Restriction map for
pFN21AE5158
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaBI BsaHI BsaI BseAI BsgI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaI KasI KpnI MaeI
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NlaIII NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmeI PpuMI PshBI Psp1406I
PstI PvuII RcaI RsaI RsrII
SacI SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tsp45I Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 94 CGCG CG^CG
AcsI 379 AAATTC [GA]^AATT[CT]
AlwI 125 GGATC GGATC(4/5)
ApoI 379 AAATTC [GA]^AATT[CT]
BbrPI 40 CACGTG CAC^GTG
BbsI 18 GAAGAC GAAGAC(2/6)
BbvCI 397 GCTGAGG CCTCAGC(-5/-2)
BglI 59 GCCGCATAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 18 GAAGAC GAAGAC(2/6)
BsaAI 40 CACGTG [CT]AC^GT[GA]
BseRI 400 GAGGAG GAGGAG(10/8)
BsiYI 8 CCAGACTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 8 CCAGACTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 264 GAATGC GAATGC(1/-1)
BsrI 283 ACTGG ACTGG(1/-1)
BstF5I 5 CATCC GGATG(2/0)
BstUI 94 CGCG CG^CG
Cac8I 68 GCAAGC GC[ACGT]^[ACGT]GC
DraII 424 GGGGCCT [GA]G^G[ACGT]CC[CT]
EarI 392 GAAGAG CTCTTC(1/4)
EcoO109I 424 GGGGCCT [GA]G^G[ACGT]CC[CT]
FokI 5 CATCC GGATG(9/13)
HincII 217 GTCAAC GT[CT]^[GA]AC
HindII 217 GTCAAC GT[CT]^[GA]AC
Ksp632I 392 GAAGAG CTCTTC(1/4)
KspI 93 CCGCGG CCGC^GG
MaeII 41 ACGT A^CGT
MslI 300 CATTGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 93 CCGCGG C[AC]G^C[GT]G
MvnI 94 CGCG CG^CG
NotI 89 GCGGCCGC GC^GGCCGC
PmaCI 40 CACGTG CAC^GTG
PmlI 40 CACGTG CAC^GTG
PshAI 237 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 313 CGATCG CGAT^CG
SacII 93 CCGCGG CCGC^GG
SapI 392 GAAGAGC GCTCTTC(1/4)
SfaNI 114 GCATC GCATC(5/9)
TaqI 134 TCGA T^CGA
TspRI 246 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 134 TCGA T^CGA
XcmI 296 CCATCATTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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BcnI 23 CCCGG CC^[GC]GG
85 CCCGG
BsaWI 74 ACCGGA [AT]^CCGG[AT]
122 ACCGGA
BstNI 148 CCAGG CC^[AT]GG
435 CCTGG
Cfr13I 36 GGGCC G^G[ACGT]CC
425 GGGCC
DpnII 126 GATC ^GATC
314 GATC
EagI 57 CGGCCG C^GGCCG
90 CGGCCG
EclXI 57 CGGCCG C^GGCCG
90 CGGCCG
Eco52I 57 CGGCCG C^GGCCG
90 CGGCCG
EcoRII 148 CCAGG ^CC[AT]GG
435 CCTGG
HphI 261 GGTGA GGTGA(8/7)
117 TCACC
MboI 126 GATC ^GATC
314 GATC
MseI 187 TTAA T^TAA
410 TTAA
MvaI 148 CCAGG CC^[AT]GG
435 CCTGG
NciI 23 CCCGG CC^[GC]GG
85 CCCGG
NdeII 126 GATC ^GATC
314 GATC
Sau3AI 126 GATC ^GATC
314 GATC
Sau96I 36 GGGCC G^G[ACGT]CC
425 GGGCC
Tru9I 187 TTAA T^TAA
410 TTAA
Tsp509I 230 AATT ^AATT
380 AATT
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiEI
BssKI DdeI DsaI EaeI Fnu4HI
HaeIII HapII HpaII ItaI MboII
MnlI MspI MwoI NlaIV ScrFI
TseI