Restriction map for
pFN21AE4468
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I KasI KpnI KspI
MaeI MaeII MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Alw44I 1054 GTGCAC G^TGCAC
AocI 612 CCTCAGG CC^T[ACGT]AGG
ApaLI 1054 GTGCAC G^TGCAC
AvaI 779 CTCGGG C^[CT]CG[GA]G
AvaII 426 GGACC G^G[AT]CC
BalI 1227 TGGCCA TGG^CCA
BpmI 57 CTGGAG CTGGAG(16/14)
BsaWI 1001 ACCGGA [AT]^CCGG[AT]
BsgI 1183 CTGCAC GTGCAG(16/14)
BsmFI 782 GGGAC GGGAC(10/14)
BsoBI 779 CTCGGG C^[CT]CG[GA]G
BspHI 383 TCATGA T^CATGA
BspMI 1094 ACCTGC ACCTGC(4/8)
BsrI 301 CCAGT ACTGG(1/-1)
BssSI 1057 CACGAG CACGAG(-5/-1)
Bsu36I 612 CCTCAGG CC^T[ACGT]AGG
DraIII 485 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 631 CCATGG C^C[GA][CT]GG
EaeI 1227 TGGCCA [CT]^GGCC[GA]
Eco81I 612 CCTCAGG CC^T[ACGT]AGG
EcoNI 453 CCTTGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1198 GAATTC G^AATTC
EcoT22I 1421 ATGCAT ATGCA^T
HindIII 855 AAGCTT A^AGCTT
HpaI 316 GTTAAC GTT^AAC
MluNI 1227 TGGCCA TGG^CCA
MscI 1227 TGGCCA TGG^CCA
MwoI 1316 GCCTGGCCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 631 CCATGG C^CATGG
NsiI 1421 ATGCAT ATGCA^T
NspI 1031 ACATGC [GA]CATG^[CT]
PflMI 1230 CCACTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1021 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 127 CTGCAG CTGCA^G
PvuII 8 CAGCTG CAG^CTG
RcaI 383 TCATGA T^CATGA
SfcI 127 CTGCAG C^T[GA][CT]AG
Van91I 1230 CCACTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 497 CCACTTTCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AfaI 945 GTAC GT^AC
1446 GTAC
AlwNI 302 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1043 CAGATGCTG
BamHI 3 GGATCC G^GATCC
635 GGATCC
BbvI 449 GCTGC GCAGC(8/12)
1182 GCTGC
BclI 264 TGATCA T^GATCA
803 TGATCA
BsmAI 898 GAGAC GTCTC(1/5)
29 GTCTC
BspDI 554 ATCGAT AT^CGAT
667 ATCGAT
Cac8I 445 GCCCGC GC[ACGT]^[ACGT]GC
1321 GCCCGC
ClaI 554 ATCGAT AT^CGAT
667 ATCGAT
DraII 254 AGGCCCT [GA]G^G[ACGT]CC[CT]
1313 AGGGCCT
EcoO109I 254 AGGCCCT [GA]G^G[ACGT]CC[CT]
1313 AGGGCCT
EcoT14I 631 CCATGG C^C[AT][AT]GG
1145 CCAAGG
FauI 446 CCCGC CCCGC(4/6)
1322 CCCGC
FbaI 264 TGATCA T^GATCA
803 TGATCA
Fnu4HI 449 GCTGC GC^[ACGT]GC
1182 GCTGC
HapII 369 CCGG C^CGG
1002 CCGG
HincII 316 GTTAAC GT[CT]^[GA]AC
1384 GTTGAC
HindII 316 GTTAAC GT[CT]^[GA]AC
1384 GTTGAC
HpaII 369 CCGG C^CGG
1002 CCGG
HphI 484 TCACC GGTGA(8/7)
1406 TCACC
ItaI 449 GCTGC GC^[ACGT]GC
1182 GCTGC
MslI 219 CATTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
232 CATGGATATG
MspA1I 8 CAGCTG C[AC]G^C[GT]G
447 CCGCTG
MspI 369 CCGG C^CGG
1002 CCGG
RsaI 945 GTAC GT^AC
1446 GTAC
SacI 66 GAGCTC GAGCT^C
624 GAGCTC
SfaNI 1045 GATGC GCATC(5/9)
419 GCATC
StyI 631 CCATGG C^C[AT][AT]GG
1145 CCAAGG
TseI 449 GCTGC G^C[AT]GC
1182 GCTGC
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI BanII BmyI BsaJI BseRI
BsiHKAI BsiYI BslI Bsp1286I BsrDI
BssKI BstF5I BstNI BstYI Cfr13I
DdeI DpnII EarI EcoRII FokI
HaeIII HinfI Ksp632I MaeIII MboI
MboII MflI MnlI MseI MvaI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI TaqI TfiI Tru9I
Tsp45I Tsp509I TspRI TthHB8I XhoII