Restriction map for
pFN21AE2295
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# NO CUT ENZYMES ( 71 enzymes) #
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AatII Acc65I AccII AccIII AcyI
Aor51HI AscI Asp718I AspI AviII
BbeI BciVI BlpI Bpu1102I BsaHI
BseAI BsiWI BspDI BspEI BssHII
BstEII BstPI BstUI CelII ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoO65I FseI FspI Hin1I KasI
KpnI KspI MluI MroI MvnI
NaeI NarI NgoMIV NheI NotI
NruI PacI PflFI PmeI PshAI
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AclI 2461 AACGTT AA^CGTT
AflII 838 CTTAAG C^TTAAG
AgeI 4414 ACCGGT A^CCGGT
Alw44I 4169 GTGCAC G^TGCAC
AocI 5399 CCTTAGG CC^T[ACGT]AGG
ApaI 6388 GGGCCC GGGCC^C
ApaLI 4169 GTGCAC G^TGCAC
AvrII 1092 CCTAGG C^CTAGG
BalI 6026 TGGCCA TGG^CCA
BamHI 861 GGATCC G^GATCC
BbrPI 2161 CACGTG CAC^GTG
BbvCI 5947 GCTGAGG CCTCAGC(-5/-2)
BcgI 1047 CGAAGCTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 6068 CCCGG CC^[GC]GG
BfrI 838 CTTAAG C^TTAAG
BglI 2677 GCCATAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1092 CCTAGG C^CTAGG
BsaAI 2161 CACGTG [CT]AC^GT[GA]
BsgI 6079 CTGCAC GTGCAG(16/14)
BsiEI 4590 CGACCG CG[GA][CT]^CG
BsmBI 962 CGTCTC CGTCTC(1/5)
BspHI 4023 TCATGA T^CATGA
BssSI 824 CACGAG CACGAG(-5/-1)
Bst1107I 2543 GTATAC GTA^TAC
BstBI 1045 TTCGAA TT^CGAA
BstZ17I 2543 GTATAC GTA^TAC
Bsu36I 5399 CCTTAGG CC^T[ACGT]AGG
CfoI 313 GCGC GCG^C
DsaI 2012 CCATGG C^C[GA][CT]GG
Eco81I 5399 CCTTAGG CC^T[ACGT]AGG
EcoRV 3737 GATATC GAT^ATC
EcoT22I 3988 ATGCAT ATGCA^T
HaeII 312 GGCGCT [GA]GCGC^[CT]
HgaI 5593 GACGC GACGC(5/10)
HhaI 313 GCGC GCG^C
HinP1I 313 GCGC G^CGC
MluNI 6026 TGGCCA TGG^CCA
MscI 6026 TGGCCA TGG^CCA
NciI 6068 CCCGG CC^[GC]GG
NcoI 2012 CCATGG C^CATGG
NdeI 1370 CATATG CA^TATG
NsiI 3988 ATGCAT ATGCA^T
NspV 1045 TTCGAA TT^CGAA
PaeR7I 5066 CTCGAG C^TCGAG
PinAI 4414 ACCGGT A^CCGGT
PmaCI 2161 CACGTG CAC^GTG
PmlI 2161 CACGTG CAC^GTG
PpuMI 5204 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 2461 AACGTT AA^CGTT
RcaI 4023 TCATGA T^CATGA
SfuI 1045 TTCGAA TT^CGAA
XhoI 5066 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccI 957 GTCTAC GT^[AC][GT]AC
2543 GTATAC
AhdI 1376 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5932 GACCCCCTGTC
AseI 153 ATTAAT AT^TAAT
5764 ATTAAT
AsnI 153 ATTAAT AT^TAAT
5764 ATTAAT
Asp700I 3822 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4486 GAAAAAGTTC
AspEI 1376 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5932 GACCCCCTGTC
AvaI 4934 CCCGAG C^[CT]CG[GA]G
5066 CTCGAG
BglII 1179 AGATCT A^GATCT
1850 AGATCT
BsaBI 600 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3100 GATTTGCATC
BsaI 1171 GAGACC GGTCTC(1/5)
5416 GAGACC
BsaWI 4038 ACCGGA [AT]^CCGG[AT]
4414 ACCGGT
BsoBI 4934 CCCGAG C^[CT]CG[GA]G
5066 CTCGAG
BsrBI 5079 CCGCTC CCGCTC(-3/-3)
5507 CCGCTC
BsrFI 4414 ACCGGT [GA]^CCGG[CT]
4592 ACCGGC
BstXI 1117 CCACATCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2475 CCAGCAGCCTGG
Cfr10I 4414 ACCGGT [GA]^CCGG[CT]
4592 ACCGGC
DraI 1962 TTTAAA TTT^AAA
2320 TTTAAA
EaeI 4586 TGGCCG [CT]^GGCC[GA]
6026 TGGCCA
Eam1105I 1376 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5932 GACCCCCTGTC
EcoNI 1092 CCTAGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1555 CCTGAGAGAGG
EcoRI 2341 GAATTC G^AATTC
2718 GAATTC
HpaI 2151 GTTAAC GTT^AAC
3861 GTTAAC
MamI 600 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3100 GATTTGCATC
PshBI 153 ATTAAT AT^TAAT
5764 ATTAAT
XmnI 3822 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4486 GAAAAAGTTC
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# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BanII BbsI BbvI BclI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspLU11I
BspMI BsrDI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII DraIII
DrdI EarI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MnlI MseI MslI MspA1I
MspI MunI MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII