Restriction map for
pFN21AB0932
##################################
# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AhdI Alw44I AlwI AocI ApaLI
AscI AseI AsnI Asp718I AspEI
AvaI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaJI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstNI BstUI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DpnII DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoRII EcoRV
EcoT14I FbaI FseI FspI HgaI
Hin1I HincII HindII HpaI KasI
KpnI Ksp632I KspI MaeII MamI
MboI MfeI MflI MluI MluNI
MroI MscI MunI MvaI MvnI
NaeI NarI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI XbaI
XcmI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AciI 383 CCGC CCGC(-3/-1)
AcsI 3 GAATTC [GA]^AATT[CT]
AgeI 362 ACCGGT A^CCGGT
Aor51HI 573 AGCGCT AGC^GCT
ApaI 586 GGGCCC GGGCC^C
ApoI 3 GAATTC [GA]^AATT[CT]
BbsI 22 GTCTTC GAAGAC(2/6)
BbvCI 397 GCTGAGG CCTCAGC(-5/-2)
BcnI 72 CCCGG CC^[GC]GG
BfaI 571 CTAG C^TAG
BpmI 345 CTCCAG CTGGAG(16/14)
BpuAI 22 GTCTTC GAAGAC(2/6)
BsaWI 362 ACCGGT [AT]^CCGG[AT]
BseRI 265 GAGGAG GAGGAG(10/8)
BsgI 547 CTGCAC GTGCAG(16/14)
BspMI 40 ACCTGC ACCTGC(4/8)
BsrFI 362 ACCGGT [GA]^CCGG[CT]
BssKI 72 CCCGG ^CC[ACGT]GG
BstEII 75 GGTCACC G^GT[ACGT]ACC
BstF5I 135 CATCC GGATG(2/0)
BstPI 75 GGTCACC G^GT[ACGT]ACC
CfoI 574 GCGC GCG^C
Cfr10I 362 ACCGGT [GA]^CCGG[CT]
Eco47III 573 AGCGCT AGC^GCT
EcoO65I 75 GGTCACC G^GT[ACGT]ACC
EcoRI 3 GAATTC G^AATTC
EcoT22I 162 ATGCAT ATGCA^T
FauI 382 CCCGC CCCGC(4/6)
FokI 135 CATCC GGATG(9/13)
HaeII 573 AGCGCT [GA]GCGC^[CT]
HhaI 574 GCGC GCG^C
HinP1I 574 GCGC G^CGC
HindIII 449 AAGCTT A^AGCTT
MaeI 571 CTAG C^TAG
MaeIII 76 GTCAC ^GT[ACGT]AC
MseI 421 TTAA T^TAA
MslI 48 CATCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 383 CCGCTG C[AC]G^C[GT]G
NciI 72 CCCGG CC^[GC]GG
NsiI 162 ATGCAT ATGCA^T
NspI 551 ACATGC [GA]CATG^[CT]
PflMI 239 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 362 ACCGGT A^CCGGT
PleI 535 GAGTC GAGTC(4/5)
SacI 258 GAGCTC GAGCT^C
ScrFI 72 CCCGG CC^[ACGT]GG
TaqI 204 TCGA T^CGA
Tru9I 421 TTAA T^TAA
Tsp45I 76 GTCAC ^GT[GC]AC
TthHB8I 204 TCGA T^CGA
Van91I 239 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Asp700I 57 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
300 GAAGATGTTC
AspI 70 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
374 GACACTGTC
BanII 258 GAGCTC G[GA]GC[CT]^C
586 GGGCCC
BsmFI 605 GGGAC GGGAC(10/14)
380 GTCCC
BsmI 160 GAATGC GAATGC(1/-1)
281 GCATTC
Cac8I 104 GCAGGC GC[ACGT]^[ACGT]GC
326 GCCTGC
DraII 106 AGGCCCT [GA]G^G[ACGT]CC[CT]
585 AGGGCCC
EcoO109I 106 AGGCCCT [GA]G^G[ACGT]CC[CT]
585 AGGGCCC
HaeIII 107 GGCC GG^CC
587 GGCC
HapII 73 CCGG C^CGG
363 CCGG
HpaII 73 CCGG C^CGG
363 CCGG
HphI 38 TCACC GGTGA(8/7)
77 TCACC
MspI 73 CCGG C^CGG
363 CCGG
NlaIII 54 CATG CATG^
552 CATG
NlaIV 323 GGAGCC GG[ACGT]^[ACGT]CC
586 GGGCCC
PflFI 70 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
374 GACACTGTC
Tth111I 70 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
374 GACACTGTC
XmnI 57 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
300 GAAGATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI AlwNI AspHI BbvI BmyI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI Cfr13I DdeI Fnu4HI HinfI
ItaI MboII MnlI MwoI Sau96I
TfiI TseI Tsp509I TspRI