGAG
MaeI 400 CTAG C^TAG
853 CTAG
MaeIII 8 GTAAC ^GT[ACGT]AC
962 GTAAC
MflI 611 AGATCC [GA]^GATC[CT]
696 GGATCT
MslI 159 CATTGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
913 CATGAAGATG
MspA1I 457 CAGCTG C[AC]G^C[GT]G
569 CAGCTG
MspI 516 CCGG C^CGG
600 CCGG
NciI 515 CCCGG CC^[GC]GG
600 CCGGG
NspI 246 ACATGC [GA]CATG^[CT]
632 GCATGT
PvuII 457 CAGCTG CAG^CTG
569 CAGCTG
TfiI 60 GATTC G^A[AT]TC
186 GATTC
XcmI 909 CCAGCATGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1040 CCAAAAACATCCTGG
XhoII 611 AGATCC [GA]^GATC[CT]
696 GGATCT
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AlwI AocI
AvaII BbvI BpmI BsaJI BsgI
BsiYI BslI BsrI BssKI BstF5I
BstNI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HinfI ItaI MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
TspRI