Restriction map for
pFN21AE5183
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# NO CUT ENZYMES ( 186 enzymes) #
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Acc65I AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiHKAI BsiWI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstUI BstZ17I Bsu36I CelII
Cfr13I ClaI CpoI DdeI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FokI
FseI FspI HgaI HincII HindII
HpaI HphI KasI KpnI KspI
MaeIII MamI MfeI MluI MluNI
MroI MscI MseI MslI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp45I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 157 GACGTC GACGT^C
AciI 100 GCGG CCGC(-3/-1)
AcyI 157 GACGTC G[GA]^CG[CT]C
AfaI 193 GTAC GT^AC
AvrII 33 CCTAGG C^CTAGG
BbsI 154 GAAGAC GAAGAC(2/6)
BfaI 34 CTAG C^TAG
BglII 74 AGATCT A^GATCT
BlnI 33 CCTAGG C^CTAGG
BpuAI 154 GAAGAC GAAGAC(2/6)
BsaHI 157 GACGTC G[GA]^CG[CT]C
BsiEI 108 CGACCG CG[GA][CT]^CG
BsmAI 217 GTCTC GTCTC(1/5)
BsmBI 216 CGTCTC CGTCTC(1/5)
BsrDI 162 CATTGC GCAATG(2/0)
BsrFI 110 ACCGGC [GA]^CCGG[CT]
BsrI 188 CCAGT ACTGG(1/-1)
BstAPI 238 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 168 CCAGGGTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 74 AGATCT [GA]^GATC[CT]
Cfr10I 110 ACCGGC [GA]^CCGG[CT]
DpnII 75 GATC ^GATC
EarI 307 GAAGAG CTCTTC(1/4)
HaeII 55 AGCGCC [GA]GCGC^[CT]
HaeIII 334 GGCC GG^CC
HapII 111 CCGG C^CGG
Hin1I 157 GACGTC G[GA]^CG[CT]C
HindIII 181 AAGCTT A^AGCTT
HpaII 111 CCGG C^CGG
Ksp632I 307 GAAGAG CTCTTC(1/4)
MaeI 34 CTAG C^TAG
MboI 75 GATC ^GATC
MflI 74 AGATCT [GA]^GATC[CT]
MspI 111 CCGG C^CGG
NdeII 75 GATC ^GATC
NlaIII 324 CATG CATG^
RsaI 193 GTAC GT^AC
Sau3AI 75 GATC ^GATC
TaqI 95 TCGA T^CGA
TthHB8I 95 TCGA T^CGA
XhoII 74 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AluI 134 AGCT AG^CT
182 AGCT
AlwNI 230 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
239 CAGCCCCTG
BsiYI 196 CCTGCTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
244 CCTGCTGCTGG
BslI 196 CCTGCTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
244 CCTGCTGCTGG
BspMI 20 ACCTGC ACCTGC(4/8)
195 ACCTGC
BssKI 168 CCAGG ^CC[ACGT]GG
229 CCAGG
BstNI 168 CCAGG CC^[AT]GG
229 CCAGG
CfoI 3 GCGC GCG^C
56 GCGC
EcoRII 168 CCAGG ^CC[AT]GG
229 CCAGG
EcoT14I 33 CCTAGG C^C[AT][AT]GG
59 CCAAGG
HhaI 3 GCGC GCG^C
56 GCGC
HinP1I 3 GCGC G^CGC
56 GCGC
HinfI 301 GAGTC G^A[ACGT]TC
310 GAGTC
MaeII 8 ACGT A^CGT
158 ACGT
MvaI 168 CCAGG CC^[AT]GG
229 CCAGG
PleI 301 GAGTC GAGTC(4/5)
310 GAGTC
PstI 236 CTGCAG CTGCA^G
266 CTGCAG
ScrFI 168 CCAGG CC^[ACGT]GG
229 CCAGG
StyI 33 CCTAGG C^C[AT][AT]GG
59 CCAAGG
TspRI 129 CAGTG CA[GC]TG[ACGT][ACGT]^
189 CAGTG
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
BbvI BsaJI BseRI Cac8I Fnu4HI
ItaI MboII MnlI MwoI SfcI
TseI