##################################
# NO CUT ENZYMES (  159 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrGI       BssHII      BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       Bsu36I      
CelII       ClaI        CpoI        DraI        DraII       
DrdI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco52I      Eco81I      EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MspA1I      MunI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacII       SalI        SapI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        Tth111I     TthHB8I     XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AlwNI        1436  CAGTGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1137  AGCGCT               AGC^GCT
BamHI         176  GGATCC               G^GATCC
BbsI          649  GAAGAC               GAAGAC(2/6)
BfaI         1289  CTAG                 C^TAG
BpuAI         649  GAAGAC               GAAGAC(2/6)
BseRI        1240  CTCCTC               GAGGAG(10/8)
BsgI           90  CTGCAC               GTGCAG(16/14)
BsmBI         771  CGTCTC               CGTCTC(1/5)
BsmFI        1117  GGGAC                GGGAC(10/14)
BsmI         1234  GCATTC               GAATGC(1/-1)
BsrDI        1329  CATTGC               GCAATG(2/0)
BsrFI        1216  ACCGGC               [GA]^CCGG[CT]
Bst1107I     1150  GTATAC               GTA^TAC
BstYI         176  GGATCC               [GA]^GATC[CT]
BstZ17I      1150  GTATAC               GTA^TAC
CfoI         1138  GCGC                 GCG^C
Cfr10I       1216  ACCGGC               [GA]^CCGG[CT]
DraIII       1162  CACGCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     1137  AGCGCT               AGC^GCT
EcoNI         575  CCTTGATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       395  CCATGG               C^C[AT][AT]GG
HaeII        1137  AGCGCT               [GA]GCGC^[CT]
HaeIII        584  GGCC                 GG^CC
HapII        1217  CCGG                 C^CGG
HhaI         1138  GCGC                 GCG^C
HinP1I       1138  GCGC                 G^CGC
HpaII        1217  CCGG                 C^CGG
HphI         1179  GGTGA                GGTGA(8/7)
MaeI         1289  CTAG                 C^TAG
MflI          176  GGATCC               [GA]^GATC[CT]
MslI          914  CATTTGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI         1217  CCGG                 C^CGG
NcoI          395  CCATGG               C^CATGG
PflMI         686  CCAAAGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI          407  AGTACT               AGT^ACT
StyI          395  CCATGG               C^C[AT][AT]GG
Van91I        686  CCAAAGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         176  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AccI           24  GTCTAC               GT^[AC][GT]AC
             1150  GTATAC              
AciI          519  GCGG                 CCGC(-3/-1)
              660  GCGG                
BsaJI         395  CCATGG               C^C[ACGT][ACGT]GG
              977  CCGTGG              
BssSI         315  CACGAG               CACGAG(-5/-1)
             1036  CTCGTG              
DsaI          395  CCATGG               C^C[GA][CT]GG
              977  CCGTGG              
FauI          519  GCGGG                CCCGC(4/6)
              660  GCGGG               
MboII         649  GAAGA                GAAGA(8/7)
              945  GAAGA               
MseI          596  TTAA                 T^TAA
              656  TTAA                
SacI           17  GAGCTC               GAGCT^C
              303  GAGCTC              
SfaNI         283  GCATC                GCATC(5/9)
              327  GCATC               
Tru9I         596  TTAA                 T^TAA
              656  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AfaI        AluI        AlwI        AspHI       AvaII       
BanI        BanII       BbvI        BmyI        BpmI        
BsiHKAI     BsiYI       BslI        BsmAI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       EcoRII      Fnu4HI      
FokI        HinfI       ItaI        MaeIII      MboI        
MnlI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfcI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       XcmI