Restriction map for
pFN21AE6620
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvrII BbeI BbrPI
BciVI BclI BfrI BglI BglII
BlnI BsaAI BsaBI BsaI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BspDI BspHI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeII MamI MfeI
MluI MseI MunI NaeI NarI
NdeI NgoMIV NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI Tru9I Tsp509I
Tth111I Van91I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 328 TCCGGA T^CCGGA
AcyI 712 GGCGTC G[GA]^CG[CT]C
AfaI 501 GTAC GT^AC
AocI 77 CCTCAGG CC^T[ACGT]AGG
AvaI 268 CTCGAG C^[CT]CG[GA]G
AviII 950 TGCGCA TGC^GCA
BamHI 851 GGATCC G^GATCC
BanI 193 GGCACC G^G[CT][GA]CC
BbsI 21 GTCTTC GAAGAC(2/6)
BbvCI 934 GCTGAGG CCTCAGC(-5/-2)
BcgI 763 CGACTGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 839 CCCGG CC^[GC]GG
BfaI 319 CTAG C^TAG
BpuAI 21 GTCTTC GAAGAC(2/6)
BsaHI 712 GGCGTC G[GA]^CG[CT]C
BsaWI 328 TCCGGA [AT]^CCGG[AT]
BseAI 328 TCCGGA T^CCGGA
BsoBI 268 CTCGAG C^[CT]CG[GA]G
Bsp1407I 500 TGTACA T^GTACA
BspEI 328 TCCGGA T^CCGGA
BspMI 530 ACCTGC ACCTGC(4/8)
BsrGI 500 TGTACA T^GTACA
BstYI 851 GGATCC [GA]^GATC[CT]
Bsu36I 77 CCTCAGG CC^T[ACGT]AGG
DsaI 398 CCATGG C^C[GA][CT]GG
Eco81I 77 CCTCAGG CC^T[ACGT]AGG
FspI 950 TGCGCA TGC^GCA
Hin1I 712 GGCGTC G[GA]^CG[CT]C
MaeI 319 CTAG C^TAG
MflI 851 GGATCC [GA]^GATC[CT]
MroI 328 TCCGGA T^CCGGA
MslI 399 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 839 CCCGG CC^[GC]GG
NcoI 398 CCATGG C^CATGG
NheI 318 GCTAGC G^CTAGC
PaeR7I 268 CTCGAG C^TCGAG
PleI 337 GAGTC GAGTC(4/5)
PpuMI 6 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 754 CAGCTG CAG^CTG
RsaI 501 GTAC GT^AC
SspBI 500 TGTACA T^GTACA
SspI 571 AATATT AAT^ATT
TaqI 269 TCGA T^CGA
TfiI 177 GAATC G^A[AT]TC
TthHB8I 269 TCGA T^CGA
XhoI 268 CTCGAG C^TCGAG
XhoII 851 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 393 CGCG CG^CG
907 CGCG
AlwNI 796 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
919 CAGGAGCTG
BalI 683 TGGCCA TGG^CCA
725 TGGCCA
BlpI 39 GCTAAGC GC^T[ACGT]AGC
924 GCTGAGC
BpmI 169 CTGGAG CTGGAG(16/14)
757 CTGGAG
Bpu1102I 39 GCTAAGC GC^T[ACGT]AGC
924 GCTGAGC
BsgI 181 CTGCAC GTGCAG(16/14)
771 CTGCAC
BsmFI 8 GTCCC GGGAC(10/14)
508 GTCCC
BsrDI 102 CATTGC GCAATG(2/0)
780 GCAATG
BstEII 405 GGTGACC G^GT[ACGT]ACC
834 GGTGACC
BstF5I 331 GGATG GGATG(2/0)
540 GGATG
BstPI 405 GGTGACC G^GT[ACGT]ACC
834 GGTGACC
BstUI 393 CGCG CG^CG
907 CGCG
BstXI 56 CCAGGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
728 CCAATGTCCTGG
CelII 39 GCTAAGC GC^T[ACGT]AGC
924 GCTGAGC
DraII 6 GGGTCCC [GA]G^G[ACGT]CC[CT]
897 AGGCCCC
EcoO109I 6 GGGTCCC [GA]G^G[ACGT]CC[CT]
897 AGGCCCC
EcoO65I 405 GGTGACC G^GT[ACGT]ACC
834 GGTGACC
FauI 931 CCCGC CCCGC(4/6)
106 GCGGG
FokI 331 GGATG GGATG(9/13)
540 GGATG
HaeII 263 AGCGCC [GA]GCGC^[CT]
842 GGCGCT
HapII 329 CCGG C^CGG
840 CCGG
HgaI 391 GACGC GACGC(5/10)
713 GCGTC
HinfI 177 GAATC G^A[ACGT]TC
337 GAGTC
HpaII 329 CCGG C^CGG
840 CCGG
MluNI 683 TGGCCA TGG^CCA
725 TGGCCA
MscI 683 TGGCCA TGG^CCA
725 TGGCCA
MspI 329 CCGG C^CGG
840 CCGG
MvnI 393 CGCG CG^CG
907 CGCG
PstI 146 CTGCAG CTGCA^G
802 CTGCAG
SfcI 146 CTGCAG C^T[GA][CT]AG
802 CTGCAG
SmlI 268 CTCGAG C^T[CT][GA]AG
344 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BsrI BssKI BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoRII EcoT14I Fnu4HI HaeIII
HhaI HinP1I HphI ItaI MaeIII
MboI MboII MnlI MspA1I MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI StyI TseI
Tsp45I TspRI XcmI