Restriction map for
pFN21AE3034
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# NO CUT ENZYMES ( 191 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvaI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmBI BsmFI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssKI BssSI Bst1107I
BstBI BstEII BstF5I BstNI BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoRV EcoT22I FbaI FokI
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MfeI MluI MluNI MroI
MscI MslI MspI MunI MvaI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI ScrFI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tsp509I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 9 GTCTAC GT^[AC][GT]AC
AfaI 477 GTAC GT^AC
AlwNI 451 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 24 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 200 GAGCCC G[GA]GC[CT]^C
BfaI 335 CTAG C^TAG
BglI 156 GCCTCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 468 AGATCT A^GATCT
BsaBI 141 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 160 CCATGG C^C[ACGT][ACGT]GG
BsmI 248 GCATTC GAATGC(1/-1)
BstAPI 450 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 468 AGATCT [GA]^GATC[CT]
DpnII 469 GATC ^GATC
DsaI 160 CCATGG C^C[GA][CT]GG
EcoT14I 160 CCATGG C^C[AT][AT]GG
FauI 203 CCCGC CCCGC(4/6)
HgaI 95 GACGC GACGC(5/10)
HphI 109 GGTGA GGTGA(8/7)
MaeI 335 CTAG C^TAG
MaeII 495 ACGT A^CGT
MamI 141 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 469 GATC ^GATC
MboII 252 TCTTC GAAGA(8/7)
MflI 468 AGATCT [GA]^GATC[CT]
MseI 326 TTAA T^TAA
MspA1I 301 CAGCTG C[AC]G^C[GT]G
NcoI 160 CCATGG C^CATGG
NdeII 469 GATC ^GATC
PflFI 24 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 280 CTGCAG CTGCA^G
PvuII 301 CAGCTG CAG^CTG
RsaI 477 GTAC GT^AC
Sau3AI 469 GATC ^GATC
SfaNI 36 GATGC GCATC(5/9)
SmlI 472 CTTGAG C^T[CT][GA]AG
SspI 390 AATATT AAT^ATT
StyI 160 CCATGG C^C[AT][AT]GG
TaqI 22 TCGA T^CGA
Tru9I 326 TTAA T^TAA
Tth111I 24 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 22 TCGA T^CGA
XhoII 468 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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AluI 302 AGCT AG^CT
372 AGCT
BbvI 282 GCAGC GCAGC(8/12)
300 GCAGC
BmyI 200 GAGCCC G[AGT]GC[ACT]^C
461 GTGCCC
Bsp1286I 200 GAGCCC G[AGT]GC[ACT]^C
461 GTGCCC
BsrI 344 ACTGG ACTGG(1/-1)
359 ACTGG
Cfr13I 362 GGGCC G^G[ACGT]CC
411 GGCCC
Fnu4HI 282 GCAGC GC^[ACGT]GC
300 GCAGC
HaeIII 363 GGCC GG^CC
411 GGCC
HinfI 124 GATTC G^A[ACGT]TC
181 GAATC
ItaI 282 GCAGC GC^[ACGT]GC
300 GCAGC
MaeIII 82 GTGAC ^GT[ACGT]AC
110 GTGAC
Sau96I 362 GGGCC G^G[ACGT]CC
411 GGCCC
TfiI 124 GATTC G^A[AT]TC
181 GAATC
TseI 282 GCAGC G^C[AT]GC
300 GCAGC
Tsp45I 82 GTGAC ^GT[GC]AC
110 GTGAC
TspRI 14 CAGTG CA[GC]TG[ACGT][ACGT]^
78 CAGTG
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
#########################################
AciI BsmAI Cac8I DdeI MnlI
MwoI NlaIII SfcI