Restriction map for
pFN21AE5730
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# NO CUT ENZYMES ( 102 enzymes) #
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Acc65I AccI AccIII AclI AflII
AhdI AocI AscI Asp718I AspEI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BfrI BglII
BlnI BsaBI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HpaI HphI KasI KpnI
KspI MamI MroI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI StyI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 705 GACGTC GACGT^C
AcsI 1115 AAATTC [GA]^AATT[CT]
AcyI 705 GACGTC G[GA]^CG[CT]C
AgeI 1598 ACCGGT A^CCGGT
Alw44I 1002 GTGCAC G^TGCAC
Aor51HI 791 AGCGCT AGC^GCT
ApaI 155 GGGCCC GGGCC^C
ApaLI 1002 GTGCAC G^TGCAC
ApoI 1115 AAATTC [GA]^AATT[CT]
AseI 601 ATTAAT AT^TAAT
AsnI 601 ATTAAT AT^TAAT
AspI 1060 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 339 GCTGAGG CCTCAGC(-5/-2)
BlpI 837 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 837 GCTCAGC GC^T[ACGT]AGC
BsaAI 78 CACGTA [CT]AC^GT[GA]
BsaHI 705 GACGTC G[GA]^CG[CT]C
BsaWI 1598 ACCGGT [AT]^CCGG[AT]
BspLU11I 248 ACATGT A^CATGT
BsrBI 199 GAGCGG CCGCTC(-3/-3)
BstAPI 87 GCAGTGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 627 GGATCT [GA]^GATC[CT]
CelII 837 GCTCAGC GC^T[ACGT]AGC
DraIII 100 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1174 GACTTCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1634 CCACGG C^C[GA][CT]GG
Eco47III 791 AGCGCT AGC^GCT
EcoNI 1271 CCTTCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 791 AGCGCT [GA]GCGC^[CT]
Hin1I 705 GACGTC G[GA]^CG[CT]C
HincII 280 GTCAAC GT[CT]^[GA]AC
HindII 280 GTCAAC GT[CT]^[GA]AC
HindIII 1432 AAGCTT A^AGCTT
MaeIII 1396 GTGAC ^GT[ACGT]AC
MfeI 692 CAATTG C^AATTG
MflI 627 GGATCT [GA]^GATC[CT]
MluI 1341 ACGCGT A^CGCGT
MunI 692 CAATTG C^AATTG
PflFI 1060 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1598 ACCGGT A^CCGGT
PshBI 601 ATTAAT AT^TAAT
ScaI 233 AGTACT AGT^ACT
SmaI 1538 CCCGGG CCC^GGG
SmlI 1018 CTCAAG C^T[CT][GA]AG
SphI 92 GCATGC GCATG^C
TaqI 980 TCGA T^CGA
TfiI 1328 GATTC G^A[AT]TC
Tsp45I 1396 GTGAC ^GT[GC]AC
Tth111I 1060 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 980 TCGA T^CGA
XcmI 1487 CCATAACAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 627 GGATCT [GA]^GATC[CT]
XmaCI 1538 CCCGGG C^CCGGG
XmaI 1538 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AflIII 248 ACATGT A^C[GA][CT]GT
1341 ACGCGT
AlwI 1050 GATCC GGATC(4/5)
627 GGATC
AlwNI 67 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
1421 CAGCTTCTG
Asp700I 1144 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1206 GAAAACATTC
AvaI 1538 CCCGGG C^[CT]CG[GA]G
1561 CCCGAG
BbsI 210 GAAGAC GAAGAC(2/6)
1289 GAAGAC
BglI 938 GCCGAGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1132 GCCTCCTCGGC
BpuAI 210 GAAGAC GAAGAC(2/6)
1289 GAAGAC
BsgI 142 GTGCAG GTGCAG(16/14)
997 GTGCAG
BsmBI 613 CGTCTC CGTCTC(1/5)
941 GAGACG
BsoBI 1538 CCCGGG C^[CT]CG[GA]G
1561 CCCGAG
BsrFI 485 ACCGGC [GA]^CCGG[CT]
1598 ACCGGT
BssSI 1863 CACGAG CACGAG(-5/-1)
748 CTCGTG
Cfr10I 485 ACCGGC [GA]^CCGG[CT]
1598 ACCGGT
EarI 472 CTCTTC CTCTTC(1/4)
126 GAAGAG
Ksp632I 472 CTCTTC CTCTTC(1/4)
126 GAAGAG
MseI 602 TTAA T^TAA
1217 TTAA
NspI 92 GCATGC [GA]CATG^[CT]
248 ACATGT
SacI 835 GAGCTC GAGCT^C
1072 GAGCTC
SapI 126 GAAGAGC GCTCTTC(1/4)
471 GCTCTTC
Tru9I 602 TTAA T^TAA
1217 TTAA
XmnI 1144 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1206 GAAAACATTC
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaII BalI BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII ItaI MaeI
MaeII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TseI Tsp509I TspRI Van91I