Restriction map for
pFN21AE0089
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccII AclI AcsI
AcyI AflII AgeI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AvaI AviII AvrII
BanI BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaHI BsaI BsgI
BsiWI BsmI BsoBI BspDI BspHI
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
Eco47III EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MfeI MluI MspA1I
MunI MvnI NaeI NarI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I StuI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 1064 TCCGGA T^CCGGA
AhdI 336 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1885 GTGCAC G^TGCAC
AocI 365 CCTCAGG CC^T[ACGT]AGG
ApaLI 1885 GTGCAC G^TGCAC
AspEI 336 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 159 GGATCC G^GATCC
BanII 599 GAGCTC G[GA]GC[CT]^C
BbsI 1513 GAAGAC GAAGAC(2/6)
BclI 516 TGATCA T^GATCA
BcnI 1644 CCGGG CC^[GC]GG
BpmI 1550 CTGGAG CTGGAG(16/14)
BpuAI 1513 GAAGAC GAAGAC(2/6)
BsaAI 39 TACGTA [CT]AC^GT[GA]
BsaBI 154 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1064 TCCGGA T^CCGGA
BsiEI 2071 CGGCCG CG[GA][CT]^CG
BsiYI 326 CCCAACGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 326 CCCAACGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 1214 GAGACG CGTCTC(1/5)
BsmFI 665 GGGAC GGGAC(10/14)
BspEI 1064 TCCGGA T^CCGGA
BspLU11I 272 ACATGT A^CATGT
BstAPI 972 GCAGGATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 365 CCTCAGG CC^T[ACGT]AGG
DsaI 1772 CCATGG C^C[GA][CT]GG
EagI 2071 CGGCCG C^GGCCG
Eam1105I 336 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2071 CGGCCG C^GGCCG
Eco52I 2071 CGGCCG C^GGCCG
Eco81I 365 CCTCAGG CC^T[ACGT]AGG
FauI 663 GCGGG CCCGC(4/6)
FbaI 516 TGATCA T^GATCA
HgaI 1569 GACGC GACGC(5/10)
HindIII 1557 AAGCTT A^AGCTT
MamI 154 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1064 TCCGGA T^CCGGA
MslI 2028 CACTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 1644 CCGGG CC^[GC]GG
NcoI 1772 CCATGG C^CATGG
NdeI 476 CATATG CA^TATG
NheI 981 GCTAGC G^CTAGC
NspI 272 ACATGT [GA]CATG^[CT]
PstI 1316 CTGCAG CTGCA^G
SacI 599 GAGCTC GAGCT^C
SalI 1289 GTCGAC G^TCGAC
SapI 792 GCTCTTC GCTCTTC(1/4)
SfaNI 1361 GCATC GCATC(5/9)
SnaBI 39 TACGTA TAC^GTA
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccI 739 GTAGAC GT^[AC][GT]AC
1289 GTCGAC
AflIII 272 ACATGT A^C[GA][CT]GT
1276 ACACGT
AlwNI 368 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
557 CAGAGACTG
AspHI 599 GAGCTC G[AT]GC[AT]^C
1885 GTGCAC
AvaII 335 GGACC G^G[AT]CC
1259 GGTCC
BalI 52 TGGCCA TGG^CCA
292 TGGCCA
BsaWI 1064 TCCGGA [AT]^CCGG[AT]
1310 ACCGGA
BseRI 356 CTCCTC GAGGAG(10/8)
863 GAGGAG
BsiHKAI 599 GAGCTC G[AT]GC[AT]^C
1885 GTGCAC
DraI 124 TTTAAA TTT^AAA
745 TTTAAA
HincII 1289 GTCGAC GT[CT]^[GA]AC
1858 GTTGAC
HindII 1289 GTCGAC GT[CT]^[GA]AC
1858 GTTGAC
MluNI 52 TGGCCA TGG^CCA
292 TGGCCA
MscI 52 TGGCCA TGG^CCA
292 TGGCCA
SfcI 347 CTACAG C^T[GA][CT]AG
1316 CTGCAG
SmlI 378 CTCAAG C^T[CT][GA]AG
1491 CTTGAG
TaqI 603 TCGA T^CGA
1290 TCGA
TthHB8I 603 TCGA T^CGA
1290 TCGA
XcmI 87 CCAAAGACCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1246 CCAATTATTAACTGG
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BfaI BmyI BsaJI BsmAI Bsp1286I
Bsp1407I BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SspBI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII