Restriction map for
pFN21AE4223
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII AccI AccIII AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaWI BseAI BsiHKAI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraIII DrdI
Eam1105I Eco47III EcoO65I EcoRV FseI
FspI HgaI HincII HindII HindIII
HpaI MluI MluNI MroI MscI
MslI NdeI NheI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PvuI RcaI
RsrII SacI SalI SapI SbfI
SexAI SfiI SfuI SmaI SpeI
Sse8387I SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 65 enzymes) #
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Acc65I 18 GGTACC G^GTACC
AclI 471 AACGTT AA^CGTT
AcyI 121 GGCGCC G[GA]^CG[CT]C
AocI 61 CCTGAGG CC^T[ACGT]AGG
AseI 553 ATTAAT AT^TAAT
AsnI 553 ATTAAT AT^TAAT
Asp718I 18 GGTACC G^GTACC
AvaII 664 GGTCC G^G[AT]CC
BanII 1596 GAGCCC G[GA]GC[CT]^C
BbeI 121 GGCGCC GGCGC^C
BmyI 1596 GAGCCC G[AGT]GC[ACT]^C
BsaAI 735 TACGTA [CT]AC^GT[GA]
BsaHI 121 GGCGCC G[GA]^CG[CT]C
BsaI 1224 GAGACC GGTCTC(1/5)
BsgI 236 CTGCAC GTGCAG(16/14)
BsmI 570 GAATGC GAATGC(1/-1)
Bsp1286I 1596 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 1848 TGTACA T^GTACA
BsrFI 124 GCCGGC [GA]^CCGG[CT]
BsrGI 1848 TGTACA T^GTACA
BstAPI 2007 GCATGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 32 CCACTCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2271 GGATCT [GA]^GATC[CT]
Bsu36I 61 CCTGAGG CC^T[ACGT]AGG
Cfr10I 124 GCCGGC [GA]^CCGG[CT]
DraII 347 AGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 53 CGGCCG [CT]^GGCC[GA]
EagI 53 CGGCCG C^GGCCG
EclXI 53 CGGCCG C^GGCCG
Eco52I 53 CGGCCG C^GGCCG
Eco81I 61 CCTGAGG CC^T[ACGT]AGG
EcoNI 2149 CCTGTAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 347 AGGCCCC [GA]G^G[ACGT]CC[CT]
HaeII 121 GGCGCC [GA]GCGC^[CT]
Hin1I 121 GGCGCC G[GA]^CG[CT]C
KasI 121 GGCGCC G^GCGCC
KpnI 18 GGTACC GGTAC^C
KspI 134 CCGCGG CCGC^GG
MfeI 844 CAATTG C^AATTG
MflI 2271 GGATCT [GA]^GATC[CT]
MunI 844 CAATTG C^AATTG
NaeI 124 GCCGGC GCC^GGC
NarI 121 GGCGCC GG^CGCC
NcoI 1689 CCATGG C^CATGG
NgoMIV 124 GCCGGC G^CCGGC
NotI 52 GCGGCCGC GC^GGCCGC
PflMI 1423 CCAGATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1660 GACTTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 553 ATTAAT AT^TAAT
Psp1406I 471 AACGTT AA^CGTT
PstI 2014 CTGCAG CTGCA^G
PvuII 191 CAGCTG CAG^CTG
SacII 134 CCGCGG CCGC^GG
ScaI 2023 AGTACT AGT^ACT
SfoI 121 GGCGCC GGC^GCC
SgrAI 123 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 735 TACGTA TAC^GTA
SphI 2007 GCATGC GCATG^C
SspBI 1848 TGTACA T^GTACA
TaqI 1215 TCGA T^CGA
Tsp45I 2138 GTGAC ^GT[GC]AC
TthHB8I 1215 TCGA T^CGA
Van91I 1423 CCAGATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 32 CCACTCCGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 2271 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 1035 GGATC GGATC(4/5)
2271 GGATC
BanI 18 GGTACC G^G[CT][GA]CC
121 GGCGCC
BclI 1617 TGATCA T^GATCA
2288 TGATCA
BcnI 1304 CCCGG CC^[GC]GG
1801 CCCGG
BsaBI 457 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1176 GATAAACATC
BsiEI 53 CGGCCG CG[GA][CT]^CG
131 CGACCG
BsmAI 1157 GAGAC GTCTC(1/5)
1224 GAGAC
BsmFI 1527 GGGAC GGGAC(10/14)
825 GTCCC
BspMI 234 ACCTGC ACCTGC(4/8)
2227 ACCTGC
BsrDI 1643 GCAATG GCAATG(2/0)
1721 GCAATG
BstNI 929 CCTGG CC^[AT]GG
1998 CCTGG
EarI 831 GAAGAG CTCTTC(1/4)
1963 GAAGAG
EcoRI 1501 GAATTC G^AATTC
1517 GAATTC
EcoRII 929 CCTGG ^CC[AT]GG
1998 CCTGG
EcoT14I 1553 CCTTGG C^C[AT][AT]GG
1689 CCATGG
EcoT22I 326 ATGCAT ATGCA^T
2005 ATGCAT
FbaI 1617 TGATCA T^GATCA
2288 TGATCA
Ksp632I 831 GAAGAG CTCTTC(1/4)
1963 GAAGAG
MamI 457 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1176 GATAAACATC
MvaI 929 CCTGG CC^[AT]GG
1998 CCTGG
NciI 1304 CCCGG CC^[GC]GG
1801 CCCGG
NsiI 326 ATGCAT ATGCA^T
2005 ATGCAT
NspI 1433 GCATGT [GA]CATG^[CT]
2007 GCATGC
SmlI 602 CTCAAG C^T[CT][GA]AG
2323 CTTGAG
StyI 1553 CCTTGG C^C[AT][AT]GG
1689 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI Asp700I BbsI BbvI BfaI
BpuAI BsaJI BseRI BsiYI BslI
BsrI BssKI BstF5I BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DsaI FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvnI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI Tru9I
TseI Tsp509I TspRI XmnI