##################################
# NO CUT ENZYMES (  206 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BciVI       BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstNI       BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT14I     EcoT22I     FauI        FseI        FspI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MslI        MspI        MunI        
MvaI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsaI        RsrII       SacI        SacII       
SalI        Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        Tsp509I     Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   24 enzymes) #
###################################

AciI          282  GCGG                 CCGC(-3/-1)
AlwI           58  GGATC                GGATC(4/5)
AlwNI         301  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
BbvCI          23  CCTCAGC              CCTCAGC(-5/-2)
BcgI          222  GCAAGTTGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          216  TGATCA               T^GATCA
BfaI          244  CTAG                 C^TAG
BsaJI          91  CCACGG               C^C[ACGT][ACGT]GG
BseRI          21  CTCCTC               GAGGAG(10/8)
BsmAI          73  GTCTC                GTCTC(1/5)
BsrI           55  ACTGG                ACTGG(1/-1)
BstYI          58  GGATCT               [GA]^GATC[CT]
DsaI           91  CCACGG               C^C[GA][CT]GG
FbaI          216  TGATCA               T^GATCA
MaeI          244  CTAG                 C^TAG
MaeIII         52  GTCAC                ^GT[ACGT]AC
MflI           58  GGATCT               [GA]^GATC[CT]
NlaIII        214  CATG                 CATG^
SapI          259  GAAGAGC              GCTCTTC(1/4)
SfaNI         190  GCATC                GCATC(5/9)
TaqI          296  TCGA                 T^CGA
Tsp45I         52  GTCAC                ^GT[GC]AC
TthHB8I       296  TCGA                 T^CGA
XhoII          58  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

BpmI          125  CTGGAG               CTGGAG(16/14)
              265  CTGGAG              
BsiYI          86  CCCCACCACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              319  CCCTCTTCAGG         
BslI           86  CCCCACCACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              319  CCCTCTTCAGG         
DpnII          59  GATC                 ^GATC
              217  GATC                
EarI          321  CTCTTC               CTCTTC(1/4)
              259  GAAGAG              
Ksp632I       321  CTCTTC               CTCTTC(1/4)
              259  GAAGAG              
MboI           59  GATC                 ^GATC
              217  GATC                
MseI          200  TTAA                 T^TAA
              287  TTAA                
MspA1I         26  CAGCTG               C[AC]G^C[GT]G
              301  CAGCTG              
MwoI          282  GCGGTTTAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              291  GCCCATCGAGC         
NdeII          59  GATC                 ^GATC
              217  GATC                
PvuII          26  CAGCTG               CAG^CTG
              301  CAGCTG              
Sau3AI         59  GATC                 ^GATC
              217  GATC                
Tru9I         200  TTAA                 T^TAA
              287  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AluI        BbvI        BstF5I      DdeI        Fnu4HI      
FokI        HinfI       HphI        ItaI        MboII       
MnlI        TfiI        TseI        TspRI