Restriction map for
pFN21AE9562
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwNI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BanI BbeI BbrPI BbvCI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsiEI BsiWI BsmAI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KasI KpnI Ksp632I
MamI MfeI MluI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 160 GACGTC GACGT^C
AccIII 212 TCCGGA T^CCGGA
AcyI 160 GACGTC G[GA]^CG[CT]C
AocI 856 CCTGAGG CC^T[ACGT]AGG
Aor51HI 123 AGCGCT AGC^GCT
ApaI 317 GGGCCC GGGCC^C
AvaI 57 CCCGGG C^[CT]CG[GA]G
AviII 662 TGCGCA TGC^GCA
BamHI 962 GGATCC G^GATCC
BbsI 382 GTCTTC GAAGAC(2/6)
BcgI 806 GCATCGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 875 CTAG C^TAG
BpuAI 382 GTCTTC GAAGAC(2/6)
BsaHI 160 GACGTC G[GA]^CG[CT]C
BsaWI 212 TCCGGA [AT]^CCGG[AT]
BseAI 212 TCCGGA T^CCGGA
BsmFI 537 GTCCC GGGAC(10/14)
BsoBI 57 CCCGGG C^[CT]CG[GA]G
BspEI 212 TCCGGA T^CCGGA
BsrFI 455 ACCGGC [GA]^CCGG[CT]
BssHII 80 GCGCGC G^CGCGC
BssSI 292 CTCGTG CACGAG(-5/-1)
BstAPI 713 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 856 CCTGAGG CC^T[ACGT]AGG
Cfr10I 455 ACCGGC [GA]^CCGG[CT]
Eco47III 123 AGCGCT AGC^GCT
Eco81I 856 CCTGAGG CC^T[ACGT]AGG
EcoNI 321 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1010 CCAAGG C^C[AT][AT]GG
FspI 662 TGCGCA TGC^GCA
HgaI 368 GCGTC GACGC(5/10)
Hin1I 160 GACGTC G[GA]^CG[CT]C
KspI 131 CCGCGG CCGC^GG
MaeI 875 CTAG C^TAG
MaeII 161 ACGT A^CGT
MroI 212 TCCGGA T^CCGGA
PleI 240 GAGTC GAGTC(4/5)
PstI 1033 CTGCAG CTGCA^G
PvuII 900 CAGCTG CAG^CTG
SacII 131 CCGCGG CCGC^GG
SexAI 257 ACCTGGT A^CC[AT]GGT
SfcI 1033 CTGCAG C^T[GA][CT]AG
SmaI 57 CCCGGG CCC^GGG
StyI 1010 CCAAGG C^C[AT][AT]GG
TaqI 102 TCGA T^CGA
TfiI 449 GAATC G^A[AT]TC
Tsp509I 226 AATT ^AATT
TthHB8I 102 TCGA T^CGA
XcmI 521 CCAACTTCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 57 CCCGGG C^CCGGG
XmaI 57 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 546 GTAC GT^AC
597 GTAC
AvaII 95 GGACC G^G[AT]CC
536 GGTCC
BalI 296 TGGCCA TGG^CCA
821 TGGCCA
BcnI 57 CCCGG CC^[GC]GG
58 CCGGG
BsmI 909 GAATGC GAATGC(1/-1)
995 GCATTC
BspMI 659 ACCTGC ACCTGC(4/8)
220 GCAGGT
BsrI 885 ACTGG ACTGG(1/-1)
594 CCAGT
BstF5I 576 CATCC GGATG(2/0)
618 CATCC
BstXI 149 CCACCGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
755 CCATGTTTGTGG
BstYI 851 AGATCC [GA]^GATC[CT]
962 GGATCC
EaeI 296 TGGCCA [CT]^GGCC[GA]
821 TGGCCA
FokI 576 CATCC GGATG(9/13)
618 CATCC
HinfI 240 GAGTC G^A[ACGT]TC
449 GAATC
MaeIII 145 GTCAC ^GT[ACGT]AC
871 GTCAC
MboII 302 TCTTC GAAGA(8/7)
383 TCTTC
MflI 851 AGATCC [GA]^GATC[CT]
962 GGATCC
MluNI 296 TGGCCA TGG^CCA
821 TGGCCA
MscI 296 TGGCCA TGG^CCA
821 TGGCCA
MseI 407 TTAA T^TAA
800 TTAA
MspA1I 131 CCGCGG C[AC]G^C[GT]G
900 CAGCTG
NciI 57 CCCGG CC^[GC]GG
58 CCGGG
NlaIII 435 CATG CATG^
756 CATG
PpuMI 94 AGGACCC [GA]G^G[AT]CC[CT]
535 GGGTCCC
RsaI 546 GTAC GT^AC
597 GTAC
Tru9I 407 TTAA T^TAA
800 TTAA
Tsp45I 145 GTCAC ^GT[GC]AC
871 GTCAC
XhoII 851 AGATCC [GA]^GATC[CT]
962 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
BanII BbvI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
Bsp1286I BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MboI MnlI
MslI MspI MvaI MvnI MwoI
NdeII NlaIV PflMI Sau3AI Sau96I
ScrFI SfaNI TseI TspRI Van91I