Restriction map for
pFN21AE5174
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII AccII AclI AcsI AcyI
AflII AflIII AhdI Alw44I AlwNI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaHI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
Ksp632I KspI MfeI MluI MluNI
MscI MslI MunI MvnI MwoI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TfiI Tsp509I
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 433 GGTACC G^GTACC
AccI 286 GTCTAC GT^[AC][GT]AC
AccIII 329 TCCGGA T^CCGGA
AgeI 43 ACCGGT A^CCGGT
Asp718I 433 GGTACC G^GTACC
BamHI 167 GGATCC G^GATCC
BanI 433 GGTACC G^G[CT][GA]CC
BbsI 399 GAAGAC GAAGAC(2/6)
BcnI 18 CCGGG CC^[GC]GG
BfaI 206 CTAG C^TAG
BpmI 95 CTCCAG CTGGAG(16/14)
BpuAI 399 GAAGAC GAAGAC(2/6)
BsaBI 127 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 227 GAGACC GGTCTC(1/5)
BseAI 329 TCCGGA T^CCGGA
BsmAI 227 GAGAC GTCTC(1/5)
BsmFI 344 GGGAC GGGAC(10/14)
BspEI 329 TCCGGA T^CCGGA
BspMI 242 ACCTGC ACCTGC(4/8)
BstXI 107 CCAGGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 147 GCGC GCG^C
DdeI 382 CTGAG C^T[ACGT]AG
HaeII 146 AGCGCC [GA]GCGC^[CT]
HgaI 260 GACGC GACGC(5/10)
HhaI 147 GCGC GCG^C
HinP1I 147 GCGC G^CGC
HinfI 118 GAGTC G^A[ACGT]TC
KpnI 433 GGTACC GGTAC^C
MaeI 206 CTAG C^TAG
MaeII 368 ACGT A^CGT
MamI 127 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 329 TCCGGA T^CCGGA
MseI 470 TTAA T^TAA
NciI 18 CCGGG CC^[GC]GG
PinAI 43 ACCGGT A^CCGGT
PleI 118 GAGTC GAGTC(4/5)
PshAI 402 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 358 CTGCAG CTGCA^G
SfaNI 270 GATGC GCATC(5/9)
SfcI 358 CTGCAG C^T[GA][CT]AG
Tru9I 470 TTAA T^TAA
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AfaI 256 GTAC GT^AC
434 GTAC
AluI 6 AGCT AG^CT
467 AGCT
ApaI 90 GGGCCC GGGCC^C
353 GGGCCC
Asp700I 373 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
489 GAAAGGCTTC
BsaWI 43 ACCGGT [AT]^CCGG[AT]
329 TCCGGA
BstYI 167 GGATCC [GA]^GATC[CT]
174 AGATCC
HaeIII 91 GGCC GG^CC
354 GGCC
HphI 426 GGTGA GGTGA(8/7)
410 TCACC
MboII 373 GAAGA GAAGA(8/7)
399 GAAGA
MflI 167 GGATCC [GA]^GATC[CT]
174 AGATCC
MspA1I 52 CAGCGG C[AC]G^C[GT]G
388 CCGCTG
RsaI 256 GTAC GT^AC
434 GTAC
Tsp45I 409 GTCAC ^GT[GC]AC
427 GTGAC
XhoII 167 GGATCC [GA]^GATC[CT]
174 AGATCC
XmnI 373 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
489 GAAAGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AlwI BanII BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BsrFI
BssKI BstF5I BstNI Cac8I Cfr10I
Cfr13I DpnII DraII EcoO109I EcoRII
FauI Fnu4HI FokI HapII HpaII
ItaI MaeIII MboI MnlI MspI
MvaI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TseI