Restriction map for
pFN21AE5659
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI AocI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvrII BalI BanI
BbeI BbrPI BcgI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BseRI BsiEI
BsiWI BsmBI BsmI BspDI BspLU11I
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT22I FbaI FseI HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SwaI Tth111I Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 398 TCCGGA T^CCGGA
AcsI 1204 GAATTT [GA]^AATT[CT]
Alw44I 76 GTGCAC G^TGCAC
AlwNI 81 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 76 GTGCAC G^TGCAC
ApoI 1204 GAATTT [GA]^AATT[CT]
Asp700I 129 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 321 TGCGCA TGC^GCA
BamHI 437 GGATCC G^GATCC
BbsI 477 GAAGAC GAAGAC(2/6)
BbvCI 1410 CCTCAGC CCTCAGC(-5/-2)
BpuAI 477 GAAGAC GAAGAC(2/6)
BsaAI 211 TACGTG [CT]AC^GT[GA]
BsaI 904 GAGACC GGTCTC(1/5)
BsaWI 398 TCCGGA [AT]^CCGG[AT]
BseAI 398 TCCGGA T^CCGGA
BsgI 836 GTGCAG GTGCAG(16/14)
Bsp1407I 848 TGTACA T^GTACA
BspEI 398 TCCGGA T^CCGGA
BspHI 944 TCATGA T^CATGA
BsrBI 1473 CCGCTC CCGCTC(-3/-3)
BsrDI 425 GCAATG GCAATG(2/0)
BsrFI 195 GCCGGT [GA]^CCGG[CT]
BsrGI 848 TGTACA T^GTACA
BsrI 138 CCAGT ACTGG(1/-1)
BssHII 521 GCGCGC G^CGCGC
BstAPI 1032 GCAAGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 437 GGATCC [GA]^GATC[CT]
Cfr10I 195 GCCGGT [GA]^CCGG[CT]
DsaI 20 CCGTGG C^C[GA][CT]GG
EaeI 878 TGGCCG [CT]^GGCC[GA]
EcoRV 1402 GATATC GAT^ATC
FspI 321 TGCGCA TGC^GCA
HincII 1390 GTTGAC GT[CT]^[GA]AC
HindII 1390 GTTGAC GT[CT]^[GA]AC
MflI 437 GGATCC [GA]^GATC[CT]
MroI 398 TCCGGA T^CCGGA
PpuMI 788 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 84 CAGCTG CAG^CTG
RcaI 944 TCATGA T^CATGA
SmaI 785 CCCGGG CCC^GGG
SspBI 848 TGTACA T^GTACA
SspI 154 AATATT AAT^ATT
StuI 1320 AGGCCT AGG^CCT
XhoII 437 GGATCC [GA]^GATC[CT]
XmaCI 785 CCCGGG C^CCGGG
XmaI 785 CCCGGG C^CCGGG
XmnI 129 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 312 CGCG CG^CG
522 CGCG
AspHI 76 GTGCAC G[AT]GC[AT]^C
754 GAGCAC
BanII 216 GGGCTC G[GA]GC[CT]^C
931 GGGCTC
BciVI 1138 GGATAC GTATCC(6/5)
1439 GTATCC
BpmI 643 CTCCAG CTGGAG(16/14)
1313 CTGGAG
BsiHKAI 76 GTGCAC G[AT]GC[AT]^C
754 GAGCAC
BsmAI 904 GAGAC GTCTC(1/5)
1121 GAGAC
BssSI 808 CTCGTG CACGAG(-5/-1)
1087 CTCGTG
BstUI 312 CGCG CG^CG
522 CGCG
DraII 102 AGGCCCT [GA]G^G[ACGT]CC[CT]
788 GGGACCT
EcoO109I 102 AGGCCCT [GA]G^G[ACGT]CC[CT]
788 GGGACCT
EcoT14I 483 CCAAGG C^C[AT][AT]GG
1151 CCAAGG
MslI 228 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1083 CACCCTCGTG
MspA1I 84 CAGCTG C[AC]G^C[GT]G
1072 CAGCGG
MvnI 312 CGCG CG^CG
522 CGCG
PleI 1180 GACTC GAGTC(4/5)
14 GAGTC
StyI 483 CCAAGG C^C[AT][AT]GG
1151 CCAAGG
TspRI 834 CAGTG CA[GC]TG[ACGT][ACGT]^
1269 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AlwI Aor51HI
AvaI AvaII BbvI BcnI BmyI
BsaJI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EarI Eco47III EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TthHB8I