Restriction map for
pFN21AE2293
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwI AlwNI AocI ApoI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbvCI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BsrI BssSI Bst1107I BstBI BstF5I
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DpnII DraI DraIII
DrdI Eam1105I EarI Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FokI
FseI FspI HgaI HincII HindII
HindIII HpaI KpnI Ksp632I MaeI
MamI MboI MfeI MflI MluI
MroI MseI MslI MunI NcoI
NdeI NdeII NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SalI
SapI Sau3AI SbfI ScaI SexAI
SfcI SfiI SfuI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tsp509I
Tth111I TthHB8I Van91I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AfaI 519 GTAC GT^AC
Alw44I 462 GTGCAC G^TGCAC
Aor51HI 27 AGCGCT AGC^GCT
ApaI 7 GGGCCC GGGCC^C
ApaLI 462 GTGCAC G^TGCAC
AscI 165 GGCGCGCC GG^CGCGCC
AvaI 10 CCCGGG C^[CT]CG[GA]G
AvaII 42 GGTCC G^G[AT]CC
BbsI 555 GAAGAC GAAGAC(2/6)
BcgI 527 GCATGTTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 555 GAAGAC GAAGAC(2/6)
BsgI 98 CTGCAC GTGCAG(16/14)
BsmFI 13 GGGAC GGGAC(10/14)
BsoBI 10 CCCGGG C^[CT]CG[GA]G
BspMI 227 ACCTGC ACCTGC(4/8)
BstAPI 91 GCACCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 594 GGTCACC G^GT[ACGT]ACC
BstPI 594 GGTCACC G^GT[ACGT]ACC
DdeI 541 CTGAG C^T[ACGT]AG
DraII 6 GGGGCCC [GA]G^G[ACGT]CC[CT]
Eco47III 27 AGCGCT AGC^GCT
EcoO109I 6 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 594 GGTCACC G^GT[ACGT]ACC
EcoT14I 191 CCAAGG C^C[AT][AT]GG
KspI 20 CCGCGG CCGC^GG
MaeIII 595 GTCAC ^GT[ACGT]AC
NaeI 217 GCCGGC GCC^GGC
NgoMIV 217 GCCGGC G^CCGGC
NspI 527 GCATGT [GA]CATG^[CT]
PleI 508 GAGTC GAGTC(4/5)
RsaI 519 GTAC GT^AC
SacI 349 GAGCTC GAGCT^C
SacII 20 CCGCGG CCGC^GG
SgrAI 216 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 10 CCCGGG CCC^GGG
StyI 191 CCAAGG C^C[AT][AT]GG
TfiI 477 GATTC G^A[AT]TC
Tsp45I 595 GTCAC ^GT[GC]AC
TspRI 96 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 104 CCAACGGCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 10 CCCGGG C^CCGGG
XmaI 10 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AspHI 349 GAGCTC G[AT]GC[AT]^C
462 GTGCAC
BalI 392 TGGCCA TGG^CCA
410 TGGCCA
BanII 7 GGGCCC G[GA]GC[CT]^C
349 GAGCTC
BsiHKAI 349 GAGCTC G[AT]GC[AT]^C
462 GTGCAC
BsrBI 210 GAGCGG CCGCTC(-3/-3)
364 GAGCGG
BssHII 166 GCGCGC G^CGCGC
317 GCGCGC
DsaI 20 CCGCGG C^C[GA][CT]GG
359 CCGTGG
HinfI 477 GATTC G^A[ACGT]TC
508 GAGTC
MaeII 266 ACGT A^CGT
466 ACGT
MluNI 392 TGGCCA TGG^CCA
410 TGGCCA
MscI 392 TGGCCA TGG^CCA
410 TGGCCA
MspA1I 20 CCGCGG C[AC]G^C[GT]G
135 CCGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AcyI AluI BanI
BbeI BbvI BcnI BglI BmyI
BpmI BsaHI BsaJI BsiEI BsiYI
BslI Bsp1286I BsrFI BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
EaeI EagI EclXI Eco52I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HhaI Hin1I HinP1I HpaII HphI
ItaI KasI MboII MnlI MspI
MvaI MvnI MwoI NarI NciI
NlaIII NlaIV Sau96I ScrFI SfaNI
SfoI TseI