Restriction map for
pFN21AE5474
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AvrII
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FauI FbaI FseI
HaeII Hin1I HincII HindII HindIII
HinfI HpaI HphI KasI KpnI
Ksp632I KspI MaeII MamI MfeI
MluI MroI MseI MspA1I MunI
MvnI NarI NciI NdeI NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI TfiI Tru9I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AciI 9 GCGG CCGC(-3/-1)
AviII 890 TGCGCA TGC^GCA
BanII 911 GAGCCC G[GA]GC[CT]^C
BglII 212 AGATCT A^GATCT
BmyI 911 GAGCCC G[AGT]GC[ACT]^C
BsaI 834 GGTCTC GGTCTC(1/5)
Bsp1286I 911 GAGCCC G[AGT]GC[ACT]^C
BsrFI 428 GCCGGC [GA]^CCGG[CT]
BstXI 824 CCAACAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 428 GCCGGC [GA]^CCGG[CT]
DraII 658 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 342 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 482 CCATGG C^C[GA][CT]GG
EcoO109I 658 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 303 ATGCAT ATGCA^T
FspI 890 TGCGCA TGC^GCA
HapII 429 CCGG C^CGG
HgaI 713 GACGC GACGC(5/10)
HpaII 429 CCGG C^CGG
MspI 429 CCGG C^CGG
NaeI 428 GCCGGC GCC^GGC
NcoI 482 CCATGG C^CATGG
NgoMIV 428 GCCGGC G^CCGGC
NheI 967 GCTAGC G^CTAGC
NsiI 303 ATGCAT ATGCA^T
SfcI 70 CTACAG C^T[GA][CT]AG
StuI 722 AGGCCT AGG^CCT
Tsp509I 147 AATT ^AATT
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BamHI 262 GGATCC G^GATCC
948 GGATCC
BpmI 208 CTGGAG CTGGAG(16/14)
464 CTGGAG
BsaJI 327 CCTTGG C^C[ACGT][ACGT]GG
482 CCATGG
BsiYI 327 CCTTGGCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
939 CCAGCACCAGG
BslI 327 CCTTGGCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
939 CCAGCACCAGG
BsmAI 556 GTCTC GTCTC(1/5)
835 GTCTC
BsrDI 563 CATTGC GCAATG(2/0)
863 CATTGC
BssKI 618 CCTGG ^CC[ACGT]GG
945 CCAGG
BstF5I 615 CATCC GGATG(2/0)
301 GGATG
BstNI 618 CCTGG CC^[AT]GG
945 CCAGG
CfoI 64 GCGC GCG^C
891 GCGC
EcoRII 618 CCTGG ^CC[AT]GG
945 CCAGG
EcoT14I 327 CCTTGG C^C[AT][AT]GG
482 CCATGG
FokI 615 CATCC GGATG(9/13)
301 GGATG
HhaI 64 GCGC GCG^C
891 GCGC
HinP1I 64 GCGC G^CGC
891 GCGC
MvaI 618 CCTGG CC^[AT]GG
945 CCAGG
ScrFI 618 CCTGG CC^[ACGT]GG
945 CCAGG
StyI 327 CCTTGG C^C[AT][AT]GG
482 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AfaI AluI AlwI BalI BbvI
BfaI BsgI BsrI BstYI Cac8I
Cfr13I DdeI DpnII EaeI Fnu4HI
HaeIII ItaI MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I SfaNI TseI
Tsp45I TspRI XhoII