##################################
# NO CUT ENZYMES (  197 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DdeI        DraI        DraII       
DraIII      DrdI        DsaI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FokI        FseI        FspI        
HaeII       HincII      HindII      HinfI       HpaI        
HphI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MamI        MboII       MfeI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        TfiI        Tru9I       Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

AcyI           12  GACGCC               G[GA]^CG[CT]C
AluI          101  AGCT                 AG^CT
BamHI         170  GGATCC               G^GATCC
BcnI           47  CCCGG                CC^[GC]GG
BpmI          184  CTGGAG               CTGGAG(16/14)
BsaHI          12  GACGCC               G[GA]^CG[CT]C
BsaI          187  GAGACC               GGTCTC(1/5)
BsmAI         187  GAGAC                GTCTC(1/5)
BsrI           58  CCAGT                ACTGG(1/-1)
BssHII         66  GCGCGC               G^CGCGC
BstNI         183  CCTGG                CC^[AT]GG
BstXI         160  CCAGCGGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I         45  GGCCC                G^G[ACGT]CC
EaeI          119  TGGCCG               [CT]^GGCC[GA]
EcoNI         191  CCTACAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII        183  CCTGG                ^CC[AT]GG
HgaI           12  GACGC                GACGC(5/10)
Hin1I          12  GACGCC               G[GA]^CG[CT]C
HindIII       100  AAGCTT               A^AGCTT
MvaI          183  CCTGG                CC^[AT]GG
NciI           47  CCCGG                CC^[GC]GG
NlaIII        111  CATG                 CATG^
Sau96I         45  GGCCC                G^G[ACGT]CC
SfcI          192  CTACAG               C^T[GA][CT]AG
Tsp509I       116  AATT                 ^AATT
TspRI          59  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccII          65  CGCG                 CG^CG
               67  CGCG                
BssKI          47  CCCGG                ^CC[ACGT]GG
              183  CCTGG               
BstUI          65  CGCG                 CG^CG
               67  CGCG                
BstYI          74  AGATCC               [GA]^GATC[CT]
              170  GGATCC              
CfoI           66  GCGC                 GCG^C
               68  GCGC                
FauI           27  GCGGG                CCCGC(4/6)
              163  GCGGG               
HapII          48  CCGG                 C^CGG
               91  CCGG                
HhaI           66  GCGC                 GCG^C
               68  GCGC                
HinP1I         66  GCGC                 G^CGC
               68  GCGC                
HpaII          48  CCGG                 C^CGG
               91  CCGG                
MaeIII        145  GTGAC                ^GT[ACGT]AC
              177  GTCAC               
MflI           74  AGATCC               [GA]^GATC[CT]
              170  GGATCC              
MspA1I         16  CCGCTG               C[AC]G^C[GT]G
              161  CAGCGG              
MspI           48  CCGG                 C^CGG
               91  CCGG                
MvnI           65  CGCG                 CG^CG
               67  CGCG                
NlaIV          53  GGCTCC               GG[ACGT]^[ACGT]CC
              170  GGATCC              
ScrFI          47  CCCGG                CC^[ACGT]GG
              183  CCTGG               
Tsp45I        145  GTGAC                ^GT[GC]AC
              177  GTCAC               
XhoII          74  AGATCC               [GA]^GATC[CT]
              170  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   16 enzymes) #
#########################################

AciI        AlwI        BbvI        BsiYI       BslI        
Cac8I       DpnII       Fnu4HI      HaeIII      ItaI        
MboI        MnlI        MwoI        NdeII       Sau3AI      
TseI