Restriction map for
pFN21AE9515
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI AlwI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HinfI
HpaI KpnI Ksp632I MaeI MamI
MfeI MflI MluI MroI MseI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SalI SapI SbfI
ScaI SfiI SfuI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AcyI 815 GGCGCC G[GA]^CG[CT]C
AlwNI 151 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 21 GGGCCC GGGCC^C
AvaI 17 CTCGGG C^[CT]CG[GA]G
AviII 1037 TGCGCA TGC^GCA
BbeI 815 GGCGCC GGCGC^C
BbrPI 598 CACGTG CAC^GTG
BcgI 88 GCAGAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 544 GCCATCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 131 CTCCAG CTGGAG(16/14)
BsaAI 598 CACGTG [CT]AC^GT[GA]
BsaHI 815 GGCGCC G[GA]^CG[CT]C
BsoBI 17 CTCGGG C^[CT]CG[GA]G
BsrBI 343 CCGCTC CCGCTC(-3/-3)
BstF5I 720 CATCC GGATG(2/0)
DrdI 472 GACCGCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 627 CCTCTGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1077 CCATGG C^C[AT][AT]GG
FokI 720 CATCC GGATG(9/13)
FspI 1037 TGCGCA TGC^GCA
Hin1I 815 GGCGCC G[GA]^CG[CT]C
KasI 815 GGCGCC G^GCGCC
KspI 560 CCGCGG CCGC^GG
MslI 462 CATCGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 815 GGCGCC GG^CGCC
NcoI 1077 CCATGG C^CATGG
PmaCI 598 CACGTG CAC^GTG
PmlI 598 CACGTG CAC^GTG
PpuMI 902 GGGACCT [GA]G^G[AT]CC[CT]
SacI 604 GAGCTC GAGCT^C
SacII 560 CCGCGG CCGC^GG
SexAI 404 ACCTGGT A^CC[AT]GGT
SfoI 815 GGCGCC GGC^GCC
SmlI 607 CTCAAG C^T[CT][GA]AG
StuI 92 AGGCCT AGG^CCT
StyI 1077 CCATGG C^C[AT][AT]GG
XcmI 458 CCACCATCGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
BalI 542 TGGCCA TGG^CCA
1080 TGGCCA
BanI 768 GGTGCC G^G[CT][GA]CC
815 GGCGCC
BbsI 409 GTCTTC GAAGAC(2/6)
886 GTCTTC
BbvCI 522 CCTCAGC CCTCAGC(-5/-2)
498 GCTGAGG
BcnI 738 CCGGG CC^[GC]GG
772 CCGGG
BpuAI 409 GTCTTC GAAGAC(2/6)
886 GTCTTC
BseRI 634 GAGGAG GAGGAG(10/8)
1030 GAGGAG
BsmFI 803 GGGAC GGGAC(10/14)
902 GGGAC
BspMI 701 ACCTGC ACCTGC(4/8)
893 ACCTGC
BsrI 800 ACTGG ACTGG(1/-1)
962 ACTGG
BstEII 426 GGTCACC G^GT[ACGT]ACC
693 GGTCACC
BstPI 426 GGTCACC G^GT[ACGT]ACC
693 GGTCACC
BstXI 370 CCACGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
461 CCATCGCAGTGG
DdeI 499 CTGAG C^T[ACGT]AG
523 CTCAG
DpnII 186 GATC ^GATC
252 GATC
DraIII 274 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
616 CACGACGTG
DsaI 560 CCGCGG C^C[GA][CT]GG
1077 CCATGG
EaeI 542 TGGCCA [CT]^GGCC[GA]
1080 TGGCCA
EcoO65I 426 GGTCACC G^GT[ACGT]ACC
693 GGTCACC
HgaI 127 GACGC GACGC(5/10)
203 GCGTC
MboI 186 GATC ^GATC
252 GATC
MluNI 542 TGGCCA TGG^CCA
1080 TGGCCA
MscI 542 TGGCCA TGG^CCA
1080 TGGCCA
NciI 738 CCGGG CC^[GC]GG
772 CCGGG
NdeII 186 GATC ^GATC
252 GATC
PstI 157 CTGCAG CTGCA^G
418 CTGCAG
Sau3AI 186 GATC ^GATC
252 GATC
SfaNI 168 GCATC GCATC(5/9)
509 GCATC
SgrAI 56 CGCCGGCG C[GA]^CCGG[CT]G
817 CGCCGGCG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AluI Alw44I ApaLI
AspHI AvaII BanII BbvI BmyI
BsaJI BsgI BsiHKAI BsiYI BslI
Bsp1286I BsrFI BssKI BstAPI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DraII EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeII MaeIII
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NaeI NgoMIV NlaIII
NlaIV PvuII Sau96I ScrFI SfcI
TaqI TseI Tsp45I TspRI TthHB8I