##################################
# NO CUT ENZYMES (  187 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BseRI       BsiEI       BsiWI       BsmBI       BsmFI       
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HaeII       HgaI        Hin1I       
HincII      HindII      HindIII     HinfI       HpaI        
HphI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeIII      MamI        MboII       MluI        
MluNI       MroI        MscI        MseI        MslI        
MspA1I      NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        TfiI        Tru9I       Tsp45I      Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccII         140  CGCG                 CG^CG
AfaI          266  GTAC                 GT^AC
AflIII        311  ACACGT               A^C[GA][CT]GT
AluI          194  AGCT                 AG^CT
AspHI         341  GAGCAC               G[AT]GC[AT]^C
AvaII          45  GGTCC                G^G[AT]CC
BanII         219  GGGCTC               G[GA]GC[CT]^C
BcnI          114  CCCGG                CC^[GC]GG
BglII         241  AGATCT               A^GATCT
BsaI          383  GAGACC               GGTCTC(1/5)
BsaJI          92  CCCTGG               C^C[ACGT][ACGT]GG
BsiHKAI       341  GAGCAC               G[AT]GC[AT]^C
BsmAI         383  GAGAC                GTCTC(1/5)
BsmI          414  GAATGC               GAATGC(1/-1)
BssHII        139  GCGCGC               G^CGCGC
BstUI         140  CGCG                 CG^CG
BstYI         241  AGATCT               [GA]^GATC[CT]
Cfr13I         45  GGTCC                G^G[ACGT]CC
DpnII         242  GATC                 ^GATC
EaeI          116  CGGCCA               [CT]^GGCC[GA]
EcoNI          48  CCTGTTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII         115  CCGG                 C^CGG
HpaII         115  CCGG                 C^CGG
MaeII         313  ACGT                 A^CGT
MboI          242  GATC                 ^GATC
MfeI          329  CAATTG               C^AATTG
MflI          241  AGATCT               [GA]^GATC[CT]
MspI          115  CCGG                 C^CGG
MunI          329  CAATTG               C^AATTG
MvnI          140  CGCG                 CG^CG
NciI          114  CCCGG                CC^[GC]GG
NdeII         242  GATC                 ^GATC
NlaIV         220  GGCTCC               GG[ACGT]^[ACGT]CC
RsaI          266  GTAC                 GT^AC
Sau3AI        242  GATC                 ^GATC
Sau96I         45  GGTCC                G^G[ACGT]CC
ScaI          265  AGTACT               AGT^ACT
SfaNI          38  GCATC                GCATC(5/9)
Tsp509I       330  AATT                 ^AATT
XcmI          321  CCAAACTGCAATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         241  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AciI          249  GCGG                 CCGC(-3/-1)
              254  GCGG                
BsrI          159  ACTGG                ACTGG(1/-1)
              188  CCAGT               
BstXI         188  CCAGTGAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              368  CCAGCGACTTGG        
CfoI          139  GCGC                 GCG^C
              141  GCGC                
HaeIII        117  GGCC                 GG^CC
              186  GGCC                
HhaI          139  GCGC                 GCG^C
              141  GCGC                
HinP1I        139  GCGC                 G^CGC
              141  GCGC                
NspI          272  GCATGC               [GA]CATG^[CT]
              404  GCATGC              
SphI          272  GCATGC               GCATG^C
              404  GCATGC              


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

BbvI        BmyI        BpmI        BsgI        BsiYI       
BslI        Bsp1286I    BssKI       BstNI       Cac8I       
DdeI        EcoRII      Fnu4HI      ItaI        MnlI        
MvaI        MwoI        NlaIII      ScrFI       TseI        
TspRI