Restriction map for
pFN21AB7790
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI AscI Asp718I AspEI AspI
AvaII AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfaI BglI
BglII BlnI BlpI Bpu1102I BsaHI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I KasI KpnI KspI
MaeI MfeI MflI MluI MluNI
MroI MscI MspA1I MunI MvnI
MwoI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I Van91I
XbaI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AflII 646 CTTAAG C^TTAAG
AlwNI 4 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 181 GGGCCC GGGCC^C
AseI 934 ATTAAT AT^TAAT
AsnI 934 ATTAAT AT^TAAT
AvaI 1538 CTCGAG C^[CT]CG[GA]G
BfrI 646 CTTAAG C^TTAAG
BpmI 728 CTGGAG CTGGAG(16/14)
BsaAI 53 TACGTG [CT]AC^GT[GA]
BsaBI 21 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1415 ACCGGA [AT]^CCGG[AT]
BsgI 1302 CTGCAC GTGCAG(16/14)
BsmFI 839 GGGAC GGGAC(10/14)
BsoBI 1538 CTCGAG C^[CT]CG[GA]G
Bsp1407I 563 TGTACA T^GTACA
BsrDI 605 CATTGC GCAATG(2/0)
BsrGI 563 TGTACA T^GTACA
DpnII 1194 GATC ^GATC
DraI 1009 TTTAAA TTT^AAA
DraII 181 GGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 744 CCATGG C^C[GA][CT]GG
EcoNI 1493 CCTTCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 181 GGGCCCC [GA]G^G[ACGT]CC[CT]
HapII 1416 CCGG C^CGG
HincII 1393 GTTAAC GT[CT]^[GA]AC
HindII 1393 GTTAAC GT[CT]^[GA]AC
HindIII 924 AAGCTT A^AGCTT
HpaI 1393 GTTAAC GTT^AAC
HpaII 1416 CCGG C^CGG
MamI 21 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 1194 GATC ^GATC
MslI 1466 CATTCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1416 CCGG C^CGG
NcoI 744 CCATGG C^CATGG
NdeII 1194 GATC ^GATC
PaeR7I 1538 CTCGAG C^TCGAG
PshBI 934 ATTAAT AT^TAAT
PstI 424 CTGCAG CTGCA^G
Sau3AI 1194 GATC ^GATC
SfcI 424 CTGCAG C^T[GA][CT]AG
SspBI 563 TGTACA T^GTACA
XcmI 104 CCAACCCCGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1538 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AspHI 211 GAGCTC G[AT]GC[AT]^C
1419 GAGCTC
BbvI 3 GCAGC GCAGC(8/12)
93 GCAGC
BsaI 231 GAGACC GGTCTC(1/5)
989 GGTCTC
BsaJI 694 CCAAGG C^C[ACGT][ACGT]GG
744 CCATGG
BseRI 1422 CTCCTC GAGGAG(10/8)
889 GAGGAG
BsiHKAI 211 GAGCTC G[AT]GC[AT]^C
1419 GAGCTC
BssKI 288 CCTGG ^CC[ACGT]GG
797 CCTGG
BstEII 357 GGTGACC G^GT[ACGT]ACC
778 GGTCACC
BstNI 288 CCTGG CC^[AT]GG
797 CCTGG
BstPI 357 GGTGACC G^GT[ACGT]ACC
778 GGTCACC
Cac8I 457 GCTGGC GC[ACGT]^[ACGT]GC
1300 GCCTGC
Cfr13I 181 GGGCC G^G[ACGT]CC
182 GGCCC
EarI 526 GAAGAG CTCTTC(1/4)
717 GAAGAG
EcoO65I 357 GGTGACC G^GT[ACGT]ACC
778 GGTCACC
EcoRII 288 CCTGG ^CC[AT]GG
797 CCTGG
EcoT14I 694 CCAAGG C^C[AT][AT]GG
744 CCATGG
Fnu4HI 3 GCAGC GC^[ACGT]GC
93 GCAGC
ItaI 3 GCAGC GC^[ACGT]GC
93 GCAGC
Ksp632I 526 GAAGAG CTCTTC(1/4)
717 GAAGAG
MaeII 54 ACGT A^CGT
1254 ACGT
MvaI 288 CCTGG CC^[AT]GG
797 CCTGG
SacI 211 GAGCTC GAGCT^C
1419 GAGCTC
Sau96I 181 GGGCC G^G[ACGT]CC
182 GGCCC
ScrFI 288 CCTGG CC^[ACGT]GG
797 CCTGG
StuI 1298 AGGCCT AGG^CCT
1325 AGGCCT
StyI 694 CCAAGG C^C[AT][AT]GG
744 CCATGG
TseI 3 GCAGC G^C[AT]GC
93 GCAGC
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
BanII BbsI BmyI BpuAI BsiYI
BslI BsmAI Bsp1286I BsrI BstF5I
DdeI EcoRI FokI HaeIII HinfI
HphI MaeIII MboII MnlI MseI
NlaIII NlaIV PleI RsaI SfaNI
SmlI TaqI TfiI Tru9I Tsp45I
Tsp509I TspRI TthHB8I XmnI