Restriction map for
pFN21AE1837
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
AocI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BbeI BbrPI BbsI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI Eam1105I Eco81I EcoO65I EcoRI
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 569 GTCTAC GT^[AC][GT]AC
Aor51HI 1046 AGCGCT AGC^GCT
ApaI 445 GGGCCC GGGCC^C
BamHI 544 GGATCC G^GATCC
BbvCI 480 CCTCAGC CCTCAGC(-5/-2)
BglI 438 GCCACCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmAI 591 GAGAC GTCTC(1/5)
BspHI 227 TCATGA T^CATGA
BsrBI 979 GAGCGG CCGCTC(-3/-3)
BsrFI 55 ACCGGC [GA]^CCGG[CT]
BsrI 1001 ACTGG ACTGG(1/-1)
BssHII 603 GCGCGC G^CGCGC
BstXI 779 CCAAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 544 GGATCC [GA]^GATC[CT]
Cfr10I 55 ACCGGC [GA]^CCGG[CT]
DdeI 481 CTCAG C^T[ACGT]AG
DpnII 545 GATC ^GATC
EarI 34 CTCTTC CTCTTC(1/4)
Eco47III 1046 AGCGCT AGC^GCT
EcoNI 1019 CCTGCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 790 GATATC GAT^ATC
Ksp632I 34 CTCTTC CTCTTC(1/4)
MaeII 1013 ACGT A^CGT
MaeIII 1015 GTCAC ^GT[ACGT]AC
MboI 545 GATC ^GATC
MflI 544 GGATCC [GA]^GATC[CT]
NdeII 545 GATC ^GATC
NotI 9 GCGGCCGC GC^GGCCGC
NspI 257 ACATGC [GA]CATG^[CT]
RcaI 227 TCATGA T^CATGA
SapI 33 GCTCTTC GCTCTTC(1/4)
Sau3AI 545 GATC ^GATC
SexAI 770 ACCTGGT A^CC[AT]GGT
SmlI 709 CTCAAG C^T[CT][GA]AG
StuI 233 AGGCCT AGG^CCT
TaqI 977 TCGA T^CGA
Tsp45I 1015 GTCAC ^GT[GC]AC
TthHB8I 977 TCGA T^CGA
XcmI 633 CCATGGGGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 544 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 996 GTAC GT^AC
1037 GTAC
Alw44I 208 GTGCAC G^TGCAC
742 GTGCAC
AlwI 545 GATCC GGATC(4/5)
544 GGATC
AlwNI 595 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
682 CAGGAGCTG
ApaLI 208 GTGCAC G^TGCAC
742 GTGCAC
AvaII 320 GGACC G^G[AT]CC
783 GGACC
BseRI 273 CTCCTC GAGGAG(10/8)
493 CTCCTC
BsiEI 10 CGGCCG CG[GA][CT]^CG
353 CGGCCG
BstAPI 681 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
768 GCACCTGGTGC
DraII 319 GGGACCC [GA]G^G[ACGT]CC[CT]
782 AGGACCT
DraIII 199 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
769 CACCTGGTG
DrdI 321 GACCCGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
967 GACCGCGTGGTC
EagI 10 CGGCCG C^GGCCG
353 CGGCCG
EclXI 10 CGGCCG C^GGCCG
353 CGGCCG
Eco52I 10 CGGCCG C^GGCCG
353 CGGCCG
EcoO109I 319 GGGACCC [GA]G^G[ACGT]CC[CT]
782 AGGACCT
HgaI 123 GACGC GACGC(5/10)
567 GCGTC
HinfI 328 GAGTC G^A[ACGT]TC
808 GACTC
MboII 852 GAAGA GAAGA(8/7)
35 TCTTC
MslI 201 CACGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
574 CACAACAGTG
NcoI 633 CCATGG C^CATGG
752 CCATGG
PleI 808 GACTC GAGTC(4/5)
328 GAGTC
PpuMI 319 GGGACCC [GA]G^G[AT]CC[CT]
782 AGGACCT
RsaI 996 GTAC GT^AC
1037 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BsgI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DsaI EaeI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PstI PvuII SacI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TseI TspRI XmaCI XmaI