##################################
# NO CUT ENZYMES (  165 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AvaI        AviII       AvrII       
BalI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BcnI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmAI       BsmBI       BsoBI       BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrDI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FseI        FspI        HaeII       HapII       
HhaI        HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MaeII       MaeIII      MfeI        MluI        MluNI       
MroI        MscI        MseI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        Tru9I       Tsp45I      
Tsp509I     XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AcyI          164  GACGCC               G[GA]^CG[CT]C
AspHI         132  GAGCTC               G[AT]GC[AT]^C
BamHI         488  GGATCC               G^GATCC
BanI          612  GGCACC               G^G[CT][GA]CC
BclI          386  TGATCA               T^GATCA
BsaBI         657  GATCGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         164  GACGCC               G[GA]^CG[CT]C
BsaJI         312  CCTTGG               C^C[ACGT][ACGT]GG
BsgI          553  CTGCAC               GTGCAG(16/14)
BsiHKAI       132  GAGCTC               G[AT]GC[AT]^C
BsmI          344  GCATTC               GAATGC(1/-1)
Bsp1407I      641  TGTACA               T^GTACA
BsrGI         641  TGTACA               T^GTACA
BsrI          674  ACTGG                ACTGG(1/-1)
Cac8I         555  GCACGC               GC[ACGT]^[ACGT]GC
EcoT14I       312  CCTTGG               C^C[AT][AT]GG
FbaI          386  TGATCA               T^GATCA
HaeIII        628  GGCC                 GG^CC
HgaI          164  GACGC                GACGC(5/10)
Hin1I         164  GACGCC               G[GA]^CG[CT]C
HphI           49  GGTGA                GGTGA(8/7)
MamI          657  GATCGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI          345  CATTCAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII        645  CATG                 CATG^
NspI          644  ACATGC               [GA]CATG^[CT]
SacI          132  GAGCTC               GAGCT^C
SfaNI         189  GATGC                GCATC(5/9)
SmlI          334  CTCAAG               C^T[CT][GA]AG
SspBI         641  TGTACA               T^GTACA
StyI          312  CCTTGG               C^C[AT][AT]GG
TaqI          659  TCGA                 T^CGA
TspRI         183  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       659  TCGA                 T^CGA
XcmI          422  CCAAACAGCACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AfaI          399  GTAC                 GT^AC
              642  GTAC                
Asp700I       204  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              228  GAAGTTGTTC          
AspI          114  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              245  GACTGAGTC           
BfaI          199  CTAG                 C^TAG
              695  CTAG                
MaeI          199  CTAG                 C^TAG
              695  CTAG                
PflFI         114  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              245  GACTGAGTC           
PvuII         253  CAGCTG               CAG^CTG
              701  CAGCTG              
RsaI          399  GTAC                 GT^AC
              642  GTAC                
TfiI           52  GATTC                G^A[AT]TC
              339  GATTC               
Tth111I       114  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              245  GACTGAGTC           
XmnI          204  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              228  GAAGTTGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       AvaII       
BanII       BbvI        BmyI        BpmI        BseRI       
BsiYI       BslI        BsmFI       Bsp1286I    BsrBI       
BssKI       BstF5I      BstNI       BstYI       Cfr13I      
DdeI        DpnII       EarI        EcoRII      Fnu4HI      
FokI        HinfI       ItaI        Ksp632I     MboI        
MboII       MflI        MnlI        MspA1I      MvaI        
MwoI        NdeII       NlaIV       PflMI       PleI        
PstI        Sau3AI      Sau96I      ScrFI       SfcI        
TseI        Van91I      XhoII