Restriction map for
pFN21AE1801
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaHI
BsaWI BseAI BsiEI BsiWI BsiYI
BslI BsmBI BsmFI Bsp1407I BspDI
BspEI BspHI BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII KasI KpnI
KspI MaeIII MfeI MluI MluNI
MroI MscI MspA1I MunI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tsp45I
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AciI 672 GCGG CCGC(-3/-1)
AvaI 309 CTCGGG C^[CT]CG[GA]G
BbsI 573 GTCTTC GAAGAC(2/6)
BpuAI 573 GTCTTC GAAGAC(2/6)
BsaBI 49 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 903 GGTCTC GGTCTC(1/5)
BseRI 758 GAGGAG GAGGAG(10/8)
BsgI 727 CTGCAC GTGCAG(16/14)
BsmI 809 GAATGC GAATGC(1/-1)
BsoBI 309 CTCGGG C^[CT]CG[GA]G
BspLU11I 738 ACATGT A^CATGT
BsrBI 670 GAGCGG CCGCTC(-3/-3)
EaeI 456 TGGCCG [CT]^GGCC[GA]
EarI 712 CTCTTC CTCTTC(1/4)
EcoT14I 681 CCAAGG C^C[AT][AT]GG
EcoT22I 52 ATGCAT ATGCA^T
HapII 459 CCGG C^CGG
HpaI 129 GTTAAC GTT^AAC
HpaII 459 CCGG C^CGG
HphI 553 TCACC GGTGA(8/7)
Ksp632I 712 CTCTTC CTCTTC(1/4)
MaeII 382 ACGT A^CGT
MamI 49 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 717 CATTAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 459 CCGG C^CGG
NheI 72 GCTAGC G^CTAGC
NsiI 52 ATGCAT ATGCA^T
NspI 738 ACATGT [GA]CATG^[CT]
PstI 814 CTGCAG CTGCA^G
SfcI 814 CTGCAG C^T[GA][CT]AG
StyI 681 CCAAGG C^C[AT][AT]GG
XcmI 470 CCAGGTATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AfaI 25 GTAC GT^AC
590 GTAC
AflIII 382 ACGTGT A^C[GA][CT]GT
738 ACATGT
Alw44I 3 GTGCAC G^TGCAC
302 GTGCAC
ApaLI 3 GTGCAC G^TGCAC
302 GTGCAC
AspHI 3 GTGCAC G[AT]GC[AT]^C
302 GTGCAC
AvaII 408 GGTCC G^G[AT]CC
747 GGTCC
BanI 190 GGTGCC G^G[CT][GA]CC
356 GGTGCC
BanII 15 GAGCCC G[GA]GC[CT]^C
312 GGGCTC
BfaI 73 CTAG C^TAG
448 CTAG
BglII 114 AGATCT A^GATCT
818 AGATCT
BsaJI 308 CCTCGG C^C[ACGT][ACGT]GG
681 CCAAGG
BsiHKAI 3 GTGCAC G[AT]GC[AT]^C
302 GTGCAC
Cac8I 72 GCTAGC GC[ACGT]^[ACGT]GC
76 GCCAGC
DdeI 13 CTGAG C^T[ACGT]AG
923 CTAAG
HincII 129 GTTAAC GT[CT]^[GA]AC
203 GTCAAC
HindII 129 GTTAAC GT[CT]^[GA]AC
203 GTCAAC
MaeI 73 CTAG C^TAG
448 CTAG
MseI 130 TTAA T^TAA
935 TTAA
RsaI 25 GTAC GT^AC
590 GTAC
Tru9I 130 TTAA T^TAA
935 TTAA
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
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AcsI AluI AlwI ApoI BbvI
BmyI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cfr13I DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MboI MboII MflI MnlI
MvaI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TfiI TseI
Tsp509I TspRI XhoII