Restriction map for
pFN21AE1730
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AvaII AviII AvrII BamHI BanII
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraI DraII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KspI MaeI MaeIII MamI
MfeI MluI MroI MseI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SfcI SfiI SfuI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI TfiI
Tru9I Tsp45I Tsp509I Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 492 GGTACC G^GTACC
Alw44I 630 GTGCAC G^TGCAC
AlwI 648 GATCC GGATC(4/5)
AlwNI 646 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 630 GTGCAC G^TGCAC
Asp718I 492 GGTACC G^GTACC
AspHI 630 GTGCAC G[AT]GC[AT]^C
BalI 194 TGGCCA TGG^CCA
BcnI 544 CCCGG CC^[GC]GG
BmyI 630 GTGCAC G[AGT]GC[ACT]^C
BsiHKAI 630 GTGCAC G[AT]GC[AT]^C
BsmFI 147 GTCCC GGGAC(10/14)
Bsp1286I 630 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 476 TGTACA T^GTACA
BsrFI 178 ACCGGC [GA]^CCGG[CT]
BsrGI 476 TGTACA T^GTACA
BsrI 464 ACTGG ACTGG(1/-1)
BstYI 647 AGATCC [GA]^GATC[CT]
Cfr10I 178 ACCGGC [GA]^CCGG[CT]
DpnII 648 GATC ^GATC
DraIII 549 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 282 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 489 GCGGG CCCGC(4/6)
HgaI 446 GCGTC GACGC(5/10)
HinfI 499 GACTC G^A[ACGT]TC
KpnI 492 GGTACC GGTAC^C
MaeII 392 ACGT A^CGT
MboI 648 GATC ^GATC
MflI 647 AGATCC [GA]^GATC[CT]
MluNI 194 TGGCCA TGG^CCA
MscI 194 TGGCCA TGG^CCA
MslI 669 CATGACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 435 CCGCTG C[AC]G^C[GT]G
NciI 544 CCCGG CC^[GC]GG
NdeII 648 GATC ^GATC
PflMI 404 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 499 GACTC GAGTC(4/5)
SapI 54 GCTCTTC GCTCTTC(1/4)
Sau3AI 648 GATC ^GATC
SexAI 550 ACCTGGT A^CC[AT]GGT
SgrAI 177 CACCGGCG C[GA]^CCGG[CT]G
SspBI 476 TGTACA T^GTACA
TaqI 470 TCGA T^CGA
TthHB8I 470 TCGA T^CGA
Van91I 404 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 197 CCATCGGCTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 647 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
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AluI 118 AGCT AG^CT
526 AGCT
BbeI 181 GGCGCC GGCGC^C
561 GGCGCC
BsgI 535 CTGCAC GTGCAG(16/14)
643 GTGCAG
BsmAI 574 GAGAC GTCTC(1/5)
448 GTCTC
BsmBI 447 CGTCTC CGTCTC(1/5)
574 GAGACG
BssHII 4 GCGCGC G^CGCGC
6 GCGCGC
BstF5I 303 CATCC GGATG(2/0)
420 CATCC
Cfr13I 396 GGGCC G^G[ACGT]CC
654 GGGCC
EaeI 101 TGGCCG [CT]^GGCC[GA]
194 TGGCCA
FokI 303 CATCC GGATG(9/13)
420 CATCC
HapII 179 CCGG C^CGG
545 CCGG
HpaII 179 CCGG C^CGG
545 CCGG
KasI 181 GGCGCC G^GCGCC
561 GGCGCC
MspI 179 CCGG C^CGG
545 CCGG
NarI 181 GGCGCC GG^CGCC
561 GGCGCC
Sau96I 396 GGGCC G^G[ACGT]CC
654 GGGCC
SfoI 181 GGCGCC GGC^GCC
561 GGCGCC
TspRI 459 CACTG CA[GC]TG[ACGT][ACGT]^
519 CAGTG
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AccII AciI AcyI AfaI BanI
BbvI BpmI BsaHI BsaJI BsiYI
BslI BspMI BssKI BstNI BstUI
Cac8I CfoI EarI EcoRII Fnu4HI
HaeII HaeIII HhaI Hin1I HinP1I
HphI ItaI Ksp632I MboII MnlI
MvaI MvnI MwoI NlaIII NlaIV
RsaI ScrFI SfaNI TseI