Restriction map for
pFN21AE0956
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI Alw44I AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BamHI BbvCI BcgI BciVI BclI
BfaI BfrI BlnI BlpI Bpu1102I
BsaBI BsaI BsiWI BsmAI BsmBI
BsmI BspDI BspHI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI MaeI
MaeIII MamI MfeI MluI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
SspI StuI SwaI TfiI Tsp45I
Tsp509I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccIII 774 TCCGGA T^CCGGA
AflIII 494 ACATGT A^C[GA][CT]GT
AhdI 829 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 131 GGGCCC GGGCC^C
AspEI 829 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 397 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 10 GGCGCC GGCGC^C
BbrPI 957 CACGTG CAC^GTG
BglI 74 GCCCCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 779 AGATCT A^GATCT
BsaAI 957 CACGTG [CT]AC^GT[GA]
BsaWI 774 TCCGGA [AT]^CCGG[AT]
BseAI 774 TCCGGA T^CCGGA
Bsp1407I 584 TGTACA T^GTACA
BspEI 774 TCCGGA T^CCGGA
BspLU11I 494 ACATGT A^CATGT
BsrDI 708 CATTGC GCAATG(2/0)
BsrGI 584 TGTACA T^GTACA
BstXI 965 CCAAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eam1105I 829 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 965 CCAAGG C^C[AT][AT]GG
HincII 796 GTCAAC GT[CT]^[GA]AC
HindII 796 GTCAAC GT[CT]^[GA]AC
KasI 10 GGCGCC G^GCGCC
KspI 35 CCGCGG CCGC^GG
MroI 774 TCCGGA T^CCGGA
MseI 1307 TTAA T^TAA
NarI 10 GGCGCC GG^CGCC
PflFI 397 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 957 CACGTG CAC^GTG
PmlI 957 CACGTG CAC^GTG
PvuII 922 CAGCTG CAG^CTG
SacII 35 CCGCGG CCGC^GG
SbfI 432 CCTGCAGG CCTGCA^GG
SfoI 10 GGCGCC GGC^GCC
Sse8387I 432 CCTGCAGG CCTGCA^GG
SspBI 584 TGTACA T^GTACA
StyI 965 CCAAGG C^C[AT][AT]GG
Tru9I 1307 TTAA T^TAA
Tth111I 397 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 10 GGCGCC G[GA]^CG[CT]C
39 GGCGTC
AspHI 445 GAGCAC G[AT]GC[AT]^C
1012 GAGCAC
BalI 120 TGGCCA TGG^CCA
1205 TGGCCA
BanI 10 GGCGCC G^G[CT][GA]CC
754 GGCACC
BbsI 412 GTCTTC GAAGAC(2/6)
451 GTCTTC
BpuAI 412 GTCTTC GAAGAC(2/6)
451 GTCTTC
BsaHI 10 GGCGCC G[GA]^CG[CT]C
39 GGCGTC
BsiEI 63 CGGCCG CG[GA][CT]^CG
1445 CGGCCG
BsiHKAI 445 GAGCAC G[AT]GC[AT]^C
1012 GAGCAC
BssHII 68 GCGCGC G^CGCGC
70 GCGCGC
BstAPI 314 GCAAGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
549 GCAGAACGTGC
DdeI 1081 CTGAG C^T[ACGT]AG
1364 CTGAG
DsaI 35 CCGCGG C^C[GA][CT]GG
905 CCGTGG
EagI 63 CGGCCG C^GGCCG
1445 CGGCCG
EclXI 63 CGGCCG C^GGCCG
1445 CGGCCG
Eco52I 63 CGGCCG C^GGCCG
1445 CGGCCG
Hin1I 10 GGCGCC G[GA]^CG[CT]C
39 GGCGTC
MluNI 120 TGGCCA TGG^CCA
1205 TGGCCA
MscI 120 TGGCCA TGG^CCA
1205 TGGCCA
NaeI 79 GCCGGC GCC^GGC
1060 GCCGGC
NgoMIV 79 GCCGGC G^CCGGC
1060 GCCGGC
NspI 494 ACATGT [GA]CATG^[CT]
684 ACATGC
PpuMI 527 GGGACCT [GA]G^G[AT]CC[CT]
1334 AGGACCT
PstI 433 CTGCAG CTGCA^G
544 CTGCAG
SfcI 433 CTGCAG C^T[GA][CT]AG
544 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DpnII DraII
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmaI
TaqI TseI TspRI TthHB8I XcmI
XhoII XmaCI XmaI