Restriction map for
pFN21AE5229
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BbrPI BbsI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
EaeI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
MaeI MamI MfeI MluI MluNI
MroI MscI MslI MunI NaeI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp45I TspRI
Tth111I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AfaI 369 GTAC GT^AC
Aor51HI 359 AGCGCT AGC^GCT
AspHI 394 GAGCTC G[AT]GC[AT]^C
AvaI 56 CCCGGG C^[CT]CG[GA]G
BamHI 227 GGATCC G^GATCC
BanI 145 GGCGCC G^G[CT][GA]CC
BanII 394 GAGCTC G[GA]GC[CT]^C
BbeI 145 GGCGCC GGCGC^C
BbvCI 169 GCTGAGG CCTCAGC(-5/-2)
BcgI 330 GCAGCTCTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 394 GAGCTC G[AGT]GC[ACT]^C
BpmI 343 CTGGAG CTGGAG(16/14)
BsaAI 370 TACGTG [CT]AC^GT[GA]
BsiHKAI 394 GAGCTC G[AT]GC[AT]^C
BsmFI 527 GGGAC GGGAC(10/14)
BsoBI 56 CCCGGG C^[CT]CG[GA]G
Bsp1286I 394 GAGCTC G[AGT]GC[ACT]^C
BsrFI 293 ACCGGC [GA]^CCGG[CT]
BsrI 366 CCAGT ACTGG(1/-1)
BstXI 275 CCAAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 227 GGATCC [GA]^GATC[CT]
Cfr10I 293 ACCGGC [GA]^CCGG[CT]
DraII 278 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 306 GACGCGCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 323 CCGCGG C^C[GA][CT]GG
Eco47III 359 AGCGCT AGC^GCT
EcoNI 270 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 278 AGGGCCT [GA]G^G[ACGT]CC[CT]
FauI 464 GCGGG CCCGC(4/6)
HphI 320 TCACC GGTGA(8/7)
KasI 145 GGCGCC G^GCGCC
KspI 323 CCGCGG CCGC^GG
MaeIII 179 GTAAC ^GT[ACGT]AC
MflI 227 GGATCC [GA]^GATC[CT]
MseI 410 TTAA T^TAA
NarI 145 GGCGCC GG^CGCC
PflMI 366 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 369 GTAC GT^AC
SacI 394 GAGCTC GAGCT^C
SacII 323 CCGCGG CCGC^GG
SfoI 145 GGCGCC GGC^GCC
SmaI 56 CCCGGG CCC^GGG
SmlI 130 CTCAAG C^T[CT][GA]AG
TfiI 455 GATTC G^A[AT]TC
Tru9I 410 TTAA T^TAA
Tsp509I 196 AATT ^AATT
Van91I 366 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 227 GGATCC [GA]^GATC[CT]
XmaCI 56 CCCGGG C^CCGGG
XmaI 56 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 43 GACGCC G[GA]^CG[CT]C
145 GGCGCC
AlwI 228 GATCC GGATC(4/5)
227 GGATC
BsaHI 43 GACGCC G[GA]^CG[CT]C
145 GGCGCC
BsaI 421 GAGACC GGTCTC(1/5)
60 GGTCTC
BstF5I 255 CATCC GGATG(2/0)
267 CATCC
Cac8I 396 GCTCGC GC[ACGT]^[ACGT]GC
501 GCCAGC
DpnII 228 GATC ^GATC
264 GATC
EcoT14I 245 CCAAGG C^C[AT][AT]GG
275 CCAAGG
FokI 255 CATCC GGATG(9/13)
267 CATCC
Hin1I 43 GACGCC G[GA]^CG[CT]C
145 GGCGCC
MboI 228 GATC ^GATC
264 GATC
MspA1I 203 CAGCGG C[AC]G^C[GT]G
323 CCGCGG
NdeII 228 GATC ^GATC
264 GATC
Sau3AI 228 GATC ^GATC
264 GATC
SfaNI 32 GATGC GCATC(5/9)
241 GATGC
StyI 245 CCAAGG C^C[AT][AT]GG
275 CCAAGG
TaqI 30 TCGA T^CGA
64 TCGA
TthHB8I 30 TCGA T^CGA
64 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BcnI BsaJI BsiYI BslI BsmAI
BssKI BstNI BstUI CfoI Cfr13I
DdeI EcoRII Fnu4HI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII ItaI MaeII MboII MnlI
MspI MvaI MvnI MwoI NciI
NlaIV PleI Sau96I ScrFI TseI