Restriction map for
pFN21AE9406
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# NO CUT ENZYMES ( 108 enzymes) #
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AclI AcsI AflII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AviII
AvrII BbeI BcgI BciVI BclI
BfrI BglII BlnI BsaBI BsaI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
DraI DraIII Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HincII HindII HpaI
KasI KspI MamI MfeI MluI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PstI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp509I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 796 GACGTC GACGT^C
Acc65I 413 GGTACC G^GTACC
AccII 939 CGCG CG^CG
AccIII 905 TCCGGA T^CCGGA
AcyI 796 GACGTC G[GA]^CG[CT]C
AflIII 190 ACACGT A^C[GA][CT]GT
ApaI 1319 GGGCCC GGGCC^C
Asp718I 413 GGTACC G^GTACC
BanII 1319 GGGCCC G[GA]GC[CT]^C
BbrPI 634 CACGTG CAC^GTG
BglI 706 GCCCGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 634 CACGTG [CT]AC^GT[GA]
BsaHI 796 GACGTC G[GA]^CG[CT]C
BsaWI 905 TCCGGA [AT]^CCGG[AT]
BseAI 905 TCCGGA T^CCGGA
BsgI 1244 CTGCAC GTGCAG(16/14)
BspEI 905 TCCGGA T^CCGGA
BsrBI 958 GAGCGG CCGCTC(-3/-3)
BstAPI 1246 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 939 CGCG CG^CG
DsaI 11 CCATGG C^C[GA][CT]GG
EagI 961 CGGCCG C^GGCCG
EclXI 961 CGGCCG C^GGCCG
Eco52I 961 CGGCCG C^GGCCG
EcoNI 1309 CCTACCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 796 GACGTC G[GA]^CG[CT]C
HindIII 1283 AAGCTT A^AGCTT
KpnI 413 GGTACC GGTAC^C
MaeIII 1047 GTCAC ^GT[ACGT]AC
MroI 905 TCCGGA T^CCGGA
MslI 6 CACTACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 939 CGCG CG^CG
NaeI 160 GCCGGC GCC^GGC
NcoI 11 CCATGG C^CATGG
NgoMIV 160 GCCGGC G^CCGGC
PflMI 133 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 70 GACTC GAGTC(4/5)
PmaCI 634 CACGTG CAC^GTG
PmlI 634 CACGTG CAC^GTG
PvuI 1099 CGATCG CGAT^CG
SexAI 533 ACCTGGT A^CC[AT]GGT
SgrAI 456 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 1392 CCCGGG CCC^GGG
SmlI 1324 CTTGAG C^T[CT][GA]AG
TaqI 380 TCGA T^CGA
Tsp45I 1047 GTCAC ^GT[GC]AC
TthHB8I 380 TCGA T^CGA
Van91I 133 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1392 CCCGGG C^CCGGG
XmaI 1392 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 799 GTCTAC GT^[AC][GT]AC
832 GTCTAC
AfaI 414 GTAC GT^AC
528 GTAC
AlwNI 1199 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
1269 CAGTCCCTG
AocI 744 CCTGAGG CC^T[ACGT]AGG
1072 CCTCAGG
AspHI 637 GTGCTC G[AT]GC[AT]^C
925 GTGCTC
AspI 94 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
532 GACCTGGTC
AvaI 508 CCCGAG C^[CT]CG[GA]G
1392 CCCGGG
BbsI 100 GTCTTC GAAGAC(2/6)
502 GTCTTC
BfaI 265 CTAG C^TAG
1000 CTAG
BlpI 210 GCTCAGC GC^T[ACGT]AGC
1114 GCTCAGC
BpmI 1090 CTCCAG CTGGAG(16/14)
586 CTGGAG
Bpu1102I 210 GCTCAGC GC^T[ACGT]AGC
1114 GCTCAGC
BpuAI 100 GTCTTC GAAGAC(2/6)
502 GTCTTC
BsiHKAI 637 GTGCTC G[AT]GC[AT]^C
925 GTGCTC
BsoBI 508 CCCGAG C^[CT]CG[GA]G
1392 CCCGGG
BsrFI 160 GCCGGC [GA]^CCGG[CT]
457 GCCGGT
Bsu36I 744 CCTGAGG CC^T[ACGT]AGG
1072 CCTCAGG
CelII 210 GCTCAGC GC^T[ACGT]AGC
1114 GCTCAGC
Cfr10I 160 GCCGGC [GA]^CCGG[CT]
457 GCCGGT
DrdI 720 GACCATTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1225 GACACTAACGTC
EarI 355 CTCTTC CTCTTC(1/4)
481 CTCTTC
Eco81I 744 CCTGAGG CC^T[ACGT]AGG
1072 CCTCAGG
HaeII 198 GGCGCT [GA]GCGC^[CT]
396 GGCGCT
HphI 1077 GGTGA GGTGA(8/7)
641 TCACC
Ksp632I 355 CTCTTC CTCTTC(1/4)
481 CTCTTC
MaeI 265 CTAG C^TAG
1000 CTAG
PflFI 94 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
532 GACCTGGTC
PpuMI 590 AGGTCCT [GA]G^G[AT]CC[CT]
1328 AGGACCC
RsaI 414 GTAC GT^AC
528 GTAC
SfaNI 582 GATGC GCATC(5/9)
1305 GCATC
TfiI 1003 GATTC G^A[AT]TC
1201 GATTC
Tth111I 94 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
532 GACCTGGTC
XcmI 137 CCAATGGGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
168 CCAGGCCCGGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AluI AlwI AvaII BalI
BamHI BanI BbvCI BbvI BcnI
BmyI BsaJI BseRI BsiEI BsiYI
BslI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PvuII
Sau3AI Sau96I ScrFI StyI TseI
TspRI XhoII