##################################
# NO CUT ENZYMES (  206 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsmBI       
BsmFI       BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DpnII       DraI        DraII       DraIII      
DrdI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FokI        FseI        FspI        
HaeII       HaeIII      HgaI        HhaI        Hin1I       
HinP1I      HindIII     HinfI       HpaI        KasI        
KpnI        Ksp632I     KspI        MaeIII      MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MspA1I      MunI        MvnI        
NaeI        NarI        NdeI        NdeII       NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
Sau96I      SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        TfiI        
Tsp45I      Tsp509I     Tth111I     TthHB8I     Van91I      
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AccI          119  GTCTAC               GT^[AC][GT]AC
AluI          402  AGCT                 AG^CT
BcnI          222  CCCGG                CC^[GC]GG
BfaI          400  CTAG                 C^TAG
BglI          343  GCCAGTCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI           25  GAGACC               GGTCTC(1/5)
BsmI          170  GCATTC               GAATGC(1/-1)
BsrDI         245  CATTGC               GCAATG(2/0)
BsrI          344  CCAGT                ACTGG(1/-1)
DdeI          175  CTGAG                C^T[ACGT]AG
DsaI          320  CCATGG               C^C[GA][CT]GG
EcoT14I       320  CCATGG               C^C[AT][AT]GG
HapII         223  CCGG                 C^CGG
HincII        217  GTCAAC               GT[CT]^[GA]AC
HindII        217  GTCAAC               GT[CT]^[GA]AC
HpaII         223  CCGG                 C^CGG
HphI          310  TCACC                GGTGA(8/7)
MaeI          400  CTAG                 C^TAG
MaeII         117  ACGT                 A^CGT
MnlI          242  CCTC                 CCTC(7/6)
MseI          232  TTAA                 T^TAA
MspI          223  CCGG                 C^CGG
NciI          222  CCCGG                CC^[GC]GG
NcoI          320  CCATGG               C^CATGG
NlaIII        321  CATG                 CATG^
PstI          421  CTGCAG               CTGCA^G
SfaNI         369  GATGC                GCATC(5/9)
StyI          320  CCATGG               C^C[AT][AT]GG
Tru9I         232  TTAA                 T^TAA
XcmI          338  CCAAAGCCAGTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AspHI         179  GTGCTC               G[AT]GC[AT]^C
              406  GTGCTC              
BmyI          179  GTGCTC               G[AGT]GC[ACT]^C
              406  GTGCTC              
BsiHKAI       179  GTGCTC               G[AT]GC[AT]^C
              406  GTGCTC              
BsiYI          89  CCAACCAGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               93  CCAGGGGCAGG         
BslI           89  CCAACCAGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               93  CCAGGGGCAGG         
BsmAI          25  GAGAC                GTCTC(1/5)
              363  GTCTC               
Bsp1286I      179  GTGCTC               G[AGT]GC[ACT]^C
              406  GTGCTC              
MboII         111  GAAGA                GAAGA(8/7)
              288  TCTTC               
MwoI          181  GCTCTGTCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              343  GCCAGTCTGGC         
SfcI          121  CTACAG               C^T[GA][CT]AG
              421  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

BbvI        BsaJI       BssKI       BstNI       EcoRII      
Fnu4HI      ItaI        MslI        MvaI        ScrFI       
TseI        TspRI