Restriction map for
pFN21AE1135
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AfaI AflII AgeI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BamHI BbsI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI EarI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FspI HincII HindII HpaI KpnI
Ksp632I KspI MaeIII MamI MboII
MfeI MluI MroI MspA1I MunI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacII SalI SapI
ScaI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI SspBI SspI
SwaI Tsp45I Tsp509I Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 1018 GTAGAC GT^[AC][GT]AC
AccII 675 CGCG CG^CG
AflIII 480 ACACGT A^C[GA][CT]GT
AhdI 456 GACGCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 267 GGATC GGATC(4/5)
AocI 883 CCTGAGG CC^T[ACGT]AGG
AspEI 456 GACGCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 834 GAGCTC G[AT]GC[AT]^C
BalI 707 TGGCCA TGG^CCA
BbrPI 481 CACGTG CAC^GTG
BfaI 649 CTAG C^TAG
BglI 347 GCCACAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 481 CACGTG [CT]AC^GT[GA]
BseRI 895 CTCCTC GAGGAG(10/8)
BsgI 1009 CTGCAC GTGCAG(16/14)
BsiHKAI 834 GAGCTC G[AT]GC[AT]^C
BstUI 675 CGCG CG^CG
BstYI 267 GGATCT [GA]^GATC[CT]
Bsu36I 883 CCTGAGG CC^T[ACGT]AGG
DpnII 268 GATC ^GATC
DsaI 4 CCGTGG C^C[GA][CT]GG
Eam1105I 456 GACGCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 883 CCTGAGG CC^T[ACGT]AGG
EcoNI 879 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 998 CCAAGG C^C[AT][AT]GG
FseI 534 GGCCGGCC GGCCGG^CC
HindIII 796 AAGCTT A^AGCTT
MaeI 649 CTAG C^TAG
MboI 268 GATC ^GATC
MflI 267 GGATCT [GA]^GATC[CT]
MluNI 707 TGGCCA TGG^CCA
MscI 707 TGGCCA TGG^CCA
MseI 800 TTAA T^TAA
MslI 153 CATGCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 675 CGCG CG^CG
NdeII 268 GATC ^GATC
PmaCI 481 CACGTG CAC^GTG
PmlI 481 CACGTG CAC^GTG
PpuMI 770 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 834 GAGCTC GAGCT^C
Sau3AI 268 GATC ^GATC
SexAI 869 ACCTGGT A^CC[AT]GGT
SfaNI 293 GCATC GCATC(5/9)
StyI 998 CCAAGG C^C[AT][AT]GG
TaqI 101 TCGA T^CGA
TfiI 1030 GAATC G^A[AT]TC
Tru9I 800 TTAA T^TAA
TthHB8I 101 TCGA T^CGA
XcmI 992 CCAGCCCCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 267 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 735 GGCGCC G[GA]^CG[CT]C
950 GGCGCC
BbeI 735 GGCGCC GGCGC^C
950 GGCGCC
BbvCI 342 CCTCAGC CCTCAGC(-5/-2)
814 GCTGAGG
BsaHI 735 GGCGCC G[GA]^CG[CT]C
950 GGCGCC
BsaI 85 GAGACC GGTCTC(1/5)
435 GGTCTC
BspMI 281 ACCTGC ACCTGC(4/8)
768 GCAGGT
BsrI 803 ACTGG ACTGG(1/-1)
740 CCAGT
BssSI 528 CACGAG CACGAG(-5/-1)
514 CTCGTG
BstXI 164 CCATCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
200 CCAACCGGCTGG
HgaI 456 GACGC GACGC(5/10)
920 GCGTC
Hin1I 735 GGCGCC G[GA]^CG[CT]C
950 GGCGCC
KasI 735 GGCGCC G^GCGCC
950 GGCGCC
NaeI 535 GCCGGC GCC^GGC
947 GCCGGC
NarI 735 GGCGCC GG^CGCC
950 GGCGCC
NgoMIV 535 GCCGGC G^CCGGC
947 GCCGGC
SbfI 765 CCTGCAGG CCTGCA^GG
840 CCTGCAGG
SfoI 735 GGCGCC GGC^GCC
950 GGCGCC
SmaI 64 CCCGGG CCC^GGG
1025 CCCGGG
Sse8387I 765 CCTGCAGG CCTGCA^GG
840 CCTGCAGG
XmaCI 64 CCCGGG C^CCGGG
1025 CCCGGG
XmaI 64 CCCGGG C^CCGGG
1025 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AlwNI ApaI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrFI BssKI
BstF5I BstNI Cac8I CfoI Cfr10I
Cfr13I DdeI DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MnlI
MspI MvaI MwoI NciI NlaIII
NlaIV PleI PstI Sau96I ScrFI
SfcI StuI TseI TspRI