Restriction map for
pFN21AB9968
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BbsI BciVI BclI
BfrI BglII BlnI BpuAI BsaAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspLU11I BsrGI BssHII BstBI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoT22I FbaI FseI
FspI HindIII KpnI KspI MfeI
MluI MunI NcoI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SacII SapI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
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AcyI 124 GGCGCC G[GA]^CG[CT]C
AlwNI 133 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 603 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 124 GGCGCC GGCGC^C
BbvCI 1926 CCTCAGC CCTCAGC(-5/-2)
BcgI 69 CGAGACCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 521 CTAG C^TAG
BlpI 303 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 303 GCTCAGC GC^T[ACGT]AGC
BsaBI 369 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 124 GGCGCC G[GA]^CG[CT]C
BspDI 1042 ATCGAT AT^CGAT
BspHI 1313 TCATGA T^CATGA
BsrBI 43 CCGCTC CCGCTC(-3/-3)
BsrDI 422 CATTGC GCAATG(2/0)
BsrFI 1344 GCCGGC [GA]^CCGG[CT]
BssSI 178 CTCGTG CACGAG(-5/-1)
Bst1107I 1694 GTATAC GTA^TAC
BstAPI 1233 GCAGACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 311 GGTCACC G^GT[ACGT]ACC
BstPI 311 GGTCACC G^GT[ACGT]ACC
BstZ17I 1694 GTATAC GTA^TAC
CelII 303 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1344 GCCGGC [GA]^CCGG[CT]
ClaI 1042 ATCGAT AT^CGAT
DrdI 1702 GACTTTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1986 GAAGAG CTCTTC(1/4)
EcoNI 357 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 311 GGTCACC G^GT[ACGT]ACC
EcoRV 491 GATATC GAT^ATC
EcoT14I 750 CCAAGG C^C[AT][AT]GG
HgaI 963 GACGC GACGC(5/10)
Hin1I 124 GGCGCC G[GA]^CG[CT]C
KasI 124 GGCGCC G^GCGCC
Ksp632I 1986 GAAGAG CTCTTC(1/4)
MaeI 521 CTAG C^TAG
MamI 369 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1344 GCCGGC GCC^GGC
NarI 124 GGCGCC GG^CGCC
NdeI 2011 CATATG CA^TATG
NgoMIV 1344 GCCGGC G^CCGGC
PaeR7I 1174 CTCGAG C^TCGAG
PpuMI 624 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 1313 TCATGA T^CATGA
SalI 808 GTCGAC G^TCGAC
SfoI 124 GGCGCC GGC^GCC
SmaI 120 CCCGGG CCC^GGG
StyI 750 CCAAGG C^C[AT][AT]GG
XcmI 260 CCAACGGAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1174 CTCGAG C^TCGAG
XmaCI 120 CCCGGG C^CCGGG
XmaI 120 CCCGGG C^CCGGG
XmnI 603 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 808 GTCGAC GT^[AC][GT]AC
1694 GTATAC
AccII 1008 CGCG CG^CG
1068 CGCG
AflIII 1151 ACACGT A^C[GA][CT]GT
1208 ACGTGT
Alw44I 976 GTGCAC G^TGCAC
1882 GTGCAC
AocI 104 CCTCAGG CC^T[ACGT]AGG
317 CCTCAGG
ApaI 322 GGGCCC GGGCC^C
985 GGGCCC
ApaLI 976 GTGCAC G^TGCAC
1882 GTGCAC
AvaI 120 CCCGGG C^[CT]CG[GA]G
1174 CTCGAG
BalI 1055 TGGCCA TGG^CCA
1618 TGGCCA
BglI 1019 GCCGGACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1048 GCCATGATGGC
BsoBI 120 CCCGGG C^[CT]CG[GA]G
1174 CTCGAG
BstUI 1008 CGCG CG^CG
1068 CGCG
BstXI 341 CCATCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
537 CCAACTGGATGG
BstYI 368 AGATCC [GA]^GATC[CT]
379 AGATCC
Bsu36I 104 CCTCAGG CC^T[ACGT]AGG
317 CCTCAGG
DsaI 347 CCGTGG C^C[GA][CT]GG
1776 CCACGG
EaeI 1055 TGGCCA [CT]^GGCC[GA]
1618 TGGCCA
Eco81I 104 CCTCAGG CC^T[ACGT]AGG
317 CCTCAGG
EcoRI 548 GAATTC G^AATTC
2038 GAATTC
HaeII 124 GGCGCC [GA]GCGC^[CT]
1118 AGCGCC
HpaI 643 GTTAAC GTT^AAC
696 GTTAAC
MaeII 1153 ACGT A^CGT
1208 ACGT
MflI 368 AGATCC [GA]^GATC[CT]
379 AGATCC
MluNI 1055 TGGCCA TGG^CCA
1618 TGGCCA
MscI 1055 TGGCCA TGG^CCA
1618 TGGCCA
MseI 644 TTAA T^TAA
697 TTAA
MvnI 1008 CGCG CG^CG
1068 CGCG
PshAI 684 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1299 GACAAATGTC
PstI 1231 CTGCAG CTGCA^G
1887 CTGCAG
PvuII 883 CAGCTG CAG^CTG
897 CAGCTG
ScaI 737 AGTACT AGT^ACT
1178 AGTACT
Tru9I 644 TTAA T^TAA
697 TTAA
Tsp45I 312 GTCAC ^GT[GC]AC
1711 GTCAC
XhoII 368 AGATCC [GA]^GATC[CT]
379 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccIII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspEI BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MroI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TfiI TseI Tsp509I TspRI
TthHB8I